Search Results

Overview

Uniprot IDQ8N201
Protein NameIntegrator complex subunit 1
Gene NameINTS1
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
100 AEAAVAEKRAISPSI
76 SALTGLTKRPKLSST
79 TGLTKRPKLSSTPPL

Function

Component of the integrator complex, a multiprotein complex that terminates RNA polymerase II (Pol II) transcription in the promoter-proximal region of genes (PubMed:25201415, PubMed:33243860, PubMed:38570683). The integrator complex provides a quality checkpoint during transcription elongation by driving premature transcription termination of transcripts that are unfavorably configured for transcriptional elongation: the complex terminates transcription by (1) catalyzing dephosphorylation of the C-terminal domain (CTD) of Pol II subunit POLR2A/RPB1 and SUPT5H/SPT5, (2) degrading the exiting nascent RNA transcript via endonuclease activity and (3) promoting the release of Pol II from bound DNA (PubMed:33243860). The integrator complex is also involved in terminating the synthesis of non-coding Pol II transcripts, such as enhancer RNAs (eRNAs), small nuclear RNAs (snRNAs), telomerase RNAs and long non-coding RNAs (lncRNAs) (PubMed:16239144, PubMed:26308897, PubMed:30737432). Within the integrator complex, INTS1 is involved in the post-termination step: INTS1 displaces INTS3 and the SOSS factors, allowing the integrator complex to return to the closed conformation, ready to bind to the paused elongation complex for another termination cycle (PubMed:38570683). Mediates recruitment of cytoplasmic dynein to the nuclear envelope, probably as component of the integrator complex (PubMed:23904267)

