Overview
| Uniprot ID | Q8NHH9 |
|---|
| Protein Name | Atlastin-2 |
|---|
| Gene Name | ATL2 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 406 |
EQVCGGDKPYIAPSD |
| 461 |
ETYANFIKHNDGKNI |
Function
Atlastin-2 (ATL2) is a membrane-anchored GTPase that mediates the GTP-dependent fusion of endoplasmic reticulum (ER) membranes, maintaining the continuous ER network. It facilitates the formation of three-way junctions where ER tubules intersect (PubMed:18270207, PubMed:19665976, PubMed:22065636, PubMed:27619977, PubMed:34817557). Two atlastin-2 on neighboring ER tubules bind GTP and form loose homodimers through the GB1/RHD3-type G domains and 3HB regions. Upon GTP hydrolysis, the 3HB regions tighten, pulling the membranes together to drive their fusion. After fusion, the homodimer disassembles upon release of inorganic phosphate (Pi). Subsequently, GDP dissociates, resetting the monomers to a conformation ready for a new fusion cycle (By similarity)
Protein Sequence
10
MAEGDEAARG
20
QQPHQGLWRR
30
RRTSDPSAAV
40
NHVSSTTSLG
50
ENYEDDDLVN
60
SDEVMKKPCP
70
VQIVLAHEDD
80
HNFELDEEAL
90
EQILLQEHIR
100
DLNIVVVSVA
110
GAFRKGKSFL
120
LDFMLRYMYN
130
KDSQSWIGGN
140
NEPLTGFTWR
150
GGCERETTGI
160
QVWNEVFVID
170
RPNGTKVAVL
180
LMDTQGAFDS
190
QSTIKDCATV
200
FALSTMTSSV
210
QVYNLSQNIQ
220
EDDLQHLQLF
230
TEYGRLAMEE
240
IYQKPFQTLM
250
FLIRDWSYPY
260
EHSYGLEGGK
270
QFLEKRLQVK
280
QNQHEELQNV
290
RKHIHNCFSN
300
LGCFLLPHPG
310
LKVATNPSFD
320
GRLKDIDEDF
330
KRELRNLVPL
340
LLAPENLVEK
350
EISGSKVTCR
360
DLVEYFKAYI
370
KIYQGEELPH
380
PKSMLQATAE
390
ANNLAAVAGA
400
RDTYCKSMEQ
410
VCGGDKPYIA
420
PSDLERKHLD
430
LKEVAIKQFR
440
SVKKMGGDEF
450
CRRYQDQLEA
460
EIEETYANFI
470
KHNDGKNIFY
480
AARTPATLFA
490
VMFAMYIISG
500
LTGFIGLNSI
510
AVLCNLVMGL
520
ALIFLCTWAY
530
VKYSGEFREI
540
GTVIDQIAET
550
LWEQVLKPLG
560
DNLMEENIRQ
570
SVTNSIKAGL
580
TDQVSHHARL
KTD
Gene Ontology
| Classification |
GO ID |
Description |
| Molecular Function |
GO:0005525 |
GTP binding |
| Cellular Component |
GO:0005783 |
endoplasmic reticulum |
| Cellular Component |
GO:0005789 |
endoplasmic reticulum membrane |
| Cellular Component |
GO:0098826 |
endoplasmic reticulum tubular network membrane |
| Cellular Component |
GO:0016020 |
membrane |
| Molecular Function |
GO:0003924 |
GTPase activity |
| Molecular Function |
GO:0140523 |
GTPase-dependent fusogenic activity |
| Molecular Function |
GO:0046872 |
metal ion binding |
| Biological Process |
GO:0016320 |
endoplasmic reticulum membrane fusion |
| Biological Process |
GO:0007029 |
endoplasmic reticulum organization |
| Biological Process |
GO:1990809 |
endoplasmic reticulum tubular network membrane organization |
| Biological Process |
GO:0051260 |
protein homooligomerization |
Reference
[1] Cheng Z, Huang H, Li M, Chen Y. Proteomic analysis identifies PFKP lactylation in SW480 colon cancer cells.. iScience 27(1):108645. 2024 Jan 19. PMID: 38155775.