Search Results

Overview

Uniprot ID	Q8N4T8
Protein Name	3-oxoacyl-[acyl-carrier-protein] reductase
Gene Name	CBR4
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
164	GFSRALAKEVARKKI
190	TDMTKDLKEEHLKKN
195	DLKEEHLKKNIPLGR
196	LKEEHLKKNIPLGRF
59	AFSCDVAKEHDVQNT

Function

Component of the heterotetramer complex KAR (3-ketoacyl-[acyl carrier protein] reductase or 3-ketoacyl-[ACP] reductase) that forms part of the mitochondrial fatty acid synthase (mtFAS). Beta-subunit of the KAR heterotetramer complex, responsible for the 3-ketoacyl-ACP reductase activity of the mtFAS, reduces 3-oxoacyl-[ACP] to (3R)-hydroxyacyl-[ACP] in a NADPH-dependent manner with no chain length preference, thereby participating in mitochondrial fatty acid biosynthesis (PubMed:25203508). The homotetramer has NADPH-dependent quinone reductase activity (in vitro), hence could play a role in protection against cytotoxicity of exogenous quinones (PubMed:19000905). As a heterotetramer, it can also reduce 9,10-phenanthrenequinone, 1,4-benzoquinone and various other o-quinones and p-quinones (in vitro) (PubMed:19000905, PubMed:19571038, PubMed:25203508)

Protein Sequence

10 MDKVCAVFGG 20 SRGIGRAVAQ 30 LMARKGYRLA 40 VIARNLEGAK 50 AAAGDLGGDH 60 LAFSCDVAKE 70 HDVQNTFEEL 80 EKHLGRVNFL 90 VNAAGINRDG 100 LLVRTKTEDM 110 VSQLHTNLLG 120 SMLTCKAAMR 130 TMIQQQGGSI 140 VNVGSIVGLK 150 GNSGQSVYSA 160 SKGGLVGFSR 170 ALAKEVARKK 180 IRVNVVAPGF 190 VHTDMTKDLK 200 EEHLKKNIPL 210 GRFGETIEVA 220 HAVVFLLESP 230 YITGHVLVVD GGLQLIL

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005759	mitochondrial matrix
Biological Process	GO:0051289	protein homotetramerization
Cellular Component	GO:0005739	mitochondrion
Cellular Component	GO:1990204	oxidoreductase complex
Molecular Function	GO:0004316	3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity
Molecular Function	GO:0003955	NAD(P)H dehydrogenase (quinone) activity
Molecular Function	GO:0070402	NADPH binding
Molecular Function	GO:0008753	NADPH dehydrogenase (quinone) activity
Molecular Function	GO:0016616	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
Molecular Function	GO:0048038	quinone binding
Biological Process	GO:0044597	daunorubicin metabolic process
Biological Process	GO:0044598	doxorubicin metabolic process
Biological Process	GO:0006633	fatty acid biosynthetic process
Biological Process	GO:0046949	fatty-acyl-CoA biosynthetic process
Biological Process	GO:0051290	protein heterotetramerization

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[3] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.