Search Results

Overview

Uniprot IDQ8NG31
Protein NameOuter kinetochore KNL1 complex subunit KNL1
Gene NameKNL1
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
1087 LNEPLSSKSQRRKSL
1221 KFGIGKGKNLGVSFP
1481 DQPPLPKKGQSSINK
393 NKTVFKSKQNTAFQD
75 FQTESHMKIVRKSEM
841 CGKSPIEKSGVLKSN
861 LEDESVQKPKFPKEK
872 PKEKQNVKIWGRKSV
886 VGGPKIDKTIVFSED

Function

Acts as a component of the outer kinetochore KNL1 complex that serves as a docking point for spindle assembly checkpoint components and mediates microtubule-kinetochore interactions (PubMed:15502821, PubMed:17981135, PubMed:18045986, PubMed:19893618, PubMed:21199919, PubMed:22000412, PubMed:22331848, PubMed:27881301, PubMed:30100357). Kinetochores, consisting of a centromere-associated inner segment and a microtubule-contacting outer segment, play a crucial role in chromosome segregation by mediating the physical connection between centromeric DNA and spindle microtubules (PubMed:18045986, PubMed:19893618, PubMed:27881301). The outer kinetochore is made up of the ten-subunit KMN network, comprising the MIS12, NDC80 and KNL1 complexes, and auxiliary microtubule-associated components; together they connect the outer kinetochore with the inner kinetochore, bind microtubules, and mediate interactions with mitotic checkpoint proteins that delay anaphase until chromosomes are bioriented on the spindle (PubMed:17981135, PubMed:19893618, PubMed:22000412, PubMed:38459127, PubMed:38459128). Required for kinetochore binding by a distinct subset of kMAPs (kinetochore-bound microtubule-associated proteins) and motors (PubMed:19893618). Acts in coordination with CENPK to recruit the NDC80 complex to the outer kinetochore (PubMed:18045986, PubMed:27881301). Can bind either to microtubules or to the protein phosphatase 1 (PP1) catalytic subunits PPP1CA and PPP1CC (via overlapping binding sites), it has higher affinity for PP1 (PubMed:30100357). Recruits MAD2L1 to the kinetochore and also directly links BUB1 and BUB1B to the kinetochore (PubMed:17981135, PubMed:19893618, PubMed:22000412, PubMed:22331848, PubMed:25308863). In addition to orienting mitotic chromosomes, it is also essential for alignment of homologous chromosomes during meiotic metaphase I (By similarity). In meiosis I, required to activate the spindle assembly checkpoint at unattached kinetochores to correct erroneous kinetochore-microtubule attachments (By similarity)

