Search Results

Overview

Uniprot IDQ8NI27
Protein NameTHO complex subunit 2
Gene NameTHOC2
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
1239 ERSQCGVKAVNKASS
1243 CGVKAVNKASSTTPK
1284 KEKEKKEKTPATTPE
1343 KAKDEKFKTTVPNAE
1352 TVPNAESKSTQERER
1392 TPVSGSLKSPVPRSD
1411 EREQKRRKIDTHPSP
1449 HTPPPLSKSKEREMD
1451 PPPLSKSKEREMDKK
147 QSQQFNQKSVKIKTK
1552 FKSEKMDKISSGGKK
158 IKTKLFYKQQKFNLL
312 EAKQIVRKLTMVVLS

Function

Component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA (PubMed:15833825, PubMed:15998806, PubMed:17190602). Required for efficient export of polyadenylated RNA and spliced mRNA (PubMed:23222130). The THOC1-THOC2-THOC3 core complex alone is sufficient to bind export factor NXF1-NXT1 and promote ATPase activity of DDX39B; in the complex THOC2 is the only component that directly interacts with DDX39B (PubMed:33191911). TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NXF1 pathway (PubMed:15833825, PubMed:15998806, PubMed:17190602). Required for NXF1 localization to the nuclear rim (PubMed:22893130). THOC2 (and probably the THO complex) is involved in releasing mRNA from nuclear speckle domains

Protein Sequence

10 MAAAAVVVPA 20 EWIKNWEKSG 30 RGEFLHLCRI 40 LSENKSHDSS 50 TYRDFQQALY 60 ELSYHVIKGN 70 LKHEQASNVL 80 SDISEFREDM 90 PSILADVFCI 100 LDIETNCLEE 110 KSKRDYFTQL 120 VLACLYLVSD 130 TVLKERLDPE 140 TLESLGLIKQ 150 SQQFNQKSVK 160 IKTKLFYKQQ 170 KFNLLREENE 180 GYAKLIAELG 190 QDLSGSITSD 200 LILENIKSLI 210 GCFNLDPNRV 220 LDVILEVFEC 230 RPEHDDFFIS 240 LLESYMSMCE 250 PQTLCHILGF 260 KFKFYQEPNG 270 ETPSSLYRVA 280 AVLLQFNLID 290 LDDLYVHLLP 300 ADNCIMDEHK 310 REIAEAKQIV 320 RKLTMVVLSS 330 EKMDEREKEK 340 EKEEEKVEKP 350 PDNQKLGLLE 360 ALLKIGDWQH 370 AQNIMDQMPP 380 YYAASHKLIA 390 LAICKLIHIT 400 IEPLYRRVGV 410 PKGAKGSPVN 420 ALQNKRAPKQ 430 AESFEDLRRD 440 VFNMFCYLGP 450 HLSHDPILFA 460 KVVRIGKSFM 470 KEFQSDGSKQ 480 EDKEKTEVIL 490 SCLLSITDQV 500 LLPSLSLMDC 510 NACMSEELWG 520 MFKTFPYQHR 530 YRLYGQWKNE 540 TYNSHPLLVK 550 VKAQTIDRAK 560 YIMKRLTKEN 570 VKPSGRQIGK 580 LSHSNPTILF 590 DYILSQIQKY 600 DNLITPVVDS 610 LKYLTSLNYD 620 VLAYCIIEAL 630 ANPEKERMKH 640 DDTTISSWLQ 650 SLASFCGAVF 660 RKYPIDLAGL 670 LQYVANQLKA 680 GKSFDLLILK 690 EVVQKMAGIE 700 ITEEMTMEQL 710 EAMTGGEQLK 720 AEGGYFGQIR 730 NTKKSSQRLK 740 DALLDHDLAL 750 PLCLLMAQQR 760 NGVIFQEGGE 770 KHLKLVGKLY 780 DQCHDTLVQF 790 GGFLASNLST 800 EDYIKRVPSI 810 DVLCNEFHTP 820 HDAAFFLSRP 830 MYAHHISSKY 840 DELKKSEKGS 850 KQQHKVHKYI 860 TSCEMVMAPV 870 HEAVVSLHVS 880 KVWDDISPQF 890 YATFWSLTMY 900 DLAVPHTSYE 910 REVNKLKVQM 920 KAIDDNQEMP 930 PNKKKKEKER 940 CTALQDKLLE 950 EEKKQMEHVQ 960 RVLQRLKLEK 970 DNWLLAKSTK 980 NETITKFLQL 990 CIFPRCIFSA 1000 IDAVYCARFV 1010 ELVHQQKTPN 1020 FSTLLCYDRV 1030 FSDIIYTVAS 1040 CTENEASRYG 1050 RFLCCMLETV 1060 TRWHSDRATY 1070 EKECGNYPGF 1080 LTILRATGFD 1090 GGNKADQLDY 1100 ENFRHVVHKW 1110 HYKLTKASVH 1120 CLETGEYTHI 1130 RNILIVLTKI 1140 LPWYPKVLNL 1150 GQALERRVHK 1160 ICQEEKEKRP 1170 DLYALAMGYS 1180 GQLKSRKSYM 1190 IPENEFHHKD 1200 PPPRNAVASV 1210 QNGPGGGPSS 1220 SSIGSASKSD 1230 ESSTEETDKS 1240 RERSQCGVKA 1250 VNKASSTTPK 1260 GNSSNGNSGS 1270 NSNKAVKEND 1280 KEKGKEKEKE 1290 KKEKTPATTP 1300 EARVLGKDGK 1310 EKPKEERPNK 1320 DEKARETKER 1330 TPKSDKEKEK 1340 FKKEEKAKDE 1350 KFKTTVPNAE 1360 SKSTQERERE 1370 KEPSRERDIA 1380 KEMKSKENVK 1390 GGEKTPVSGS 1400 LKSPVPRSDI 1410 PEPEREQKRR 1420 KIDTHPSPSH 1430 SSTVKDSLIE 1440 LKESSAKLYI 1450 NHTPPPLSKS 1460 KEREMDKKDL 1470 DKSRERSRER 1480 EKKDEKDRKE 1490 RKRDHSNNDR 1500 EVPPDLTKRR 1510 KEENGTMGVS 1520 KHKSESPCES 1530 PYPNEKDKEK 1540 NKSKSSGKEK 1550 GSDSFKSEKM 1560 DKISSGGKKE 1570 SRHDKEKIEK 1580 KEKRDSSGGK 1590 EEKKHHKSSD KHR

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0016607 nuclear speck
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0000347 THO complex
Cellular Component GO:0000445 THO complex part of transcription export complex
Cellular Component GO:0000346 transcription export complex
Molecular Function GO:0003729 mRNA binding
Biological Process GO:0048699 generation of neurons
Biological Process GO:0006406 mRNA export from nucleus
Biological Process GO:0006397 mRNA processing
Biological Process GO:0010977 negative regulation of neuron projection development
Biological Process GO:0048666 neuron development
Biological Process GO:0016973 poly(A)+ mRNA export from nucleus
Biological Process GO:0008380 RNA splicing

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[4] Bao Q, Wan N, He Z, Cao J, Yuan W et al.. Subcellular Proteomic Mapping of Lysine Lactylation.. J Am Soc Mass Spectrom 35(12):3221-3232. 2024 Dec 4. PMID: 39569522.

[5] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[6] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.