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Overview

Uniprot ID	Q8IV63
Protein Name	Serine/threonine-protein kinase VRK3
Gene Name	VRK3
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
141	RQSPQTLKRSRVTTS
197	TCDSGPQKQKFSLKL
382	SLGYCMLKWLYGFLP

Function

Plays a role in the regulation of the cell cycle by phosphorylating the nuclear envelope protein barrier-to-autointegration factor/BAF that is required for disassembly and reassembly, respectively, of the nuclear envelope during mitosis (PubMed:25899223). Under normal physiological conditions, negatively regulates ERK activity along with VHR/DUSP3 phosphatase in the nucleus, causing timely and transient action of ERK. Stress conditions activate CDK5 which phosphorylates VRK3 to increase VHR phosphatase activity and suppress prolonged ERK activation that causes cell death (PubMed:27346674). For example, upon glutamate induction, promotes nuclear localization of HSP70/HSPA1A to inhibit ERK activation via VHR/DUSP3 phosphatase (PubMed:27941812)

Protein Sequence

10 MISFCPDCGK 20 SIQAAFKFCP 30 YCGNSLPVEE 40 HVGSQTFVNP 50 HVSSFQGSKR 60 GLNSSFETSP 70 KKVKWSSTVT 80 SPRLSLFSDG 90 DSSESEDTLS 100 SSERSKGSGS 110 RPPTPKSSPQ 120 KTRKSPQVTR 130 GSPQKTSCSP 140 QKTRQSPQTL 150 KRSRVTTSLE 160 ALPTGTVLTD 170 KSGRQWKLKS 180 FQTRDNQGIL 190 YEAAPTSTLT 200 CDSGPQKQKF 210 SLKLDAKDGR 220 LFNEQNFFQR 230 AAKPLQVNKW 240 KKLYSTPLLA 250 IPTCMGFGVH 260 QDKYRFLVLP 270 SLGRSLQSAL 280 DVSPKHVLSE 290 RSVLQVACRL 300 LDALEFLHEN 310 EYVHGNVTAE 320 NIFVDPEDQS 330 QVTLAGYGFA 340 FRYCPSGKHV 350 AYVEGSRSPH 360 EGDLEFISMD 370 LHKGCGPSRR 380 SDLQSLGYCM 390 LKWLYGFLPW 400 TNCLPNTEDI 410 MKQKQKFVDK 420 PGPFVGPCGH 430 WIRPSETLQK 440 YLKVVMALTY 450 EEKPPYAMLR 460 NNLEALLQDL 470 RVSPYDPIGL PMVP

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005737	cytoplasm
Cellular Component	GO:0005654	nucleoplasm
Cellular Component	GO:0005634	nucleus
Molecular Function	GO:0005524	ATP binding
Molecular Function	GO:0004693	cyclin-dependent protein serine/threonine kinase activity
Molecular Function	GO:0072542	protein phosphatase activator activity
Molecular Function	GO:0019903	protein phosphatase binding
Molecular Function	GO:0004674	protein serine/threonine kinase activity
Biological Process	GO:0006974	DNA damage response
Biological Process	GO:0007165	signal transduction
Biological Process	GO:0007519	skeletal muscle tissue development

Reference

[1] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[2] Cheng Z, Huang H, Li M, Chen Y. Proteomic analysis identifies PFKP lactylation in SW480 colon cancer cells.. iScience 27(1):108645. 2024 Jan 19. PMID: 38155775.