Protein Sequence

10 MNRAKPTTVR 20 RPSAAAKPSG 30 HPPPGDFIAL 40 GSKGQANESK 50 TASTLLKPAP 60 SGLPSERKRD 70 AAAALSSASA 80 LTGLTKRPKL 90 SSTPPLSALG 100 RLAEAAVAEK 110 RAISPSIKEP 120 SVVPIEVLPT 130 VLLDEIEAAE 140 LEGNDDRIEG 150 VLCGAVKQLK 160 VTRAKPDSTL 170 YLSLMYLAKI 180 KPNIFATEGV 190 IEALCSLLRR 200 DASINFKAKG 210 NSLVSVLACN 220 LLMAAYEEDE 230 NWPEIFVKVY 240 IEDSLGERIW 250 VDSPHCKTFV 260 DNIQTAFNTR 270 MPPRSVLLQG 280 EAGRVAGDLG 290 AGSSPHPSLT 300 EEEDSQTELL 310 IAEEKLSPEQ 320 EGQLMPRYEE 330 LAESVEEYVL 340 DMLRDQLNRR 350 QPIDNVSRNL 360 LRLLTSTCGY 370 KEVRLLAVQK 380 LEMWLQNPKL 390 TRPAQDLLMS 400 VCMNCNTHGS 410 EDMDVISHLI 420 KIRLKPKVLL 430 NHFMLCIREL 440 LSAHKDNLGT 450 TIKLVIFNEL 460 SSARNPNNMQ 470 VLYTALQHSS 480 ELAPKFLAMV 490 FQDLLTNKDD 500 YLRASRALLR 510 EIIKQTKHEI 520 NFQAFCLGLM 530 QERKEPQYLE 540 MEFKERFVVH 550 ITDVLAVSMM 560 LGITAQVKEA 570 GIAWDKGEKR 580 NLEVLRSFQN 590 QIAAIQRDAV 600 WWLHTVVPSI 610 SKLAPKDYVH 620 CLHKVLFTEQ 630 PETYYKWDNW 640 PPESDRNFFL 650 RLCSEVPILE 660 DTLMRILVIG 670 LSRELPLGPA 680 DAMELADHLV 690 KRAAAVQADD 700 VEVLKVGRTQ 710 LIDAVLNLCT 720 YHHPENIQLP 730 PGYQPPNLAI 740 STLYWKAWPL 750 LLVVAAFNPE 760 NIGLAAWEEY 770 PTLKMLMEMV 780 MTNNYSYPPC 790 TLTDEETRTE 800 MLNRELQTAQ 810 REKQEILAFE 820 GHLAAASTKQ 830 TITESSSLLL 840 SQLTSLDPQG 850 PPRRPPPHIL 860 DQVKSLNQSL 870 RLGHLLCRSR 880 NPDFLLHIIQ 890 RQASSQSMPW 900 LADLVQSSEG 910 SLDVLPVQCL 920 CEFLLHDAVD 930 DAASGEEDDE 940 GESKEQKAKK 950 RQRQQKQRQL 960 LGRLQDLLLG 970 PKADEQTTCE 980 VLDYFLRRLG 990 SSQVASRVLA 1000 MKGLSLVLSE 1010 GSLRDGEEKE 1020 PPMEEDVGDT 1030 DVLQGYQWLL 1040 RDLPRLPLFD 1050 SVRSTTALAL 1060 QQAIHMETDP 1070 QTISAYLIYL 1080 SQHTPVEEQA 1090 QHSDLALDVA 1100 RLVVERSTIM 1110 SHLFSKLSPS 1120 AASDAVLSAL 1130 LSIFSRYVRR 1140 MRQSKEGEEV 1150 YSWSESQDQV 1160 FLRWSSGETA 1170 TMHILVVHAM 1180 VILLTLGPPR 1190 ADDSEFQALL 1200 DIWFPEEKPL 1210 PTAFLVDTSE 1220 EALLLPDWLK 1230 LRMIRSEVLR 1240 LVDAALQDLE 1250 PQQLLLFVQS 1260 FGIPVSSMSK 1270 LLQFLDQAVA 1280 HDPQTLEQNI 1290 MDKNYMAHLV 1300 EVQHERGASG 1310 GQTFHSLLTA 1320 SLPPRRDSTE 1330 APKPKSSPEQ 1340 PIGQGRIRVG 1350 TQLRVLGPED 1360 DLAGMFLQIF 1370 PLSPDPRWQS 1380 SSPRPVALAL 1390 QQALGQELAR 1400 VVQGSPEVPG 1410 ITVRVLQALA 1420 TLLSSPHGGA 1430 LVMSMHRSHF 1440 LACPLLRQLC 1450 QYQRCVPQDT 1460 GFSSLFLKVL 1470 LQMLQWLDSP 1480 GVEGGPLRAQ 1490 LRMLASQASA 1500 GRRLSDVRGG 1510 LLRLAEALAF 1520 RQDLEVVSST 1530 VRAVIATLRS 1540 GEQCSVEPDL 1550 ISKVLQGLIE 1560 VRSPHLEELL 1570 TAFFSATADA 1580 ASPFPACKPV 1590 VVVSSLLLQE 1600 EEPLAGGKPG 1610 ADGGSLEAVR 1620 LGPSSGLLVD 1630 WLEMLDPEVV 1640 SSCPDLQLRL 1650 LFSRRKGKGQ 1660 AQVPSFRPYL 1670 LTLFTHQSSW 1680 PTLHQCIRVL 1690 LGKSREQRFD 1700 PSASLDFLWA 1710 CIHVPRIWQG 1720 RDQRTPQKRR 1730 EELVLRVQGP 1740 ELISLVELIL 1750 AEAETRSQDG 1760 DTAACSLIQA 1770 RLPLLLSCCC 1780 GDDESVRKVT 1790 EHLSGCIQQW 1800 GDSVLGRRCR 1810 DLLLQLYLQR 1820 PELRVPVPEV 1830 LLHSEGAASS 1840 SVCKLDGLIH 1850 RFITLLADTS 1860 DSRALENRGA 1870 DASMACRKLA 1880 VAHPLLLLRH 1890 LPMIAALLHG 1900 RTHLNFQEFR 1910 QQNHLSCFLH 1920 VLGLLELLQP 1930 HVFRSEHQGA 1940 LWDCLLSFIR 1950 LLLNYRKSSR 1960 HLAAFINKFV 1970 QFIHKYITYN 1980 APAAISFLQK 1990 HADPLHDLSF 2000 DNSDLVMLKS 2010 LLAGLSLPSR 2020 DDRTDRGLDE 2030 EGEEESSAGS 2040 LPLVSVSLFT 2050 PLTAAEMAPY 2060 MKRLSRGQTV 2070 EDLLEVLSDI 2080 DEMSRRRPEI 2090 LSFFSTNLQR 2100 LMSSAEECCR 2110 NLAFSLALRS 2120 MQNSPSIAAA 2130 FLPTFMYCLG 2140 SQDFEVVQTA 2150 LRNLPEYALL 2160 CQEHAAVLLH 2170 RAFLVGMYGQ 2180 MDPSAQISEA 2190 LRILHMEAVM

Gene Ontology

Classification GO ID Description
Cellular Component GO:0160232 INTAC complex
Cellular Component GO:0032039 integrator complex
Cellular Component GO:0016020 membrane
Cellular Component GO:0031965 nuclear membrane
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Biological Process GO:0034243 regulation of transcription elongation by RNA polymerase II
Biological Process GO:0160240 RNA polymerase II transcription initiation surveillance
Biological Process GO:0016180 snRNA processing
Biological Process GO:0034474 U2 snRNA 3'-end processing

Reference

[1] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[2] Bao Q, Wan N, He Z, Cao J, Yuan W et al.. Subcellular Proteomic Mapping of Lysine Lactylation.. J Am Soc Mass Spectrom 35(12):3221-3232. 2024 Dec 4. PMID: 39569522.

[3] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.