Overview
| Uniprot ID | Q8IXW5 |
|---|
| Protein Name | Putative RNA polymerase II subunit B1 CTD phosphatase RPAP2 |
|---|
| Gene Name | RPAP2 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 125 |
KISTKTNKVYDITER |
| 343 |
HFKRKFAKSNQVSRS |
Function
Protein phosphatase that displays CTD phosphatase activity and regulates transcription of snRNA genes. Recognizes and binds phosphorylated 'Ser-7' of the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and mediates dephosphorylation of 'Ser-5' of the CTD, thereby promoting transcription of snRNA genes (PubMed:17643375, PubMed:22137580, PubMed:24997600). Downstream of EIF2AK3/PERK, dephosphorylates ERN1, a sensor for the endoplasmic reticulum unfolded protein response (UPR), to abort failed ER-stress adaptation and trigger apoptosis (PubMed:30118681)
Protein Sequence
10
MADFAGPSSA
20
GRKAGAPRCS
30
RKAAGTKQTS
40
TLKQEDASKR
50
KAELEAAVRK
60
KIEFERKALH
70
IVEQLLEENI
80
TEEFLMECGR
90
FITPAHYSDV
100
VDERSIVKLC
110
GYPLCQKKLG
120
IVPKQKYKIS
130
TKTNKVYDIT
140
ERKSFCSNFC
150
YQASKFFEAQ
160
IPKTPVWVRE
170
EERHPDFQLL
180
KEEQSGHSGE
190
EVQLCSKAIK
200
TSDIDNPSHF
210
EKQYESSSSS
220
THSDSSSDNE
230
QDFVSSILPG
240
NRPNSTNIRP
250
QLHQKSIMKK
260
KAGHKANSKH
270
KDKEQTVVDV
280
TEQLGDCKLD
290
SQEKDATCEL
300
PLQKVNTQSS
310
SNSTLPERLK
320
ASENSESEYS
330
RSEITLVGIS
340
KKSAEHFKRK
350
FAKSNQVSRS
360
VSSSVQVCPE
370
VGKRNLLKVL
380
KETLIEWKTE
390
ETLRFLYGQN
400
YASVCLKPEA
410
SLVKEELDED
420
DIISDPDSHF
430
PAWRESQNSL
440
DESLPFRGSG
450
TAIKPLPSYE
460
NLKKETEKLN
470
LRIREFYRGR
480
YVLGEETTKS
490
QDSEEHDSTF
500
PLIDSSSQNQ
510
IRKRIVLEKL
520
SKVLPGLLVP
530
LQITLGDIYT
540
QLKNLVRTFR
550
LTNRNIIHKP
560
AEWTLIAMVL
570
LSLLTPILGI
580
QKHSQEGMVF
590
TRFLDTLLEE
600
LHLKNEDLES
610
LTIIFRTSCL
PE
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0005730 |
nucleolus |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0097550 |
transcription preinitiation complex |
| Molecular Function |
GO:0004722 |
protein serine/threonine phosphatase activity |
| Molecular Function |
GO:0043175 |
RNA polymerase core enzyme binding |
| Molecular Function |
GO:0008420 |
RNA polymerase II CTD heptapeptide repeat phosphatase activity |
| Molecular Function |
GO:0008270 |
zinc ion binding |
| Biological Process |
GO:0036499 |
PERK-mediated unfolded protein response |
| Biological Process |
GO:0009301 |
snRNA transcription |
Reference
[1] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.