Overview
| Uniprot ID | Q8IV48 |
|---|
| Protein Name | 3'-5' exoribonuclease 1 |
|---|
| Gene Name | ERI1 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 52 |
GQETKGSKFITSSAS |
Function
RNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication (PubMed:14536070, PubMed:16912046, PubMed:17135487, PubMed:37352860). A 2' and 3'-hydroxyl groups at the last nucleotide of the histone 3'-end is required for efficient 3'-end histone mRNA exonuclease activity and degradation of RNA substrates (PubMed:14536070, PubMed:16912046, PubMed:17135487). Also able to degrade the 3'-overhangs of short interfering RNAs (siRNAs) in vitro, suggesting a possible role as regulator of RNA interference (RNAi) (PubMed:14961122). Required for binding the 5'-ACCCA-3' sequence present in stem-loop structure (PubMed:14536070, PubMed:16912046). Able to bind other mRNAs (PubMed:14536070, PubMed:16912046). Required for 5.8S rRNA 3'-end processing (PubMed:37352860). Also binds to 5.8s ribosomal RNA (By similarity). Binds with high affinity to the stem-loop structure of replication-dependent histone pre-mRNAs (PubMed:14536070, PubMed:16912046, PubMed:17135487). In vitro, does not have sequence specificity (PubMed:17135487). In vitro, has weak DNA exonuclease activity (PubMed:17135487). In vitro, shows biphasic kinetics such that there is rapid hydrolysis of the last three unpaired RNA nucleotides in the 39 flanking sequence followed by a much slower cleavage through the stem that occurs over a longer incubation period in the order of hours (PubMed:17135487). ERI1-mediated RNA metabolism plays a key role in chondrogenesis (PubMed:37352860)
Protein Sequence
10
MEDPQSKEPA
20
GEAVALALLE
30
SPRPEGGEEP
40
PRPSPEETQQ
50
CKFDGQETKG
60
SKFITSSASD
70
FSDPVYKEIA
80
ITNGCINRMS
90
KEELRAKLSE
100
FKLETRGVKD
110
VLKKRLKNYY
120
KKQKLMLKES
130
NFADSYYDYI
140
CIIDFEATCE
150
EGNPPEFVHE
160
IIEFPVVLLN
170
THTLEIEDTF
180
QQYVRPEINT
190
QLSDFCISLT
200
GITQDQVDRA
210
DTFPQVLKKV
220
IDWMKLKELG
230
TKYKYSLLTD
240
GSWDMSKFLN
250
IQCQLSRLKY
260
PPFAKKWINI
270
RKSYGNFYKV
280
PRSQTKLTIM
290
LEKLGMDYDG
300
RPHCGLDDSK
310
NIARIAVRML
320
QDGCELRINE
330
KMHAGQLMSV
340
SSSLPIEGTP
PPQMPHFRK
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0071204 |
histone pre-mRNA 3'end processing complex |
| Cellular Component |
GO:0005730 |
nucleolus |
| Cellular Component |
GO:0005634 |
nucleus |
| Molecular Function |
GO:0008408 |
3'-5' exonuclease activity |
| Molecular Function |
GO:0000175 |
3'-5'-RNA exonuclease activity |
| Molecular Function |
GO:0008859 |
exoribonuclease II activity |
| Molecular Function |
GO:0071207 |
histone pre-mRNA stem-loop binding |
| Molecular Function |
GO:0046872 |
metal ion binding |
| Molecular Function |
GO:0043022 |
ribosome binding |
| Molecular Function |
GO:0019843 |
rRNA binding |
| Biological Process |
GO:0000467 |
exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) |
| Biological Process |
GO:0000460 |
maturation of 5.8S rRNA |
| Biological Process |
GO:0060906 |
negative regulation of regulatory ncRNA-mediated heterochromatin formation |
| Biological Process |
GO:0031047 |
regulatory ncRNA-mediated gene silencing |
| Biological Process |
GO:0031125 |
rRNA 3'-end processing |
Reference
PMID: N/A