Search Results

Overview

Uniprot IDQ8BWT1
Protein Name3-ketoacyl-CoA thiolase, mitochondrial
Gene NameAcaa2
OrganismMus musculus

Kla Sites from experimental identification

Position Flanking peptide
137 RNVRFGTKFGLDLKL
214 EVKTKKGKQTMQVDE
234 TTLEQLQKLPSVFKK
240 QKLPSVFKKDGTVTA
241 KLPSVFKKDGTVTAG
269 IASEDAVKKHNFTPL
270 ASEDAVKKHNFTPLA
340 ALDLDPSKTNVSGGA

Function

In the production of energy from fats, this is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA. Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain unbranched 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms. Also catalyzes the condensation of two acetyl-CoA molecules into acetoacetyl-CoA and could be involved in the production of ketone bodies. Also displays hydrolase activity on various fatty acyl-CoAs (By similarity). Thereby, could be responsible for the production of acetate in a side reaction to beta-oxidation (By similarity). Abolishes BNIP3-mediated apoptosis and mitochondrial damage (By similarity)

Protein Sequence

10 MALLRGVFIV 20 AAKRTPFGAY 30 GGLLKDFSAT 40 DLTEFAARAA 50 LSAGKVPPET 60 IDSVIVGNVM 70 QSSSDAAYLA 80 RHVGLRVGVP 90 TETGALTLNR 100 LCGSGFQSIV 110 SGCQEICSKD 120 AEVVLCGGTE 130 SMSQSPYCVR 140 NVRFGTKFGL 150 DLKLEDTLWA 160 GLTDQHVKLP 170 MGMTAENLAA 180 KYNISREDCD 190 RYALQSQQRW 200 KAANEAGYFN 210 EEMAPIEVKT 220 KKGKQTMQVD 230 EHARPQTTLE 240 QLQKLPSVFK 250 KDGTVTAGNA 260 SGVSDGAGAV 270 IIASEDAVKK 280 HNFTPLARVV 290 GYFVSGCDPT 300 IMGIGPVPAI 310 NGALKKAGLS 320 LKDMDLIDVN 330 EAFAPQFLSV 340 QKALDLDPSK 350 TNVSGGAIAL 360 GHPLGGSGSR 370 ITAHLVHELR 380 RRGGKYAVGS 390 ACIGGGQGIA LIIQNTA

Gene Ontology

Classification GO ID Description
Cellular Component GO:0036064 ciliary basal body
Cellular Component GO:0005743 mitochondrial inner membrane
Cellular Component GO:0005759 mitochondrial matrix
Cellular Component GO:0005739 mitochondrion
Cellular Component GO:0016604 nuclear body
Molecular Function GO:0003985 acetyl-CoA C-acetyltransferase activity
Molecular Function GO:0003988 acetyl-CoA C-acyltransferase activity
Molecular Function GO:0003986 acetyl-CoA hydrolase activity
Molecular Function GO:0047617 fatty acyl-CoA hydrolase activity
Biological Process GO:0006084 acetyl-CoA metabolic process
Biological Process GO:0071456 cellular response to hypoxia
Biological Process GO:0006635 fatty acid beta-oxidation
Biological Process GO:1902109 negative regulation of mitochondrial membrane permeability involved in apoptotic process
Biological Process GO:1901029 negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway

Reference

[1] Chang J, Wu W, Qian P, Lu Z, He X et al.. Multi-omics study on the effect of moderate-intensity exercise on protein lactylation in mouse muscle tissue.. Front Cell Dev Biol 12:1472338. 2024. PMID: 39935788.

[2] Zhuo W, Zhang M, Tan J, Gao Y, Wang Y et al.. Lysine lactylation analysis of proteins in the heart of the Kawasaki disease mouse model.. Front Cell Dev Biol 13:1550220. 2025. PMID: 40114965.

[3] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.