Overview
| Uniprot ID | Q8NG08 |
|---|
| Protein Name | DNA helicase B |
|---|
| Gene Name | HELB |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 216 |
MTALQFPKIMEFLPV |
Function
5'-3' DNA helicase involved in DNA damage response by acting as an inhibitor of DNA end resection (PubMed:25617833, PubMed:26774285). Recruitment to single-stranded DNA (ssDNA) following DNA damage leads to inhibit the nucleases catalyzing resection, such as EXO1, BLM and DNA2, possibly via the 5'-3' ssDNA translocase activity of HELB (PubMed:26774285). As cells approach S phase, DNA end resection is promoted by the nuclear export of HELB following phosphorylation (PubMed:26774285). Acts independently of TP53BP1 (PubMed:26774285). Unwinds duplex DNA with 5'-3' polarity. Has single-strand DNA-dependent ATPase and DNA helicase activities. Prefers ATP and dATP as substrates (PubMed:12181327). During S phase, may facilitate cellular recovery from replication stress (PubMed:22194613)
Protein Sequence
10
MARSSPYLRQ
20
LQGPLLPPRD
30
LVEEDDDYLN
40
DDVEEDEESV
50
FIDAEELCSG
60
GVKAGSLPGC
70
LRVSICDENT
80
QETCKVFGRF
90
PITGAWWRVK
100
VQVKPVVGSR
110
SYQYQVQGFP
120
SYFLQSDMSP
130
PNQKHICALF
140
LKECEVSSDD
150
VNKFLTWVKE
160
VSNYKNLNFE
170
NLRETLRTFH
180
KETGRKDQKQ
190
PTQNGQEELF
200
LDNEMSLPLE
210
NTIPFRNVMT
220
ALQFPKIMEF
230
LPVLLPRHFK
240
WIIGSGSKEM
250
LKEIEEILGT
260
HPWKLGFSKI
270
TYREWKLLRC
280
EASWIAFCQC
290
ESLLQLMTDL
300
EKNALIMYSR
310
LKQICREDGH
320
TYVEVNDLTL
330
TLSNHMSFHA
340
ASESLKFLKD
350
IGVVTYEKSC
360
VFPYDLYHAE
370
RAIAFSICDL
380
MKKPPWHLCV
390
DVEKVLASIH
400
TTKPENSSDD
410
ALNESKPDEV
420
RLENPVDVVD
430
TQDNGDHIWT
440
NGENEINAEI
450
SEVQLDQDQV
460
EVPLDRDQVA
470
ALEMICSNPV
480
TVISGKGGCG
490
KTTIVSRLFK
500
HIEQLEEREV
510
KKACEDFEQD
520
QNASEEWITF
530
TEQSQLEADK
540
AIEVLLTAPT
550
GKAAGLLRQK
560
TGLHAYTLCQ
570
VNYSFYSWTQ
580
TMMTTNKPWK
590
FSSVRVLVVD
600
EGSLVSVGIF
610
KSVLNLLCEH
620
SKLSKLIILG
630
DIRQLPSIEP
640
GNLLKDLFET
650
LKSRNCAIEL
660
KTNHRAESQL
670
IVDNATRISR
680
RQFPKFDAEL
690
NISDNPTLPI
700
SIQDKTFIFV
710
RLPEEDASSQ
720
SSKTNHHSCL
730
YSAVKTLLQE
740
NNLQNAKTSQ
750
FIAFRRQDCD
760
LINDCCCKHY
770
TGHLTKDHQS
780
RLVFGIGDKI
790
CCTRNAYLSD
800
LLPENISGSQ
810
QNNDLDASSE
820
DFSGTLPDFA
830
KNKRDFESNV
840
RLCNGEIFFI
850
TNDVTDVTFG
860
KRRSLTINNM
870
AGLEVTVDFK
880
KLMKYCRIKH
890
AWARTIHTFQ
900
GSEEQTVVYV
910
VGKAGRQHWQ
920
HVYTAVTRGR
930
CRVYVIAEES
940
QLRNAIMKNS
950
FPRKTRLKHF
960
LQSKLSSSGA
970
PPADFPSPRK
980
SSGDSGGPST
990
PSASPLPVVT
1000
DHAMTNDVTW
1010
SEASSPDERT
1020
LTFAERWQLS
1030
SPDGVDTDDD
1040
LPKSRASKRT
1050
CGVNDDESPS
1060
KIFMVGESPQ
1070
VSSRLQNLRL
1080
NNLIPRQLFK
PTDNQET
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0016604 |
nuclear body |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0035861 |
site of double-strand break |
| Molecular Function |
GO:0043139 |
5'-3' DNA helicase activity |
| Molecular Function |
GO:0005524 |
ATP binding |
| Molecular Function |
GO:0016887 |
ATP hydrolysis activity |
| Molecular Function |
GO:0044877 |
protein-containing complex binding |
| Molecular Function |
GO:0017116 |
single-stranded DNA helicase activity |
| Biological Process |
GO:0006974 |
DNA damage response |
| Biological Process |
GO:0006281 |
DNA repair |
| Biological Process |
GO:0006260 |
DNA replication |
| Biological Process |
GO:0006269 |
DNA replication, synthesis of primer |
| Biological Process |
GO:0006261 |
DNA-templated DNA replication |
| Biological Process |
GO:2000042 |
negative regulation of double-strand break repair via homologous recombination |
| Biological Process |
GO:1903775 |
regulation of DNA double-strand break processing |
Reference
[1] Cheng Z, Huang H, Li M, Chen Y. Proteomic analysis identifies PFKP lactylation in SW480 colon cancer cells.. iScience 27(1):108645. 2024 Jan 19. PMID: 38155775.