Overview
| Uniprot ID | Q863I2 |
|---|
| Protein Name | Serine/threonine-protein kinase OSR1 |
|---|
| Gene Name | OXSR1 |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 297 |
FFQKAKNKEYLQEKI |
Function
Effector serine/threonine-protein kinase component of the WNK-SPAK/OSR1 kinase cascade, which is involved in various processes, such as ion transport, response to hypertonic stress and blood pressure (By similarity). Specifically recognizes and binds proteins with a RFXV motif (By similarity). Acts downstream of WNK kinases (WNK1, WNK2, WNK3 or WNK4): following activation by WNK kinases, catalyzes phosphorylation of ion cotransporters, such as SLC12A1/NKCC2, SLC12A2/NKCC1, SLC12A3/NCC, SLC12A5/KCC2 or SLC12A6/KCC3, regulating their activity (By similarity). Mediates regulatory volume increase in response to hyperosmotic stress by catalyzing phosphorylation of ion cotransporters SLC12A1/NKCC2, SLC12A2/NKCC1 and SLC12A6/KCC3 downstream of WNK1 and WNK3 kinases (By similarity). Phosphorylation of Na-K-Cl cotransporters SLC12A2/NKCC1 and SLC12A2/NKCC1 promote their activation and ion influx; simultaneously, phosphorylation of K-Cl cotransporters SLC12A5/KCC2 and SLC12A6/KCC3 inhibit their activity, blocking ion efflux (By similarity). Acts as a regulator of NaCl reabsorption in the distal nephron by mediating phosphorylation and activation of the thiazide-sensitive Na-Cl cotransporter SLC12A3/NCC in distal convoluted tubule cells of kidney downstream of WNK4 (By similarity). Also acts as a regulator of angiogenesis in endothelial cells downstream of WNK1 (By similarity). Acts as an activator of inward rectifier potassium channels KCNJ2/Kir2.1 and KCNJ4/Kir2.3 downstream of WNK1: recognizes and binds the RXFXV/I variant motif on KCNJ2/Kir2.1 and KCNJ4/Kir2.3 and regulates their localization to the cell membrane without mediating their phosphorylation (By similarity). Phosphorylates RELL1, RELL2, RELT and PAK1. Phosphorylates PLSCR1 in the presence of RELT (By similarity)
Protein Sequence
10
MSEDSSALPW
20
SINKDDYELQ
30
EVIGSGATAV
40
VQAAYCTPKK
50
EKVAIKRINL
60
EKCQTSMDEL
70
LKEIQAMSQC
80
HHPNIVSYYT
90
SFVVKDELWL
100
VMKLLSGGSV
110
LDIIKHIVAK
120
GEHKSGVLDE
130
ATIATILREV
140
LEGLEYLHKN
150
GQIHRDVKAG
160
NILLGEDGSV
170
QIADFGVSAF
180
LATGGDITRN
190
KVRKTFVGTP
200
CWMAPEVMEQ
210
VRGYDFKADI
220
WSFGITAIEL
230
ATGAAPYHKY
240
PPMKVLMLTL
250
QNDPPSLETG
260
VQDKEMLKKY
270
GKSFRKMISL
280
CLQKDPEKRP
290
TAAELLRHKF
300
FQKAKNKEYL
310
QEKILQRAPT
320
ISERAKKVRR
330
VPGSSGRLHK
340
TEDGGWEWSD
350
DEFDEESEEG
360
KAAISQLRSP
370
RVKESLTNSE
380
LFSTTHPVGT
390
LLQVPEQISA
400
HLPQSAGQMP
410
AQLTPVSLPP
420
AAELAPVQAA
430
QAQSSGAGSQ
440
ETKIPISLVL
450
RLRNSKKELN
460
DIRFEFTPGR
470
DTAEGVSQEL
480
ISAGLVDGRD
490
LVIVAANLQK
500
IVEEPQSNRS
510
VTFKLASGVE
520
GSDIPDDSKL
IGFAQLSIS
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0005829 |
cytosol |
| Molecular Function |
GO:0005524 |
ATP binding |
| Molecular Function |
GO:0000287 |
magnesium ion binding |
| Molecular Function |
GO:0106310 |
protein serine kinase activity |
| Molecular Function |
GO:0004674 |
protein serine/threonine kinase activity |
| Biological Process |
GO:0071474 |
cellular hyperosmotic response |
| Biological Process |
GO:0035556 |
intracellular signal transduction |
| Biological Process |
GO:0010820 |
positive regulation of T cell chemotaxis |
| Biological Process |
GO:0046777 |
protein autophosphorylation |
| Biological Process |
GO:0006468 |
protein phosphorylation |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.