Search Results

Overview

Uniprot ID	Q86WU2
Protein Name	D-lactate dehydrogenase, mitochondrial
Gene Name	LDHD
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
141	VEPGVTRKALNAHLR
24	GYCSQKAKGELCRDF
472	EHGIGMGKRQLLQEE

Function

The mitochondrial D-lactate dehydrogenase is a stereoselective dehydrogenase that targets a wide variety of D-2-hydroxyacids, particularly those with small to moderately sized hydrophobic groups attached to the C2 atom. It includes D-lactate which is generated in small amounts either endogenously through the methylglyoxal metabolism pathway or exogenously via intestinal bacterial activity and dietary intake. The dehydrogenase acts specifically on D-lactate, not on its stereoisomer L-lactate, and prevents the toxic accumulation of D-lactate in the organism (PubMed:30931947, PubMed:38373542). By converting branched-chain D-2-hydroxyacids into branched-chain ketoacids, it may indirectly regulate branched-chain amino acid metabolism (By similarity)

Protein Sequence

10 MARLLRSATW 20 ELFPWRGYCS 30 QKAKGELCRD 40 FVEALKAVVG 50 GSHVSTAAVV 60 REQHGRDESV 70 HRCEPPDAVV 80 WPQNVEQVSR 90 LAALCYRQGV 100 PIIPFGTGTG 110 LEGGVCAVQG 120 GVCVNLTHMD 130 RILELNQEDF 140 SVVVEPGVTR 150 KALNAHLRDS 160 GLWFPVDPGA 170 DASLCGMAAT 180 GASGTNAVRY 190 GTMRDNVLNL 200 EVVLPDGRLL 210 HTAGRGRHFR 220 FGFWPEIPHH 230 TAWYSPCVSL 240 GRRKSAAGYN 250 LTGLFVGSEG 260 TLGLITATTL 270 RLHPAPEATV 280 AATCAFPSVQ 290 AAVDSTVHIL 300 QAAVPVARIE 310 FLDEVMMDAC 320 NRYSKLNCLV 330 APTLFLEFHG 340 SQQALEEQLQ 350 RTEEIVQQNG 360 ASDFSWAKEA 370 EERSRLWTAR 380 HNAWYAALAT 390 RPGCKGYSTD 400 VCVPISRLPE 410 IVVQTKEDLN 420 ASGLTGSIVG 430 HVGDGNFHCI 440 LLVNPDDAEE 450 LGRVKAFAEQ 460 LGRRALALHG 470 TCTGEHGIGM 480 GKRQLLQEEV 490 GAVGVETMRQ 500 LKAVLDPQGL MNPGKVL

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005743	mitochondrial inner membrane
Cellular Component	GO:0005739	mitochondrion
Molecular Function	GO:0140174	(2R)-2-hydroxycarboxylate dehydrogenase activity
Molecular Function	GO:0004458	D-lactate dehydrogenase (cytochrome) activity
Molecular Function	GO:0140170	D-lactate dehydrogenase (FAD) activity
Molecular Function	GO:0008720	D-lactate dehydrogenase (NAD+) activity
Molecular Function	GO:0071949	FAD binding
Molecular Function	GO:0050660	flavin adenine dinucleotide binding
Molecular Function	GO:0004457	lactate dehydrogenase activity
Molecular Function	GO:0046872	metal ion binding
Biological Process	GO:1903457	lactate catabolic process

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.