Overview
| Uniprot ID | Q8NCN4 |
|---|
| Protein Name | E3 ubiquitin-protein ligase RNF169 |
|---|
| Gene Name | RNF169 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 282 |
SLAFLAGKLNSKVER |
| 286 |
LAGKLNSKVERSQSC |
| 347 |
APDLTIEKRLPFSSL |
| 408 |
VLSPLIIKSTPRNLN |
| 433 |
ASPRILKKWEQIFQE |
Function
Probable E3 ubiquitin-protein ligase that acts as a regulator of double-strand breaks (DSBs) repair following DNA damage. Functions in a non-canonical fashion to harness RNF168-mediated protein recruitment to DSB-containing chromatin, thereby contributing to regulation of DSB repair pathway utilization (PubMed:22492721, PubMed:30773093). Once recruited to DSB repair sites by recognizing and binding ubiquitin catalyzed by RNF168, competes with TP53BP1 and BRCA1 for association with RNF168-modified chromatin, thereby favouring homologous recombination repair (HRR) and single-strand annealing (SSA) instead of non-homologous end joining (NHEJ) mediated by TP53BP1 (PubMed:30104380, PubMed:30773093). E3 ubiquitin-protein ligase activity is not required for regulation of DSBs repair
Protein Sequence
10
MAAAGPSTRA
20
SSAAAAAALS
30
RRGRRGRCDE
40
TAAAKTGAPG
50
PASGPSLLVL
60
SPPLLQPPLP
70
PRPEESGCAG
80
CLEPPGEAAA
90
LPCGHSLCRG
100
CAQRAADAAG
110
PGCPRCRARG
120
PGWARRRARD
130
DGQADSEVLG
140
ECARRSQPER
150
CRPRRDGGAA
160
AAGPRPEQEP
170
RAAPAEPDFI
180
FRAPIKLSKP
190
GELREEYESL
200
RKLREEKLQE
210
EKPSEDQIHK
220
LLPEDTETGK
230
RKMDEQKKRD
240
EPLVLKTNLE
250
RCPARLSDSE
260
NEEPSRGQMT
270
QTHRSAFVSK
280
NNSYSLAFLA
290
GKLNSKVERS
300
QSCSDTAQER
310
AKSRVRAVPG
320
NKAKVTTMTP
330
ASNPIIGVLL
340
STQNNRCVSA
350
PDLTIEKRLP
360
FSSLSSLASL
370
HKPERSVSPE
380
SNDSISEELN
390
HFKPIVCSPC
400
TPPKRLPDGR
410
VLSPLIIKST
420
PRNLNRSLQK
430
QTSYEASPRI
440
LKKWEQIFQE
450
RQIKKTLSKA
460
TLTSLAPEMG
470
EELLGSEGIH
480
SSKEKPLVAV
490
NTRLSGGQVL
500
SEYTGPTSAD
510
LDHFPSVSQT
520
KAEQDSDNKS
530
STEIPLETCC
540
SSELKGGGSG
550
TSLEREQFEG
560
LGSTPDAKLD
570
KTCISRAMKI
580
TTVNSVLPQN
590
SVLGGVLKTK
600
QQLKTLNHFD
610
LTNGVLVESL
620
SEEPLPSLRR
630
GRKRHCKTKH
640
LEQNGSLKKL
650
RQTSGEVGLA
660
PTDPVLREME
670
QKLQQEEEDR
680
QLALQLQRMF
690
DNERRTVSRR
700
KGSVDQYLLR
SSNMAGAK
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0016604 |
nuclear body |
| Cellular Component |
GO:0005730 |
nucleolus |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0035861 |
site of double-strand break |
| Molecular Function |
GO:0070530 |
K63-linked polyubiquitin modification-dependent protein binding |
| Molecular Function |
GO:0031491 |
nucleosome binding |
| Molecular Function |
GO:0061630 |
ubiquitin protein ligase activity |
| Molecular Function |
GO:0061649 |
ubiquitin-modified histone reader activity |
| Molecular Function |
GO:0004842 |
ubiquitin-protein transferase activity |
| Molecular Function |
GO:0008270 |
zinc ion binding |
| Biological Process |
GO:0006974 |
DNA damage response |
| Biological Process |
GO:0006302 |
double-strand break repair |
| Biological Process |
GO:0000724 |
double-strand break repair via homologous recombination |
| Biological Process |
GO:2000780 |
negative regulation of double-strand break repair |
| Biological Process |
GO:0016567 |
protein ubiquitination |
Reference
PMID: N/A