Overview
| Uniprot ID | Q8N2W9 |
|---|
| Protein Name | E3 SUMO-protein ligase PIAS4 |
|---|
| Gene Name | PIAS4 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 125 |
GLGRLPAKTLKPEVR |
| 504 |
KRRCPFQKGLVPAC* |
Function
Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor (PubMed:12511558, PubMed:12631292, PubMed:12727872, PubMed:15831457, PubMed:15976810, PubMed:22508508, PubMed:32832608). Mediates sumoylation of ALKBH5, AXIN1, CEBPA, KLF8, GATA2, PARK7, HERC2, MYB, TCF4 and RNF168 (PubMed:12223491, PubMed:12511558, PubMed:12631292, PubMed:12727872, PubMed:12750312, PubMed:15831457, PubMed:15976810, PubMed:16617055, PubMed:22508508, PubMed:34048572). Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53/TP53 pathway, the Wnt pathway and the steroid hormone signaling pathway (PubMed:11388671). Involved in gene silencing (PubMed:11248056). In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations (PubMed:12727872, PubMed:15831457). Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation (PubMed:21965678). Binds to AT-rich DNA sequences, known as matrix or scaffold attachment regions (MARs/SARs) (By similarity). Catalyzes conjugation of SUMO2 to KAT5 in response to DNA damage, facilitating repair of DNA double-strand breaks (DSBs) via homologous recombination (HR) (PubMed:32832608). Mediates sumoylation of PARP1 in response to PARP1 trapping to chromatin (PubMed:35013556). Mediates sumoylation of KLF8, repressing KLF8 transcriptional activity and cell cycle progression into G(1) phase (PubMed:16617055). Sumoylates ALKBH5 downstream of MAPK8/JNK1 and MAPK9/JNK2 in response to reactive oxygen species (ROS), inhibiting ALKBH5 RNA demethylase activity (PubMed:34048572)
Protein Sequence
10
MAAELVEAKN
20
MVMSFRVSDL
30
QMLLGFVGRS
40
KSGLKHELVT
50
RALQLVQFDC
60
SPELFKKIKE
70
LYETRYAKKN
80
SEPAPQPHRP
90
LDPLTMHSTY
100
DRAGAVPRTP
110
LAGPNIDYPV
120
LYGKYLNGLG
130
RLPAKTLKPE
140
VRLVKLPFFN
150
MLDELLKPTE
160
LVPQNNEKLQ
170
ESPCIFALTP
180
RQVELIRNSR
190
ELQPGVKAVQ
200
VVLRICYSDT
210
SCPQEDQYPP
220
NIAVKVNHSY
230
CSVPGYYPSN
240
KPGVEPKRPC
250
RPINLTHLMY
260
LSSATNRITV
270
TWGNYGKSYS
280
VALYLVRQLT
290
SSELLQRLKT
300
IGVKHPELCK
310
ALVKEKLRLD
320
PDSEIATTGV
330
RVSLICPLVK
340
MRLSVPCRAE
350
TCAHLQCFDA
360
VFYLQMNEKK
370
PTWMCPVCDK
380
PAPYDQLIID
390
GLLSKILSEC
400
EDADEIEYLV
410
DGSWCPIRAE
420
KERSCSPQGA
430
ILVLGPSDAN
440
GLLPAPSVNG
450
SGALGSTGGG
460
GPVGSMENGK
470
PGADVVDLTL
480
DSSSSSEDEE
490
EEEEEEEDED
500
EEGPRPKRRC
510
PFQKGLVPAC
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0000785 |
chromatin |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0016363 |
nuclear matrix |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0016605 |
PML body |
| Cellular Component |
GO:1990234 |
transferase complex |
| Molecular Function |
GO:0003677 |
DNA binding |
| Molecular Function |
GO:0061665 |
SUMO ligase activity |
| Molecular Function |
GO:0019789 |
SUMO transferase activity |
| Molecular Function |
GO:0003712 |
transcription coregulator activity |
| Molecular Function |
GO:0003714 |
transcription corepressor activity |
| Molecular Function |
GO:0061630 |
ubiquitin protein ligase activity |
| Molecular Function |
GO:0031625 |
ubiquitin protein ligase binding |
| Molecular Function |
GO:0008270 |
zinc ion binding |
| Biological Process |
GO:0006351 |
DNA-templated transcription |
| Biological Process |
GO:0006302 |
double-strand break repair |
| Biological Process |
GO:0043124 |
negative regulation of canonical NF-kappaB signal transduction |
| Biological Process |
GO:0045892 |
negative regulation of DNA-templated transcription |
| Biological Process |
GO:0120186 |
negative regulation of protein localization to chromatin |
| Biological Process |
GO:1905168 |
positive regulation of double-strand break repair via homologous recombination |
| Biological Process |
GO:1902174 |
positive regulation of keratinocyte apoptotic process |
| Biological Process |
GO:0033235 |
positive regulation of protein sumoylation |
| Biological Process |
GO:0016925 |
protein sumoylation |
| Biological Process |
GO:0043488 |
regulation of mRNA stability |
| Biological Process |
GO:0006357 |
regulation of transcription by RNA polymerase II |
| Biological Process |
GO:0042359 |
vitamin D metabolic process |
| Biological Process |
GO:0016055 |
Wnt signaling pathway |
Reference
PMID: N/A