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Overview

Uniprot ID	Q8CHP8
Protein Name	Phosphoglycolate phosphatase
Gene Name	Pgp
Organism	Mus musculus

Kla Sites from experimental identification

Position	Flanking peptide
300	QESDCMFKKKMVPDF

Function

Phosphatase with activity against multiple substrates (PubMed:27294321). Acts as a metabolite repair enzyme which eliminates toxic glycolytic side products (PubMed:27294321). Dephosphorylates 2-phosphoglycolate which is generated during the repair of oxidative DNA lesions (PubMed:27179418, PubMed:27294321, PubMed:27731369). This controls triosephosphate isomerase activity, glycerolipid partitioning and cell proliferation in the embryo and is required for embryonic development (PubMed:27731369). Dephosphorylates 4-phosphoerythronate, a side product which is produced by GAPDH and which inhibits 6-phosphogluconate dehydrogenase (PubMed:27294321). Dephosphorylation of 4-phosphoerythronate prevents the accumulation of high 4-phosphoerythronate levels which would otherwise inhibit the pentose phosphate pathway (By similarity). Dephosphorylates 2-phospho-L-lactate which is a product of a pyruvate kinase side reaction (PubMed:27294321). This prevents the inhibition of fructose-2,6-bisphosphate production and allows glycolysis to occur (By similarity). Has glycerol-3-phosphate phosphatase activity, hydrolyzing glycerol-3-phosphate into glycerol and thereby regulating the cellular levels of glycerol-3-phosphate, a metabolic intermediate of glucose, lipid and energy metabolism (PubMed:26755581). Has been shown to have tyrosine-protein phosphatase activity although the physiological relevance is unclear (PubMed:26755581). In vitro, has phosphatase activity toward ADP, ATP, GDP and GTP (PubMed:24338473)

Protein Sequence

10 MAEAEAGGDE 20 ARCVRLSAER 30 AKLLLAEVDT 40 LLFDCDGVLW 50 RGETAVPGAP 60 ETLRALRARG 70 KRLGFITNNS 80 SKTRTAYAEK 90 LRRLGFGGPV 100 GPEAGLEVFG 110 TAYCSALYLR 120 QRLAGVPDPK 130 AYVLGSPALA 140 AELEAVGVTS 150 VGVGPDVLHG 160 DGPSDWLAVP 170 LEPDVRAVVV 180 GFDPHFSYMK 190 LTKAVRYLQQ 200 PDCLLVGTNM 210 DNRLPLENGR 220 FIAGTGCLVR 230 AVEMAAQRQA 240 DIIGKPSRFI 250 FDCVSQEYGI 260 NPERTVMVGD 270 RLDTDILLGS 280 TCSLKTILTL 290 TGVSSLEDVK 300 SNQESDCMFK 310 KKMVPDFYVD 320 SIADLLPALQ G

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005737	cytoplasm
Molecular Function	GO:0043262	ADP phosphatase activity
Molecular Function	GO:0000287	magnesium ion binding
Molecular Function	GO:0008967	phosphoglycolate phosphatase activity
Molecular Function	GO:0004725	protein tyrosine phosphatase activity
Molecular Function	GO:0000121	sn-glycerol 1-phosphatase activity
Molecular Function	GO:0043136	sn-glycerol 3-phosphatase activity
Biological Process	GO:0006114	glycerol biosynthetic process
Biological Process	GO:0006650	glycerophospholipid metabolic process
Biological Process	GO:0045721	negative regulation of gluconeogenesis
Biological Process	GO:0110052	toxic metabolite repair

Reference

[1] Chang J, Wu W, Qian P, Lu Z, He X et al.. Multi-omics study on the effect of moderate-intensity exercise on protein lactylation in mouse muscle tissue.. Front Cell Dev Biol 12:1472338. 2024. PMID: 39935788.

[2] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.