Search Results

Overview

Uniprot ID	Q8N5F7
Protein Name	NF-kappa-B-activating protein
Gene Name	NKAP
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
396	ERRKRENKILASFRE

Function

Acts as a transcriptional repressor (PubMed:14550261, PubMed:19409814, PubMed:31587868). Plays a role as a transcriptional corepressor of the Notch-mediated signaling required for T-cell development (PubMed:19409814). Also involved in the TNF and IL-1 induced NF-kappa-B activation. Associates with chromatin at the Notch-regulated SKP2 promoter

Protein Sequence

10 MAPVSGSRSP 20 DREASGSGGR 30 RRSSSKSPKP 40 SKSARSPRGR 50 RSRSHSCSRS 60 GDRNGLTHQL 70 GGLSQGSRNQ 80 SYRSRSRSRS 90 RERPSAPRGI 100 PFASASSSVY 110 YGSYSRPYGS 120 DKPWPSLLDK 130 EREESLRQKR 140 LSERERIGEL 150 GAPEVWGLSP 160 KNPEPDSDEH 170 TPVEDEEPKK 180 STTSASTSEE 190 EKKKKSSRSK 200 ERSKKRRKKK 210 SSKRKHKKYS 220 EDSDSDSDSE 230 TDSSDEDNKR 240 RAKKAKKKEK 250 KKKHRSKKYK 260 KKRSKKSRKE 270 SSDSSSKESQ 280 EEFLENPWKD 290 RTKAEEPSDL 300 IGPEAPKTLT 310 SQDDKPLNYG 320 HALLPGEGAA 330 MAEYVKAGKR 340 IPRRGEIGLT 350 SEEIASFECS 360 GYVMSGSRHR 370 RMEAVRLRKE 380 NQIYSADEKR 390 ALASFNQEER 400 RKRENKILAS 410 FREMVYRKTK GKDDK

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005654	nucleoplasm
Cellular Component	GO:0005634	nucleus
Molecular Function	GO:0003682	chromatin binding
Molecular Function	GO:0031490	chromatin DNA binding
Molecular Function	GO:0003723	RNA binding
Biological Process	GO:0006351	DNA-templated transcription
Biological Process	GO:0000398	mRNA splicing, via spliceosome
Biological Process	GO:0045892	negative regulation of DNA-templated transcription
Biological Process	GO:0000122	negative regulation of transcription by RNA polymerase II
Biological Process	GO:0007219	Notch signaling pathway
Biological Process	GO:0046638	positive regulation of alpha-beta T cell differentiation

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Bao Q, Wan N, He Z, Cao J, Yuan W et al.. Subcellular Proteomic Mapping of Lysine Lactylation.. J Am Soc Mass Spectrom 35(12):3221-3232. 2024 Dec 4. PMID: 39569522.

[3] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.