Search Results

Overview

Uniprot IDQ8K215
Protein NameLYR motif-containing protein 4
Gene NameLyrm4
OrganismMus musculus

Kla Sites from experimental identification

Position Flanking peptide
47 FRENKNVKDPVEIQA

Function

Stabilizing factor, of the core iron-sulfur cluster (ISC) assembly complex, that regulates, in association with NDUFAB1, the stability and the cysteine desulfurase activity of NFS1 and participates in the [2Fe-2S] clusters assembly on the scaffolding protein ISCU (By similarity). The core iron-sulfur cluster (ISC) assembly complex is involved in the de novo synthesis of a [2Fe-2S] cluster, the first step of the mitochondrial iron-sulfur protein biogenesis. This process is initiated by the cysteine desulfurase complex (NFS1:LYRM4:NDUFAB1) that produces persulfide which is delivered on the scaffold protein ISCU in a FXN-dependent manner. Then this complex is stabilized by FDX2 which provides reducing equivalents to accomplish the [2Fe-2S] cluster assembly. Finally, the [2Fe-2S] cluster is transferred from ISCU to chaperone proteins, including HSCB, HSPA9 and GLRX5 (By similarity). May also participates in the iron-sulfur protein biogenesis in the cytoplasm through its interaction with the cytoplasmic form of NFS1 (By similarity)

Protein Sequence

10 MAASSRAQVL 20 DLYRAMMRES 30 KHFSAYNYRM 40 YAVRRIRDAF 50 RENKNVKDPV 60 EIQALVNKAK 70 RDLEIIRRQV 80 HIGQLYSTDK 90 LIIENQEKPR T

Gene Ontology

Classification GO ID Description
Cellular Component GO:1990221 L-cysteine desulfurase complex
Cellular Component GO:0099128 mitochondrial [2Fe-2S] assembly complex
Cellular Component GO:0005739 mitochondrion
Cellular Component GO:0016604 nuclear body
Cellular Component GO:0005634 nucleus
Molecular Function GO:0060090 molecular adaptor activity
Molecular Function GO:0042803 protein homodimerization activity
Molecular Function GO:0005198 structural molecule activity
Biological Process GO:0044571 [2Fe-2S] cluster assembly
Biological Process GO:0044572 [4Fe-4S] cluster assembly
Biological Process GO:0016226 iron-sulfur cluster assembly

Reference

[1] Zhuo W, Zhang M, Tan J, Gao Y, Wang Y et al.. Lysine lactylation analysis of proteins in the heart of the Kawasaki disease mouse model.. Front Cell Dev Biol 13:1550220. 2025. PMID: 40114965.

[2] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.