Search Results

Overview

Uniprot ID	Q8IZ73
Protein Name	Pseudouridylate synthase RPUSD2
Gene Name	RPUSD2
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
100	APGPGKHKKRRGATR
115	ERVVPPPKKRRTGVS
98	AAAPGPGKHKKRRGA

Function

Pseudouridine synthase that catalyzes pseudouridylation of mRNAs (PubMed:31477916, PubMed:35051350). In addition, it catalyzes the formation of pseudouridine mainly at positions 31 and 32 in the anticodon loop of cytosolic and mitochondrial tRNAs, and at position 34 of certain cytosolic tRNAs such as tRNA(Ile) (PubMed:41136621, PubMed:41698914)

Protein Sequence

10 MWLDRRGWLR 20 VLGHWRYDLR 30 RPSFTRTWSG 40 DKGPMAETVS 50 TQVGTEGGLR 60 ASHQQNGDAG 70 GDAKVELSPG 80 PPKPAGREVE 90 PAPVGGEHPS 100 AAAPGPGKHK 110 KRRGATRERV 120 VPPPKKRRTG 130 VSFGDEHFAE 140 TSYYFEGGLR 150 KVRPYYFDFR 160 TYCKGRWVGH 170 SLLHVFSTEF 180 RAQPLAYYEA 190 AVRAGRLQLN 200 EKPVQDLNIV 210 LKDNDFLRNT 220 VHRHEPPVTA 230 EPIRLLAENE 240 DVVVVDKPSS 250 IPVHPCGRFR 260 HNTVIFILGK 270 EHQLKELHPL 280 HRLDRLTSGV 290 LMFAKTAAVS 300 ERIHEQVRDR 310 QLEKEYVCRV 320 EGEFPTEEVT 330 CKEPILVVSY 340 KVGVCRVDPR 350 GKPCETVFQR 360 LSYNGQSSVV 370 RCRPLTGRTH 380 QIRVHLQFLG 390 HPILNDPIYN 400 SVAWGPSRGR 410 GGYIPKTNEE 420 LLRDLVAEHQ 430 AKQSLDVLDL 440 CEGDLSPGLT 450 DSTAPSSELG 460 KDDLEELAAA 470 AQKMEEVAEA 480 APQELDTIAL 490 ASEKAVETDV 500 MNQETDPLCA 510 ECRLVRQDPL 520 PQDLVMFLHA 530 LRYKGPGFEY 540 FSPMPAWAQD DWQKD

Gene Ontology

Classification	GO ID	Description
Molecular Function	GO:0009982	pseudouridine synthase activity
Molecular Function	GO:0003723	RNA binding
Biological Process	GO:0000455	enzyme-directed rRNA pseudouridine synthesis
Biological Process	GO:0006397	mRNA processing
Biological Process	GO:1990481	mRNA pseudouridine synthesis

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[3] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.