Overview
| Uniprot ID | Q8K2C6 |
|---|
| Protein Name | NAD-dependent protein deacylase sirtuin-5, mitochondrial |
|---|
| Gene Name | Sirt5 |
|---|
| Organism | Mus musculus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 203 |
DARIPVDKLPRCEEA |
Function
NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins (PubMed:21908771, PubMed:22076378, PubMed:23806337, PubMed:24315375, PubMed:24703693). Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation and deglutarylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting (PubMed:19410549, PubMed:24703693). Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species (By similarity). Activates SHMT2 by mediating its desuccinylation (By similarity). Modulates ketogenesis through the desuccinylation and activation of HMGCS2 (PubMed:24315375). Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro such as Uox (PubMed:23085393)
Protein Sequence
10
MRPLLIAPGR
20
FISQLCCRRK
30
PPASPQSKIC
40
LTMARPSSNM
50
ADFRKCFANA
60
KHIAIISGAG
70
VSAESGVPTF
80
RGAGGYWRKW
90
QAQDLATPQA
100
FARNPSQVWE
110
FYHYRREVMR
120
SKEPNPGHLA
130
IAQCEARLRD
140
QGRRVVVITQ
150
NIDELHRKAG
160
TKNLLEIHGT
170
LFKTRCTSCG
180
TVAENYRSPI
190
CPALAGKGAP
200
EPETQDARIP
210
VDKLPRCEEA
220
GCGGLLRPHV
230
VWFGENLDPA
240
ILEEVDRELA
250
LCDLCLVVGT
260
SSVVYPAAMF
270
APQVASRGVP
280
VAEFNMETTP
290
ATDRFRFHFP
300
GPCGKTLPEA
310
LAPHETERTS
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005758 |
mitochondrial intermembrane space |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0005743 |
mitochondrial inner membrane |
| Cellular Component |
GO:0005759 |
mitochondrial matrix |
| Cellular Component |
GO:0005739 |
mitochondrion |
| Cellular Component |
GO:0005634 |
nucleus |
| Molecular Function |
GO:0017136 |
histone deacetylase activity, NAD-dependent |
| Molecular Function |
GO:0070403 |
NAD+ binding |
| Molecular Function |
GO:0061697 |
protein-glutaryllysine deglutarylase activity |
| Molecular Function |
GO:0036054 |
protein-malonyllysine demalonylase activity |
| Molecular Function |
GO:0036055 |
protein-succinyllysine desuccinylase activity |
| Molecular Function |
GO:0008270 |
zinc ion binding |
| Biological Process |
GO:0010667 |
negative regulation of cardiac muscle cell apoptotic process |
| Biological Process |
GO:2000378 |
negative regulation of reactive oxygen species metabolic process |
| Biological Process |
GO:0036047 |
peptidyl-lysine demalonylation |
| Biological Process |
GO:0036049 |
peptidyl-lysine desuccinylation |
| Biological Process |
GO:0006476 |
protein deacetylation |
| Biological Process |
GO:0061698 |
protein deglutarylation |
| Biological Process |
GO:0036046 |
protein demalonylation |
| Biological Process |
GO:0036048 |
protein desuccinylation |
| Biological Process |
GO:0010566 |
regulation of ketone biosynthetic process |
| Biological Process |
GO:0002931 |
response to ischemia |
| Biological Process |
GO:0031667 |
response to nutrient levels |
Reference
[1] Zhuo W, Zhang M, Tan J, Gao Y, Wang Y et al.. Lysine lactylation analysis of proteins in the heart of the Kawasaki disease mouse model.. Front Cell Dev Biol 13:1550220. 2025. PMID: 40114965.