Search Results

Overview

Uniprot ID	Q8N3C0
Protein Name	Activating signal cointegrator 1 complex subunit 3
Gene Name	ASCC3
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
1983	KKWKPIMKGPHARGR
236	KYLNSTLKEMTEVPR
60	FLNEKLEKSKMQSIN
62	NEKLEKSKMQSINED

Function

ATPase involved both in DNA repair and rescue of stalled ribosomes (PubMed:22055184, PubMed:28757607, PubMed:32099016, PubMed:32579943, PubMed:36302773). 3'-5' DNA helicase involved in repair of alkylated DNA: promotes DNA unwinding to generate single-stranded substrate needed for ALKBH3, enabling ALKBH3 to process alkylated N3-methylcytosine (3mC) within double-stranded regions (PubMed:22055184). Also involved in activation of the ribosome quality control (RQC) pathway, a pathway that degrades nascent peptide chains during problematic translation (PubMed:28757607, PubMed:32099016, PubMed:32579943, PubMed:36302773). Drives the splitting of stalled ribosomes that are ubiquitinated in a ZNF598-dependent manner, as part of the ribosome quality control trigger (RQT) complex (PubMed:28757607, PubMed:32099016, PubMed:32579943, PubMed:36302773). Part of the ASC-1 complex that enhances NF-kappa-B, SRF and AP1 transactivation (PubMed:12077347)

