Search Results

Overview

Uniprot IDQ86YP4
Protein NameTranscriptional repressor p66-alpha
Gene NameGATAD2A
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
204 TQNIPAGKPSLQTSS
233 GGQQASSKLGPQASS
28 EDDVESKKIKMERGL
349 ASRQAAAKLALRKQL
451 TNQKKALKVEHTSRL
459 VEHTSRLKAAFVKAL
464 RLKAAFVKALQQEQE
487 GTAPAQAKAEPTAAP
499 AAPHPVLKQVIKPRR
503 PVLKQVIKPRRKLAF
550 HTFSPSPKLQNSASA
576 ERTVSAGKGSATSNW
584 GSATSNWKKTPLSTG
585 SATSNWKKTPLSTGG
605 SPSLAVHKSSSAVDR
93 RTSHSDMKSERRPPS

Function

Transcriptional repressor (PubMed:12183469, PubMed:16415179). Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed:16428440, PubMed:28977666). Enhances MBD2-mediated repression (PubMed:12183469, PubMed:16415179). Efficient repression requires the presence of GATAD2B (PubMed:16415179)

Protein Sequence

10 MTEEACRTRS 20 QKRALERDPT 30 EDDVESKKIK 40 MERGLLASDL 50 NTDGDMRVTP 60 EPGAGPTQGL 70 LRATEATAMA 80 MGRGEGLVGD 90 GPVDMRTSHS 100 DMKSERRPPS 110 PDVIVLSDNE 120 QPSSPRVNGL 130 TTVALKETST 140 EALMKSSPEE 150 RERMIKQLKE 160 ELRLEEAKLV 170 LLKKLRQSQI 180 QKEATAQKPT 190 GSVGSTVTTP 200 PPLVRGTQNI 210 PAGKPSLQTS 220 SARMPGSVIP 230 PPLVRGGQQA 240 SSKLGPQASS 250 QVVMPPLVRG 260 AQQIHSIRQH 270 SSTGPPPLLL 280 APRASVPSVQ 290 IQGQRIIQQG 300 LIRVANVPNT 310 SLLVNIPQPT 320 PASLKGTTAT 330 SAQANSTPTS 340 VASVVTSAES 350 PASRQAAAKL 360 ALRKQLEKTL 370 LEIPPPKPPA 380 PEMNFLPSAA 390 NNEFIYLVGL 400 EEVVQNLLET 410 QGRMSAATVL 420 SREPYMCAQC 430 KTDFTCRWRE 440 EKSGAIMCEN 450 CMTTNQKKAL 460 KVEHTSRLKA 470 AFVKALQQEQ 480 EIEQRLLQQG 490 TAPAQAKAEP 500 TAAPHPVLKQ 510 VIKPRRKLAF 520 RSGEARDWSN 530 GAVLQASSQL 540 SRGSATTPRG 550 VLHTFSPSPK 560 LQNSASATAL 570 VSRTGRHSER 580 TVSAGKGSAT 590 SNWKKTPLST 600 GGTLAFVSPS 610 LAVHKSSSAV 620 DRQREYLLDM 630 IPPRSIPQSA TWK

Gene Ontology

Classification GO ID Description
Cellular Component GO:0016607 nuclear speck
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0016581 NuRD complex
Molecular Function GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific
Molecular Function GO:0030674 protein-macromolecule adaptor activity
Molecular Function GO:0043565 sequence-specific DNA binding
Molecular Function GO:0008270 zinc ion binding
Biological Process GO:0006338 chromatin remodeling
Biological Process GO:0006351 DNA-templated transcription
Biological Process GO:0045892 negative regulation of DNA-templated transcription
Biological Process GO:0000122 negative regulation of transcription by RNA polymerase II
Biological Process GO:0045893 positive regulation of DNA-templated transcription
Biological Process GO:0042659 regulation of cell fate specification
Biological Process GO:2000736 regulation of stem cell differentiation

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] Yang YH, Wang QC, Kong J, Yang JT, Liu JF. Global profiling of lysine lactylation in human lungs.. Proteomics 23(15):e2200437. 2023 Aug. PMID: 37170646.

[4] Cheng Z, Huang H, Li M, Chen Y. Proteomic analysis identifies PFKP lactylation in SW480 colon cancer cells.. iScience 27(1):108645. 2024 Jan 19. PMID: 38155775.

[5] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[6] Bao Q, Wan N, He Z, Cao J, Yuan W et al.. Subcellular Proteomic Mapping of Lysine Lactylation.. J Am Soc Mass Spectrom 35(12):3221-3232. 2024 Dec 4. PMID: 39569522.

[7] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.

[8] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[9] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.