Search Results

Overview

Uniprot IDQ86UE8
Protein NameSerine/threonine-protein kinase tousled-like 2
Gene NameTLK2
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
66 DQRNRKRKAEPYETS
76 PYETSQGKGTPRGHK

Function

Serine/threonine-protein kinase involved in the process of chromatin assembly and probably also DNA replication, transcription, repair, and chromosome segregation (PubMed:10523312, PubMed:11470414, PubMed:12660173, PubMed:12955071, PubMed:29955062, PubMed:33323470, PubMed:9427565). Phosphorylates the chromatin assembly factors ASF1A and ASF1B (PubMed:11470414, PubMed:20016786, PubMed:29955062, PubMed:35136069). Phosphorylation of ASF1A prevents its proteasome-mediated degradation, thereby enhancing chromatin assembly (PubMed:20016786). Negative regulator of amino acid starvation-induced autophagy (PubMed:22354037)

Protein Sequence

10 MMEELHSLDP 20 RRQELLEARF 30 TGVGVSKGPL 40 NSESSNQSLC 50 SVGSLSDKEV 60 ETPEKKQNDQ 70 RNRKRKAEPY 80 ETSQGKGTPR 90 GHKISDYFEF 100 AGGSAPGTSP 110 GRSVPPVARS 120 SPQHSLSNPL 130 PRRVEQPLYG 140 LDGSAAKEAT 150 EEQSALPTLM 160 SVMLAKPRLD 170 TEQLAQRGAG 180 LCFTFVSAQQ 190 NSPSSTGSGN 200 TEHSCSSQKQ 210 ISIQHRQTQS 220 DLTIEKISAL 230 ENSKNSDLEK 240 KEGRIDDLLR 250 ANCDLRRQID 260 EQQKMLEKYK 270 ERLNRCVTMS 280 KKLLIEKSKQ 290 EKMACRDKSM 300 QDRLRLGHFT 310 TVRHGASFTE 320 QWTDGYAFQN 330 LIKQQERINS 340 QREEIERQRK 350 MLAKRKPPAM 360 GQAPPATNEQ 370 KQRKSKTNGA 380 ENETPSSGNT 390 ELKDTAPALG 400 AHSLLRLTLA 410 EYHEQEEIFK 420 LRLGHLKKEE 430 AEIQAELERL 440 ERVRNLHIRE 450 LKRIHNEDNS 460 QFKDHPTLND 470 RYLLLHLLGR 480 GGFSEVYKAF 490 DLTEQRYVAV 500 KIHQLNKNWR 510 DEKKENYHKH 520 ACREYRIHKE 530 LDHPRIVKLY 540 DYFSLDTDSF 550 CTVLEYCEGN 560 DLDFYLKQHK 570 LMSEKEARSI 580 IMQIVNALKY 590 LNEIKPPIIH 600 YDLKPGNILL 610 VNGTACGEIK 620 ITDFGLSKIM 630 DDDSYNSVDG 640 MELTSQGAGT 650 YWYLPPECFV 660 VGKEPPKISN 670 KVDVWSVGVI 680 FYQCLYGRKP 690 FGHNQSQQDI 700 LQENTILKAT 710 EVQFPPKPVV 720 TPEAKAFIRR 730 CLAYRKEDRI 740 DVQQLACDPY 750 LLPHIRKSVS 760 TSSPAGAAIA 770 STSGASNNSS SN

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005882 intermediate filament
Cellular Component GO:0005730 nucleolus
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0048471 perinuclear region of cytoplasm
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0106310 protein serine kinase activity
Molecular Function GO:0004674 protein serine/threonine kinase activity
Biological Process GO:0071480 cellular response to gamma radiation
Biological Process GO:0006325 chromatin organization
Biological Process GO:0007059 chromosome segregation
Biological Process GO:0006974 DNA damage response
Biological Process GO:0035556 intracellular signal transduction
Biological Process GO:0010507 negative regulation of autophagy
Biological Process GO:0032435 negative regulation of proteasomal ubiquitin-dependent protein catabolic process
Biological Process GO:0051647 nucleus localization
Biological Process GO:0018105 peptidyl-serine phosphorylation
Biological Process GO:0006468 protein phosphorylation
Biological Process GO:1902275 regulation of chromatin organization

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.