Overview
| Uniprot ID | Q8MIZ3 |
|---|
| Protein Name | m7GpppX diphosphatase |
|---|
| Gene Name | DCPS |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 138 |
VVYPATEKHLQKYLH |
Function
Decapping scavenger enzyme that catalyzes the cleavage of a residual cap structure following the degradation of mRNAs by the 3'->5' exosome-mediated mRNA decay pathway. Hydrolyzes cap analog structures like 7-methylguanosine nucleoside triphosphate (m7GpppG) with up to 10 nucleotide substrates (small capped oligoribonucleotides) and specifically releases 5'-phosphorylated RNA fragments and 7-methylguanosine monophosphate (m7GMP). Cleaves cap analog structures like tri-methyl guanosine nucleoside triphosphate (m3(2,2,7)GpppG) with very poor efficiency. Does not hydrolyze unmethylated cap analog (GpppG) and shows no decapping activity on intact m7GpppG-capped mRNA molecules longer than 25 nucleotides. Does not hydrolyze 7-methylguanosine diphosphate (m7GDP) to m7GMP. May also play a role in the 5'->3 mRNA decay pathway; m7GDP, the downstream product released by the 5'->3' mRNA mediated decapping activity, may be also converted by DCPS to m7GMP. Binds to m7GpppG and strongly to m7GDP. Plays a role in first intron splicing of pre-mRNAs. Inhibits activation-induced cell death
Protein Sequence
10
MADTAPQPSK
20
RKRERDPEEA
30
EAPSTEEKEA
40
RVGNGTSAPV
50
RLPFSGFRVK
60
KVLRESARDK
70
IIFLHGKVNE
80
ASGDGDGEDA
90
IVILEKTPFQ
100
VDQVAQLLMG
110
SPELQLQFSN
120
DIYSTYHLFP
130
PRQLSDVKTT
140
VVYPATEKHL
150
QKYLHQDLHL
160
VRETGGDYKN
170
ITLPHLESQS
180
LSIQWVYNIL
190
DKKAEADRIV
200
FENPDPSDGF
210
VLIPDLKWNQ
220
KQLDDLYLIA
230
ICHRRGIKSL
240
RDLTPEHLPL
250
LRNILREGQE
260
AILQRYQVTG
270
DRLRVYLHYL
280
PSYYHLHVHF
290
TALGFEAPGA
300
GVERAHLLAE
310
VIENLEQDPE
320
HYQRRTLTFA
330
LRADDPLLTL
LQEAQRS
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0000932 |
P-body |
| Cellular Component |
GO:0048471 |
perinuclear region of cytoplasm |
| Molecular Function |
GO:0140932 |
5'-(N(7)-methyl 5'-triphosphoguanosine)-[mRNA] diphosphatase activity |
| Molecular Function |
GO:0042802 |
identical protein binding |
| Molecular Function |
GO:0000340 |
RNA 7-methylguanosine cap binding |
| Biological Process |
GO:0000290 |
deadenylation-dependent decapping of nuclear-transcribed mRNA |
| Biological Process |
GO:0045292 |
mRNA cis splicing, via spliceosome |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.