Protein Sequence

10 MALPRLTGAL 20 RSFSNVTKQD 30 NYNEEVADLK 40 IKRSKLHEQV 50 LDLGLTWKKI 60 IKFLNEKLEK 70 SKMQSINEDL 80 KDILHAAKQI 90 VGTDNGREAI 100 ESGAAFLFMT 110 FHLKDSVGHK 120 ETKAIKQMFG 130 PFPSSSATAA 140 CNATNRIISH 150 FSQDDLTALV 160 QMTEKEHGDR 170 VFFGKNLAFS 180 FDMHDLDHFD 190 ELPINGETQK 200 TISLDYKKFL 210 NEHLQEACTP 220 ELKPVEKTNG 230 SFLWCEVEKY 240 LNSTLKEMTE 250 VPRVEDLCCT 260 LYDMLASIKS 270 GDELQDELFE 280 LLGPEGLELI 290 EKLLQNRITI 300 VDRFLNSSND 310 HRFQALQDNC 320 KKILGENAKP 330 NYGCQVTIQS 340 EQEKQLMKQY 350 RREEKRIARR 360 EKKAGEDLEV 370 SEGLMCFDPK 380 ELRIQREQAL 390 LNARSVPILS 400 RQRDADVEKI 410 HYPHVYDSQA 420 EAMKTSAFIA 430 GAKMILPEGI 440 QRENNKLYEE 450 VRIPYSEPMP 460 LSFEEKPVYI 470 QDLDEIGQLA 480 FKGMKRLNRI 490 QSIVFETAYN 500 TNENMLICAP 510 TGAGKTNIAM 520 LTVLHEIRQH 530 FQQGVIKKNE 540 FKIVYVAPMK 550 ALAAEMTDYF 560 SRRLEPLGII 570 VKELTGDMQL 580 SKSEILRTQM 590 LVTTPEKWDV 600 VTRKSVGDVA 610 LSQIVRLLIL 620 DEVHLLHEDR 630 GPVLESIVAR 640 TLRQVESTQS 650 MIRILGLSAT 660 LPNYLDVATF 670 LHVNPYIGLF 680 FFDGRFRPVP 690 LGQTFLGIKC 700 ANKMQQLNNM 710 DEVCYENVLK 720 QVKAGHQVMV 730 FVHARNATVR 740 TAMSLIERAK 750 NCGHIPFFFP 760 TQGHDYVLAE 770 KQVQRSRNKQ 780 VRELFPDGFS 790 IHHAGMLRQD 800 RNLVENLFSN 810 GHIKVLVCTA 820 TLAWGVNLPA 830 HAVIIKGTQI 840 YAAKRGSFVD 850 LGILDVMQIF 860 GRAGRPQFDK 870 FGEGIIITTH 880 DKLSHYLTLL 890 TQRNPIESQF 900 LESLADNLNA 910 EIALGTVTNV 920 EEAVKWISYT 930 YLYVRMRANP 940 LAYGISHKAY 950 QIDPTLRKHR 960 EQLVIEVGRK 970 LDKAQMIRFE 980 ERTGYFSSTD 990 LGRTASHYYI 1000 KYNTIETFNE 1010 LFDAHKTEGD 1020 IFAIVSKAEE 1030 FDQIKVREEE 1040 IEELDTLLSN 1050 FCELSTPGGV 1060 ENSYGKINIL 1070 LQTYISRGEM 1080 DSFSLISDSA 1090 YVAQNAARIV 1100 RALFEIALRK 1110 RWPTMTYRLL 1120 NLSKVIDKRL 1130 WGWASPLRQF 1140 SILPPHILTR 1150 LEEKKLTVDK 1160 LKDMRKDEIG 1170 HILHHVNIGL 1180 KVKQCVHQIP 1190 SVMMEASIQP 1200 ITRTVLRVTL 1210 SIYADFTWND 1220 QVHGTVGEPW 1230 WIWVEDPTND 1240 HIYHSEYFLA 1250 LKKQVISKEA 1260 QLLVFTIPIF 1270 EPLPSQYYIR 1280 AVSDRWLGAE 1290 AVCIINFQHL 1300 ILPERHPPHT 1310 ELLDLQPLPI 1320 TALGCKAYEA 1330 LYNFSHFNPV 1340 QTQIFHTLYH 1350 TDCNVLLGAP 1360 TGSGKTVAAE 1370 LAIFRVFNKY 1380 PTSKAVYIAP 1390 LKALVRERMD 1400 DWKVRIEEKL 1410 GKKVIELTGD 1420 VTPDMKSIAK 1430 ADLIVTTPEK 1440 WDGVSRSWQN 1450 RNYVQQVTIL 1460 IIDEIHLLGE 1470 ERGPVLEVIV 1480 SRTNFISSHT 1490 EKPVRIVGLS 1500 TALANARDLA 1510 DWLNIKQMGL 1520 FNFRPSVRPV 1530 PLEVHIQGFP 1540 GQHYCPRMAS 1550 MNKPAFQAIR 1560 SHSPAKPVLI 1570 FVSSRRQTRL 1580 TALELIAFLA 1590 TEEDPKQWLN 1600 MDEREMENII 1610 ATVRDSNLKL 1620 TLAFGIGMHH 1630 AGLHERDRKT 1640 VEELFVNCKV 1650 QVLIATSTLA 1660 WGVNFPAHLV 1670 IIKGTEYYDG 1680 KTRRYVDFPI 1690 TDVLQMMGRA 1700 GRPQFDDQGK 1710 AVILVHDIKK 1720 DFYKKFLYEP 1730 FPVESSLLGV 1740 LSDHLNAEIA 1750 GGTITSKQDA 1760 LDYITWTYFF 1770 RRLIMNPSYY 1780 NLGDVSHDSV 1790 NKFLSHLIEK 1800 SLIELELSYC 1810 IEIGEDNRSI 1820 EPLTYGRIAS 1830 YYYLKHQTVK 1840 MFKDRLKPEC 1850 STEELLSILS 1860 DAEEYTDLPV 1870 RHNEDHMNSE 1880 LAKCLPIESN 1890 PHSFDSPHTK 1900 AHLLLQAHLS 1910 RAMLPCPDYD 1920 TDTKTVLDQA 1930 LRVCQAMLDV 1940 AANQGWLVTV 1950 LNITNLIQMV 1960 IQGRWLKDSS 1970 LLTLPNIENH 1980 HLHLFKKWKP 1990 IMKGPHARGR 2000 TSIESLPELI 2010 HACGGKDHVF 2020 SSMVESELHA 2030 AKTKQAWNFL 2040 SHLPVINVGI 2050 SVKGSWDDLV 2060 EGHNELSVST 2070 LTADKRDDNK 2080 WIKLHADQEY 2090 VLQVSLQRVH 2100 FGFHKGKPES 2110 CAVTPRFPKS 2120 KDEGWFLILG 2130 EVDKRELIAL 2140 KRVGYIRNHH 2150 VASLSFYTPE 2160 IPGRYIYTLY 2170 FMSDCYLGLD 2180 QQYDIYLNVT 2190 QASLSAQVNT 2200 KVSDSLTDLA LK

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0022626	cytosolic ribosome
Cellular Component	GO:0016020	membrane
Cellular Component	GO:0016607	nuclear speck
Cellular Component	GO:0005654	nucleoplasm
Cellular Component	GO:0005634	nucleus
Cellular Component	GO:0180022	RQC-trigger complex
Molecular Function	GO:0043138	3'-5' DNA helicase activity
Molecular Function	GO:0005524	ATP binding
Molecular Function	GO:0016887	ATP hydrolysis activity
Molecular Function	GO:0003723	RNA binding
Biological Process	GO:0006307	DNA alkylation repair
Biological Process	GO:0006260	DNA replication
Biological Process	GO:0072344	rescue of stalled cytosolic ribosome
Biological Process	GO:0032790	ribosome disassembly
Biological Process	GO:1990116	ribosome-associated ubiquitin-dependent protein catabolic process

Reference

[1] Cheng Z, Huang H, Li M, Chen Y. Proteomic analysis identifies PFKP lactylation in SW480 colon cancer cells.. iScience 27(1):108645. 2024 Jan 19. PMID: 38155775.

[2] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.