Overview
| Uniprot ID | Q8N9B5 |
|---|
| Protein Name | Junction-mediating and -regulatory protein |
|---|
| Gene Name | JMY |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 486 |
KLQYAVSKETLQMMR |
Function
Acts both as a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics depending on conditions (PubMed:30420355). In nucleus, acts as a cofactor that increases p53/TP53 response via its interaction with p300/EP300. Increases p53/TP53-dependent transcription and apoptosis, suggesting an important role in p53/TP53 stress response such as DNA damage. In cytoplasm, acts as a nucleation-promoting factor for both branched and unbranched actin filaments (PubMed:30420355). Activates the Arp2/3 complex to induce branched actin filament networks. Also catalyzes actin polymerization in the absence of Arp2/3, creating unbranched filaments (PubMed:30420355). Contributes to cell motility by controlling actin dynamics. May promote the rapid formation of a branched actin network by first nucleating new mother filaments and then activating Arp2/3 to branch off these filaments. Upon nutrient stress, directly recruited by MAP1LC3B to the phagophore membrane surfaces to promote actin assembly during autophagy (PubMed:30420355). The p53/TP53-cofactor and actin activator activities are regulated via its subcellular location (By similarity)
Protein Sequence
10
MSFALEETLE
20
SDWVAVRPHV
30
FDEREKHKFV
40
FIVAWNEIEG
50
KFAITCHNRT
60
AQRQRSGSRE
70
QAGARGGAEA
80
GGAASDGSRG
90
PGSPAGRGRP
100
EATASATLVR
110
SPGPRRSSAW
120
AEGGSPRSTR
130
SLLGDPRLRS
140
PGSKGAESRL
150
RSPVRAKPIP
160
GQKTSEADDA
170
AGAAAAAARP
180
APREAQVSSV
190
RIVSASGTVS
200
EEIEVLEMVK
210
EDEAPLALSD
220
AEQPPPATEL
230
ESPAEECSWA
240
GLFSFQDLRA
250
VHQQLCSVNS
260
QLEPCLPVFP
270
EEPSGMWTVL
280
FGGAPEMTEQ
290
EIDTLCYQLQ
300
VYLGHGLDTC
310
GWKILSQVLF
320
TETDDPEEYY
330
ESLSELRQKG
340
YEEVLQRARK
350
RIQELLDKHK
360
NTESMVELLD
370
LYQMEDEAYS
380
SLAEATTELY
390
QYLLQPFRDM
400
RELAMLRRQQ
410
IKISMENDYL
420
GPRRIESLQK
430
EDADWQRKAH
440
MAVLSIQDLT
450
VKYFEITAKA
460
QKAVYDRMRA
470
DQKKFGKASW
480
AAAAERMEKL
490
QYAVSKETLQ
500
MMRAKEICLE
510
QRKHALKEEM
520
QSLRGGTEAI
530
ARLDQLEADY
540
YDLQLQLYEV
550
QFEILKCEEL
560
LLTAQLESIK
570
RLISEKRDEV
580
VYYDTYESME
590
AMLEKEEMAA
600
SAYLQREELQ
610
KLQQKARQLE
620
ARRGRVSAKK
630
SYLRNKKEIC
640
IAKHNEKIQQ
650
RTRIEDEYRT
660
HHTVQLKREK
670
LHDEEERKSA
680
WVSQERQRTL
690
DRLRTFKQRY
700
PGQVILKSTR
710
LRLAHARRKG
720
AASPVLQEDH
730
CDSLPSVLQV
740
EEKTEEVGEG
750
RVKRGPSQTT
760
EPQSLVQLED
770
TSLTQLEATS
780
LPLSGVTSEL
790
PPTISLPLLN
800
NNLEPCSVTI
810
NPLPSPLPPT
820
PPPPPPPPPP
830
PPPPPLPVAK
840
DSGPETLEKD
850
LPRKEGNEKR
860
IPKSASAPSA
870
HLFDSSQLVS
880
ARKKLRKTAE
890
GLQRRRVSSP
900
MDEVLASLKR
910
GSFHLKKVEQ
920
RTLPPFPDED
930
DSNNILAQIR
940
KGVKLKKVQK
950
DVLRESFTLL
960
PDTDPLTRSI
970
HEALRRIKEA
980
SPESEDEEEA
LPCTDWEN
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0000421 |
autophagosome membrane |
| Cellular Component |
GO:0031252 |
cell leading edge |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0031410 |
cytoplasmic vesicle |
| Cellular Component |
GO:0005856 |
cytoskeleton |
| Cellular Component |
GO:0012505 |
endomembrane system |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Molecular Function |
GO:0003779 |
actin binding |
| Molecular Function |
GO:0071933 |
Arp2/3 complex binding |
| Molecular Function |
GO:0008017 |
microtubule binding |
| Molecular Function |
GO:0003713 |
transcription coactivator activity |
| Biological Process |
GO:0070060 |
'de novo' actin filament nucleation |
| Biological Process |
GO:0070358 |
actin polymerization-dependent cell motility |
| Biological Process |
GO:0034314 |
Arp2/3 complex-mediated actin nucleation |
| Biological Process |
GO:0009267 |
cellular response to starvation |
| Biological Process |
GO:0006281 |
DNA repair |
| Biological Process |
GO:0072332 |
intrinsic apoptotic signaling pathway by p53 class mediator |
| Biological Process |
GO:0043065 |
positive regulation of apoptotic process |
| Biological Process |
GO:0006357 |
regulation of transcription by RNA polymerase II |
Reference
[1] Cheng Z, Huang H, Li M, Chen Y. Proteomic analysis identifies PFKP lactylation in SW480 colon cancer cells.. iScience 27(1):108645. 2024 Jan 19. PMID: 38155775.