Protein Sequence

10 MDGVSSEANE 20 ENDNIERPVR 30 RRHSSILKPP 40 RSPLQDLRGG 50 NERVQESNAL 60 RNKKNSRRVS 70 FADTIKVFQT 80 ESHMKIVRKS 90 EMEGCSAMVP 100 SQLQLLPPGF 110 KRFSCLSLPE 120 TETGENLLLI 130 QNKKLEDNYC 140 EITGMNTLLS 150 APIHTQMQQK 160 EFSIIEHTRE 170 RKHANDQTVI 180 FSDENQMDLT 190 SSHTVMITKG 200 LLDNPISEKS 210 TKIDTTSFLA 220 NLKLHTEDSR 230 MKKEVNFSVD 240 QNTSSENKID 250 FNDFIKRLKT 260 GKCSAFPDVP 270 DKENFEIPIY 280 SKEPNSASST 290 HQMHVSLKED 300 ENNSNITRLF 310 REKDDGMNFT 320 QCHTANIQTL 330 IPTSSETNSR 340 ESKGNDITIY 350 GNDFMDLTFN 360 HTLQILPATG 370 NFSEIENQTQ 380 NAMDVTTGYG 390 TKASGNKTVF 400 KSKQNTAFQD 410 LSINSADKIH 420 ITRSHIMGAE 430 THIVSQTCNQ 440 DARILAMTPE 450 SIYSNPSIQG 460 CKTVFYSSCN 470 DAMEMTKCLS 480 NMREEKNLLK 490 HDSNYAKMYC 500 NPDAMSSLTE 510 KTIYSGEENM 520 DITKSHTVAI 530 DNQIFKQDQS 540 NVQIAAAPTP 550 EKEMMLQNLM 560 TTSEDGKMNV 570 NCNSVPHVSK 580 ERIQQSLSNP 590 LSISLTDRKT 600 ELLSGENMDL 610 TESHTSNLGS 620 QVPLAAYNLA 630 PESTSESHSQ 640 SKSSSDECEE 650 ITKSRNEPFQ 660 RSDIIAKNSL 670 TDTWNKDKDW 680 VLKILPYLDK 690 DSPQSADCNQ 700 EIATSHNIVY 710 CGGVLDKQIT 720 NRNTVSWEQS 730 LFSTTKPLFS 740 SGQFSMKNHD 750 TAISSHTVKS 760 VLGQNSKLAE 770 PLRKSLSNPT 780 PDYCHDKMII 790 CSEEEQNMDL 800 TKSHTVVIGF 810 GPSELQELGK 820 TNLEHTTGQL 830 TTMNRQIAVK 840 VEKCGKSPIE 850 KSGVLKSNCI 860 MDVLEDESVQ 870 KPKFPKEKQN 880 VKIWGRKSVG 890 GPKIDKTIVF 900 SEDDKNDMDI 910 TKSYTIEINH 920 RPLLEKRDCH 930 LVPLAGTSET 940 ILYTCRQDDM 950 EITRSHTTAL 960 ECKTVSPDEI 970 TTRPMDKTVV 980 FVDNHVELEM 990 TESHTVFIDY 1000 QEKERTDRPN 1010 FELSQRKSLG 1020 TPTVICTPTE 1030 ESVFFPGNGE 1040 SDRLVANDSQ 1050 LTPLEEWSNN 1060 RGPVEVADNM 1070 ELSKSATCKN 1080 IKDVQSPGFL 1090 NEPLSSKSQR 1100 RKSLKLKNDK 1110 TIVFSENHKN 1120 DMDITQSCMV 1130 EIDNESALED 1140 KEDFHLAGAS 1150 KTILYSCGQD 1160 DMEITRSHTT 1170 ALECKTLLPN 1180 EIAIRPMDKT 1190 VLFTDNYSDL 1200 EVTDSHTVFI 1210 DCQATEKILE 1220 ENPKFGIGKG 1230 KNLGVSFPKD 1240 NSCVQEIAEK 1250 QALAVGNKIV 1260 LHTEQKQQLF 1270 AATNRTTNEI 1280 IKFHSAAMDE 1290 KVIGKVVDQA 1300 CTLEKAQVES 1310 CQLNNRDRRN 1320 VDFTSSHATA 1330 VCGSSDNYSC 1340 LPNVISCTDN 1350 LEGSAMLLCD 1360 KDEEKANYCP 1370 VQNDLAYAND 1380 FASEYYLESE 1390 GQPLSAPCPL 1400 LEKEEVIQTS 1410 TKGQLDCVIT 1420 LHKDQDLIKD 1430 PRNLLANQTL 1440 VYSQDLGEMT 1450 KLNSKRVSFK 1460 LPKDQMKVYV 1470 DDIYVIPQPH 1480 FSTDQPPLPK 1490 KGQSSINKEE 1500 VILSKAGNKS 1510 LNIIENSSAP 1520 ICENKPKILN 1530 SEEWFAAACK 1540 KELKENIQTT 1550 NYNTALDFHS 1560 NSDVTKQVIQ 1570 THVNAGEAPD 1580 PVITSNVPCF 1590 HSIKPNLNNL 1600 NGKTGEFLAF 1610 QTVHLPPLPE 1620 QLLELGNKAH 1630 NDMHIVQATE 1640 IHNINIISSN 1650 AKDSRDEENK 1660 KSHNGAETTS 1670 LPPKTVFKDK 1680 VRRCSLGIFL 1690 PRLPNKRNCS 1700 VTGIDDLEQI 1710 PADTTDINHL 1720 ETQPVSSKDS 1730 GIGSVAGKLN 1740 LSPSQYINEE 1750 NLPVYPDEIN 1760 SSDSINIETE 1770 EKALIETYQK 1780 EISPYENKMG 1790 KTCNSQKRTW 1800 VQEEEDIHKE 1810 KKIRKNEIKF 1820 SDTTQDREIF 1830 DHHTEEDIDK 1840 SANSVLIKNL 1850 SRTPSSCSSS 1860 LDSIKADGTS 1870 LDFSTYRSSQ 1880 MESQFLRDTI 1890 CEESLREKLQ 1900 DGRITIREFF 1910 ILLQVHILIQ 1920 KPRQSNLPGN 1930 FTVNTPPTPE 1940 DLMLSQYVYR 1950 PKIQIYREDC 1960 EARRQKIEEL 1970 KLSASNQDKL 1980 LVDINKNLWE 1990 KMRHCSDKEL 2000 KAFGIYLNKI 2010 KSCFTKMTKV 2020 FTHQGKVALY 2030 GKLVQSAQNE 2040 REKLQIKIDE 2050 MDKILKKIDN 2060 CLTEMETETK 2070 NLEDEEKNNP 2080 VEEWDSEMRA 2090 AEKELEQLKT 2100 EEEELQRNLL 2110 ELEVQKEQTL 2120 AQIDFMQKQR 2130 NRTEELLDQL 2140 SLSEWDVVEW 2150 SDDQAVFTFV 2160 YDTIQLTITF 2170 EESVVGFPFL 2180 DKRYRKIVDV 2190 NFQSLLDEDQ 2200 APPSSLLVHK 2210 LIFQYVEEKE 2220 SWKKTCTTQH 2230 QLPKMLEEFS 2240 LVVHHCRLLG 2250 EEIEYLKRWG 2260 PNYNLMNIDI 2270 NNNELRLLFS 2280 SSAAFAKFEI 2290 TLFLSAYYPS 2300 VPLPSTIQNH 2310 VGNTSQDDIA 2320 TILSKVPLEN 2330 NYLKNVVKQI 2340 YQDLFQDCHF YH

Gene Ontology

Classification GO ID Description
Cellular Component GO:0001669 acrosomal vesicle
Cellular Component GO:0005829 cytosol
Cellular Component GO:0000776 kinetochore
Cellular Component GO:0180019 Knl1/Spc105 complex
Cellular Component GO:0016604 nuclear body
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0000940 outer kinetochore
Molecular Function GO:0008017 microtubule binding
Biological Process GO:0001675 acrosome assembly
Biological Process GO:0008608 attachment of spindle microtubules to kinetochore
Biological Process GO:0051301 cell division
Biological Process GO:0031619 homologous chromosome orientation in meiotic metaphase I
Biological Process GO:0000070 mitotic sister chromatid segregation
Biological Process GO:1905326 positive regulation of meiosis I spindle assembly checkpoint
Biological Process GO:0034501 protein localization to kinetochore
Biological Process GO:0090266 regulation of mitotic cell cycle spindle assembly checkpoint

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[3] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.