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Overview

Uniprot IDQ8BH95
Protein NameEnoyl-CoA hydratase, mitochondrial
Gene NameEchs1
OrganismMus musculus

Kla Sites from experimental identification

Position Flanking peptide
101 FAAGADIKEMQNRTF
204 RISAQDAKQAGLVSK
282 GMTAFVEKRKANFKD
41 QYIITEKKGKNSSVG
43 IITEKKGKNSSVGLI

Function

Converts unsaturated trans-2-enoyl-CoA species ((2E)-enoyl-CoA) to the corresponding (3S)-3-hydroxyacyl-CoA species through addition of a water molecule to the double bond. Catalyzes the hydration of medium- and short-chained fatty enoyl-CoA thioesters from 4 carbons long (C4) up to C16 (By similarity). Has high substrate specificity for crotonyl-CoA ((2E)-butenoyl-CoA) and moderate specificity for acryloyl-CoA, 3-methylcrotonyl-CoA (3-methyl-(2E)-butenoyl-CoA) and methacrylyl-CoA ((2E)-2-methylpropenoyl-CoA). Can bind tiglyl-CoA (2-methylcrotonoyl-CoA), but hydrates only a small amount of this substrate (By similarity). Plays a key role in the beta-oxidation spiral of short- and medium-chain fatty acid oxidation. At a lower rate than the hydratase reaction, catalyzes the isomerase reaction of trans-3-enoyl-CoA species (such as (3E)-hexenoyl-CoA) to trans-2-enoyl-CoA species (such as (2E)-hexenoyl-CoA), which are subsequently hydrated to 3(S)-3-hydroxyacyl-CoA species (such as (3S)-hydroxyhexanoyl-CoA) (By similarity)

Protein Sequence

10 MAALRALLPR 20 ACSSLLSSVR 30 CPELRRFASG 40 ANFQYIITEK 50 KGKNSSVGLI 60 QLNRPKALNA 70 LCNGLIEELN 80 QALETFEQDP 90 AVGAIVLTGG 100 DKAFAAGADI 110 KEMQNRTFQD 120 CYSSKFLSHW 130 DHITRVKKPV 140 IAAVNGYALG 150 GGCELAMMCD 160 IIYAGEKAQF 170 GQPEILLGTI 180 PGAGGTQRLT 190 RAVGKSLAME 200 MVLTGDRISA 210 QDAKQAGLVS 220 KIFPVEKLVE 230 EAIQCAEKIA 240 SNSKIVVAMA 250 KESVNAAFEM 260 TLTEGNKLEK 270 RLFYSTFATD 280 DRREGMTAFV 290 EKRKANFKDH

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005759 mitochondrial matrix
Cellular Component GO:0005739 mitochondrion
Molecular Function GO:0120092 (2E)-butenoyl-CoA hydratase activity
Molecular Function GO:0043956 3-hydroxypropionyl-CoA dehydratase activity
Molecular Function GO:0004165 delta(3)-delta(2)-enoyl-CoA isomerase activity
Molecular Function GO:0004300 enoyl-CoA hydratase activity
Biological Process GO:0006635 fatty acid beta-oxidation
Biological Process GO:0006574 L-valine catabolic process

Reference

[1] Chang J, Wu W, Qian P, Lu Z, He X et al.. Multi-omics study on the effect of moderate-intensity exercise on protein lactylation in mouse muscle tissue.. Front Cell Dev Biol 12:1472338. 2024. PMID: 39935788.

[2] Zhuo W, Zhang M, Tan J, Gao Y, Wang Y et al.. Lysine lactylation analysis of proteins in the heart of the Kawasaki disease mouse model.. Front Cell Dev Biol 13:1550220. 2025. PMID: 40114965.

[3] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.