Overview
| Uniprot ID | Q8NDF8 |
|---|
| Protein Name | Terminal nucleotidyltransferase 4B |
|---|
| Gene Name | TENT4B |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 512 |
NTSNSTNKSQHGSAR |
| 526 |
RLFRSSSKGFQGTTQ |
| 557 |
NNQYYHGKKRKHKRD |
Function
Terminal nucleotidyltransferase that catalyzes preferentially the transfer of ATP and GTP on RNA 3' poly(A) tail creating a heterogeneous 3' poly(A) tail leading to mRNAs stabilization by protecting mRNAs from active deadenylation (PubMed:21788334, PubMed:30026317). Also functions as a catalytic subunit of a TRAMP-like complex which has a poly(A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism. Polyadenylation with short oligo(A) tails is required for the degradative activity of the exosome on several of its nuclear RNA substrates. Doesn't need a cofactor for polyadenylation activity (in vitro) (PubMed:21788334, PubMed:21855801). Required for cytoplasmic polyadenylation of mRNAs involved in carbohydrate metabolism, including the glucose transporter SLC2A1/GLUT1 (PubMed:28383716). Plays a role in replication-dependent histone mRNA degradation, probably through terminal uridylation of mature histone mRNAs. May play a role in sister chromatid cohesion (PubMed:18172165). Mediates 3' adenylation of the microRNA MIR21 followed by its 3'-to-5' trimming by the exoribonuclease PARN leading to degradation (PubMed:25049417). Mediates 3' adenylation of H/ACA box snoRNAs (small nucleolar RNAs) followed by its 3'-to-5' trimming by the exoribonuclease PARN which enhances snoRNA stability and maturation (PubMed:22442037)
Protein Sequence
10
MYRSGERLLG
20
SHALPAEQRD
30
FLPLETTNNN
40
NNHHQPGAWA
50
RRAGSSASSP
60
PSASSSPHPS
70
AAVPAADPAD
80
SASGSSNKRK
90
RDNKASGGRA
100
AGGGRADGGG
110
VVYSGTPWKR
120
RNYNQGVVGL
130
HEEISDFYEY
140
MSPRPEEEKM
150
RMEVVNRIES
160
VIKELWPSAD
170
VQIFGSFKTG
180
LYLPTSDIDL
190
VVFGKWENLP
200
LWTLEEALRK
210
HKVADEDSVK
220
VLDKATVPII
230
KLTDSFTEVK
240
VDISFNVQNG
250
VRAADLIKDF
260
TKKYPVLPYL
270
VLVLKQFLLQ
280
RDLNEVFTGG
290
IGSYSLFLMA
300
VSFLQLHPRE
310
DACIPNTNYG
320
VLLIEFFELY
330
GRHFNYLKTG
340
IRIKDGGSYV
350
AKDEVQKNML
360
DGYRPSMLYI
370
EDPLQPGNDV
380
GRSSYGAMQV
390
KQAFDYAYVV
400
LSHAVSPIAK
410
YYPNNETESI
420
LGRIIRVTDE
430
VATYRDWISK
440
QWGLKNRPEP
450
SCNGPVSSSS
460
ATQSSSSDVD
470
SDATPCKTPK
480
QLLCRPSTGN
490
RVGSQDVSLE
500
SSQAVGKMQS
510
TQTTNTSNST
520
NKSQHGSARL
530
FRSSSKGFQG
540
TTQTSHGSLM
550
TNKQHQGKSN
560
NQYYHGKKRK
570
HKRDAPLSDL
CR
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0005730 |
nucleolus |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0031499 |
TRAMP complex |
| Molecular Function |
GO:0070566 |
adenylyltransferase activity |
| Molecular Function |
GO:0005524 |
ATP binding |
| Molecular Function |
GO:0070568 |
guanylyltransferase activity |
| Molecular Function |
GO:0046872 |
metal ion binding |
| Molecular Function |
GO:1990817 |
poly(A) RNA polymerase activity |
| Molecular Function |
GO:0003723 |
RNA binding |
| Molecular Function |
GO:0070034 |
telomerase RNA binding |
| Biological Process |
GO:0033500 |
carbohydrate homeostasis |
| Biological Process |
GO:0051301 |
cell division |
| Biological Process |
GO:0071044 |
histone mRNA catabolic process |
| Biological Process |
GO:0010587 |
miRNA catabolic process |
| Biological Process |
GO:0031124 |
mRNA 3'-end processing |
| Biological Process |
GO:0060212 |
negative regulation of nuclear-transcribed mRNA poly(A) tail shortening |
| Biological Process |
GO:0032211 |
negative regulation of telomere maintenance via telomerase |
| Biological Process |
GO:0071051 |
poly(A)-dependent snoRNA 3'-end processing |
| Biological Process |
GO:0043634 |
polyadenylation-dependent ncRNA catabolic process |
| Biological Process |
GO:1905870 |
positive regulation of 3'-UTR-mediated mRNA stabilization |
| Biological Process |
GO:0031123 |
RNA 3'-end processing |
| Biological Process |
GO:0006401 |
RNA catabolic process |
| Biological Process |
GO:0006364 |
rRNA processing |
| Cellular Component |
GO:0005829 |
cytosol |
Reference
[1] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.