Search Results

Overview

Uniprot IDQ8IVT2
Protein NameMitotic interactor and substrate of PLK1
Gene NameMISP
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
154 IQGQAVRKSSTVATL
540 APALRLQKSQSSDLL

Function

Plays a role in mitotic spindle orientation and mitotic progression. Regulates the distribution of dynactin at the cell cortex in a PLK1-dependent manner, thus stabilizing cortical and astral microtubule attachments required for proper mitotic spindle positioning. May link microtubules to the actin cytospkeleton and focal adhesions. May be required for directed cell migration and centrosome orientation. May also be necessary for proper stacking of the Golgi apparatus

Protein Sequence

10 MDRVTRYPIL 20 GIPQAHRGTG 30 LVLDGDTSYT 40 YHLVCMGPEA 50 SGWGQDEPQT 60 WPTDHRAQQG 70 VQRQGVSYSV 80 HAYTGQPSPR 90 GLHSENREDE 100 GWQVYRLGAR 110 DAHQGRPTWA 120 LRPEDGEDKE 130 MKTYRLDAGD 140 ADPRRLCDLE 150 RERWAVIQGQ 160 AVRKSSTVAT 170 LQGTPDHGDP 180 RTPGPPRSTP 190 LEENVVDREQ 200 IDFLAARQQF 210 LSLEQANKGA 220 PHSSPARGTP 230 AGTTPGASQA 240 PKAFNKPHLA 250 NGHVVPIKPQ 260 VKGVVREENK 270 VRAVPTWASV 280 QVVDDPGSLA 290 SVESPGTPKE 300 TPIEREIRLA 310 QEREADLREQ 320 RGLRQATDHQ 330 ELVEIPTRPL 340 LTKLSLITAP 350 RRERGRPSLY 360 VQRDIVQETQ 370 REEDHRREGL 380 HVGRASTPDW 390 VSEGPQPGLR 400 RALSSDSILS 410 PAPDARAADP 420 APEVRKVNRI 430 PPDAYQPYLS 440 PGTPQLEFSA 450 FGAFGKPSSL 460 STAEAKAATS 470 PKATMSPRHL 480 SESSGKPLST 490 KQEASKPPRG 500 CPQANRGVVR 510 WEYFRLRPLR 520 FRAPDEPQQA 530 QVPHVWGWEV 540 AGAPALRLQK 550 SQSSDLLERE 560 RESVLRREQE 570 VAEERRNALF 580 PEVFSPTPDE 590 NSDQNSRSSS 600 QASGITGSYS 610 VSESPFFSPI 620 HLHSNVAWTV 630 EDPVDSAPPG 640 QRKKEQWYAG 650 INPSDGINSE 660 VLEAIRVTRH 670 KNAMAERWES RIYASEEDD

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005884 actin filament
Cellular Component GO:0005938 cell cortex
Cellular Component GO:0005925 focal adhesion
Cellular Component GO:0005886 plasma membrane
Cellular Component GO:0031616 spindle pole centrosome
Molecular Function GO:0051015 actin filament binding
Biological Process GO:0030953 astral microtubule organization
Biological Process GO:0051301 cell division
Biological Process GO:0016477 cell migration
Biological Process GO:0030866 cortical actin cytoskeleton organization
Biological Process GO:0051660 establishment of centrosome localization
Biological Process GO:0000132 establishment of mitotic spindle orientation
Biological Process GO:0090307 mitotic spindle assembly
Biological Process GO:0051640 organelle localization

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[3] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.