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Overview

Uniprot IDQ8NB78
Protein NameLysine-specific histone demethylase 2
Gene NameKDM1B
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
11 PRGRTKKKASFDHSP
33 SGRQAKKKATETTDE

Function

Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and di-methylated 'Lys-4' of histone H3. Has no effect on tri-methylated 'Lys-4', mono-, di- or tri-methylated 'Lys-9', mono-, di- or tri-methylated 'Lys-27', mono-, di- or tri-methylated 'Lys-36' of histone H3, or on mono-, di- or tri-methylated 'Lys-20' of histone H4. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of GLYR1 to achieve such activity, they form a multifunctional enzyme complex that modifies transcribed chromatin and facilitates Pol II transcription through nucleosomes (PubMed:30970244)

Protein Sequence

10 MATPRGRTKK 20 KASFDHSPDS 30 LPLRSSGRQA 40 KKKATETTDE 50 DEDGGSEKKY 60 RKCEKAGCTA 70 TCPVCFASAS 80 ERCAKNGYTS 90 RWYHLSCGEH 100 FCNECFDHYY 110 RSHKDGYDKY 120 TTWKKIWTSN 130 GKTEPSPKAF 140 MADQQLPYWV 150 QCTKPECRKW 160 RQLTKEIQLT 170 PQIAKTYRCG 180 MKPNTAIKPE 190 TSDHCSLPED 200 LRVLEVSNHW 210 WYSMLILPPL 220 LKDSVAAPLL 230 SAYYPDCVGM 240 SPSCTSTNRA 250 AATGNASPGK 260 LEHSKAALSV 270 HVPGMNRYFQ 280 PFYQPNECGK 290 ALCVRPDVME 300 LDELYEFPEY 310 SRDPTMYLAL 320 RNLILALWYT 330 NCKEALTPQK 340 CIPHIIVRGL 350 VRIRCVQEVE 360 RILYFMTRKG 370 LINTGVLSVG 380 ADQYLLPKDY 390 HNKSVIIIGA 400 GPAGLAAARQ 410 LHNFGIKVTV 420 LEAKDRIGGR 430 VWDDKSFKGV 440 TVGRGAQIVN 450 GCINNPVALM 460 CEQLGISMHK 470 FGERCDLIQE 480 GGRITDPTID 490 KRMDFHFNAL 500 LDVVSEWRKD 510 KTQLQDVPLG 520 EKIEEIYKAF 530 IKESGIQFSE 540 LEGQVLQFHL 550 SNLEYACGSN 560 LHQVSARSWD 570 HNEFFAQFAG 580 DHTLLTPGYS 590 VIIEKLAEGL 600 DIQLKSPVQC 610 IDYSGDEVQV 620 TTTDGTGYSA 630 QKVLVTVPLA 640 LLQKGAIQFN 650 PPLSEKKMKA 660 INSLGAGIIE 670 KIALQFPYRF 680 WDSKVQGADF 690 FGHVPPSASK 700 RGLFAVFYDM 710 DPQKKHSVLM 720 SVIAGEAVAS 730 VRTLDDKQVL 740 QQCMATLREL 750 FKEQEVPDPT 760 KYFVTRWSTD 770 PWIQMAYSFV 780 KTGGSGEAYD 790 IIAEDIQGTV 800 FFAGEATNRH 810 FPQTVTGAYL 820 SGVREASKIA AF

Gene Ontology

Classification GO ID Description
Cellular Component GO:0000785 chromatin
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0000786 nucleosome
Cellular Component GO:0005634 nucleus
Molecular Function GO:0071949 FAD binding
Molecular Function GO:0140682 FAD-dependent H3K4me/H3K4me3 demethylase activity
Molecular Function GO:0042393 histone binding
Molecular Function GO:0032452 histone demethylase activity
Molecular Function GO:0016491 oxidoreductase activity
Molecular Function GO:0008270 zinc ion binding
Biological Process GO:0006351 DNA-templated transcription
Biological Process GO:0071514 genomic imprinting
Biological Process GO:0045815 transcription initiation-coupled chromatin remodeling

Reference

[1] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.