Overview
| Uniprot ID | Q86W56 |
|---|
| Protein Name | Poly(ADP-ribose) glycohydrolase |
|---|
| Gene Name | PARG |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 156 |
HQTAAMCKWQNEGKH |
| 409 |
QHGKKDSKITDHFMR |
| 650 |
PRRNAKMKSEYSSYP |
Function
Poly(ADP-ribose) glycohydrolase that degrades poly(ADP-ribose) by hydrolyzing the ribose-ribose bonds present in poly(ADP-ribose) (PubMed:15450800, PubMed:21892188, PubMed:23102699, PubMed:23474714, PubMed:33186521, PubMed:34019811, PubMed:34321462). Mainly acts as an exo-glycohydrolase but can act as an endo-glycohydrolase at low efficiency, releasing poly(ADP-ribose) of different length as well as ADP-ribose monomers (PubMed:23102699, PubMed:23481255, PubMed:37268618). It is however unable to cleave the ester bond between the terminal ADP-ribose and ADP-ribosylated residues, leaving proteins that are mono-ADP-ribosylated (PubMed:21892188, PubMed:23474714, PubMed:33186521, PubMed:37268618). Poly(ADP-ribose) synthesized after DNA damage is only present transiently and is rapidly degraded by PARG (PubMed:23102699, PubMed:34019811). Required to prevent detrimental accumulation of poly(ADP-ribose) upon prolonged replicative stress, while it is not required for recovery from transient replicative stress (PubMed:24906880). Responsible for the prevalence of mono-ADP-ribosylated proteins in cells, thanks to its ability to degrade poly(ADP-ribose) without cleaving the terminal protein-ribose bond (PubMed:33186521). Required for retinoid acid-dependent gene transactivation, probably by removing poly(ADP-ribose) from histone demethylase KDM4D, allowing chromatin derepression at RAR-dependent gene promoters (PubMed:23102699). Involved in the synthesis of ATP in the nucleus, together with PARP1, NMNAT1 and NUDT5 (PubMed:27257257). Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming (PubMed:27257257)
Protein Sequence
10
MNAGPGCEPC
20
TKRPRWGAAT
30
TSPAASDARS
40
FPSRQRRVLD
50
PKDAHVQFRV
60
PPSSPACVPG
70
RAGQHRGSAT
80
SLVFKQKTIT
90
SWMDTKGIKT
100
AESESLDSKE
110
NNNTRIESMM
120
SSVQKDNFYQ
130
HNVEKLENVS
140
QLSLDKSPTE
150
KSTQYLNQHQ
160
TAAMCKWQNE
170
GKHTEQLLES
180
EPQTVTLVPE
190
QFSNANIDRS
200
PQNDDHSDTD
210
SEENRDNQQF
220
LTTVKLANAK
230
QTTEDEQARE
240
AKSHQKCSKS
250
CDPGEDCASC
260
QQDEIDVVPE
270
SPLSDVGSED
280
VGTGPKNDNK
290
LTRQESCLGN
300
SPPFEKESEP
310
ESPMDVDNSK
320
NSCQDSEADE
330
ETSPGFDEQE
340
DGSSSQTANK
350
PSRFQARDAD
360
IEFRKRYSTK
370
GGEVRLHFQF
380
EGGESRTGMN
390
DLNAKLPGNI
400
SSLNVECRNS
410
KQHGKKDSKI
420
TDHFMRLPKA
430
EDRRKEQWET
440
KHQRTERKIP
450
KYVPPHLSPD
460
KKWLGTPIEE
470
MRRMPRCGIR
480
LPLLRPSANH
490
TVTIRVDLLR
500
AGEVPKPFPT
510
HYKDLWDNKH
520
VKMPCSEQNL
530
YPVEDENGER
540
TAGSRWELIQ
550
TALLNKFTRP
560
QNLKDAILKY
570
NVAYSKKWDF
580
TALIDFWDKV
590
LEEAEAQHLY
600
QSILPDMVKI
610
ALCLPNICTQ
620
PIPLLKQKMN
630
HSITMSQEQI
640
ASLLANAFFC
650
TFPRRNAKMK
660
SEYSSYPDIN
670
FNRLFEGRSS
680
RKPEKLKTLF
690
CYFRRVTEKK
700
PTGLVTFTRQ
710
SLEDFPEWER
720
CEKPLTRLHV
730
TYEGTIEENG
740
QGMLQVDFAN
750
RFVGGGVTSA
760
GLVQEEIRFL
770
INPELIISRL
780
FTEVLDHNEC
790
LIITGTEQYS
800
EYTGYAETYR
810
WSRSHEDGSE
820
RDDWQRRCTE
830
IVAIDALHFR
840
RYLDQFVPEK
850
MRRELNKAYC
860
GFLRPGVSSE
870
NLSAVATGNW
880
GCGAFGGDAR
890
LKALIQILAA
900
AAAERDVVYF
910
TFGDSELMRD
920
IYSMHIFLTE
930
RKLTVGDVYK
940
LLLRYYNEEC
950
RNCSTPGPDI
960
KLYPFIYHAV
970
ESCAETADHS
GQRTGT
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0005759 |
mitochondrial matrix |
| Cellular Component |
GO:0016604 |
nuclear body |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Molecular Function |
GO:0004649 |
poly(ADP-ribose) glycohydrolase activity |
| Biological Process |
GO:1990966 |
ATP generation from poly-ADP-D-ribose |
| Biological Process |
GO:0006287 |
base-excision repair, gap-filling |
| Biological Process |
GO:0005975 |
carbohydrate metabolic process |
| Biological Process |
GO:0009225 |
nucleotide-sugar metabolic process |
| Biological Process |
GO:0006282 |
regulation of DNA repair |
Reference
PMID: N/A