Search Results

Overview

Uniprot IDQ8QZT1
Protein NameAcetyl-CoA acetyltransferase, mitochondrial
Gene NameAcat1
OrganismMus musculus

Kla Sites from experimental identification

Position Flanking peptide
171 ATPYGGVKLEDLIVK
178 KLEDLIVKDGLTDVY
187 GLTDVYNKIHMGNCA
199 NCAENTAKKMNISRQ
220 LSSYTRSKEAWDAGK
240 TPITISVKGKPDVVV
242 ITISVKGKPDVVVKE
248 GKPDVVVKEDEEYKR
260 YKRVDFSKVPKLKTV
265 FSKVPKLKTVFQKEN
304 AAQRLNVKPLARIAA
335 YAVPKVLKYAGLKKE
75 AIQGAIEKAGIPKEE

Function

This is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA. Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms. The activity of the enzyme is reversible and it can also catalyze the condensation of two acetyl-CoA molecules into acetoacetyl-CoA. Thereby, it plays a major role in ketone body metabolism

Protein Sequence

10 MAALVALHGV 20 VRRPLLRGLL 30 QEVRCLERSY 40 ASKPTLNEVV 50 IVSAIRTPIG 60 SFLGSLASQP 70 ATKLGTAAIQ 80 GAIEKAGIPK 90 EEVKEVYMGN 100 VIQGGEGQAP 110 TRQATLGAGL 120 PISTPCTTVN 130 KVCASGMKAI 140 MMASQSLMCG 150 HQDVMVAGGM 160 ESMSNVPYVM 170 SRGATPYGGV 180 KLEDLIVKDG 190 LTDVYNKIHM 200 GNCAENTAKK 210 MNISRQEQDT 220 YALSSYTRSK 230 EAWDAGKFAS 240 EITPITISVK 250 GKPDVVVKED 260 EEYKRVDFSK 270 VPKLKTVFQK 280 ENGTITAANA 290 STLNDGAAAL 300 VLMTAEAAQR 310 LNVKPLARIA 320 AFADAAVDPI 330 DFPLAPAYAV 340 PKVLKYAGLK 350 KEDIAMWEVN 360 EAFSVVVLAN 370 IKMLEIDPQK 380 VNIHGGAVSL 390 GHPIGMSGAR 400 IVVHMAHALK 410 PGEFGLASIC 420 NGGGGASALL IEKL

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005783 endoplasmic reticulum
Cellular Component GO:0005743 mitochondrial inner membrane
Cellular Component GO:0005759 mitochondrial matrix
Cellular Component GO:0005739 mitochondrion
Molecular Function GO:0003985 acetyl-CoA C-acetyltransferase activity
Molecular Function GO:0016453 C-acetyltransferase activity
Molecular Function GO:0034736 cholesterol O-acyltransferase activity
Molecular Function GO:0120225 coenzyme A binding
Molecular Function GO:0019899 enzyme binding
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0030955 potassium ion binding
Biological Process GO:0006085 acetyl-CoA biosynthetic process
Biological Process GO:0046356 acetyl-CoA catabolic process
Biological Process GO:0060612 adipose tissue development
Biological Process GO:0015937 coenzyme A biosynthetic process
Biological Process GO:0015936 coenzyme A metabolic process
Biological Process GO:0006635 fatty acid beta-oxidation
Biological Process GO:0046952 ketone body catabolic process
Biological Process GO:0006550 L-isoleucine catabolic process
Biological Process GO:0001889 liver development
Biological Process GO:0072229 metanephric proximal convoluted tubule development
Biological Process GO:1902860 propionyl-CoA biosynthetic process
Biological Process GO:0009725 response to hormone
Biological Process GO:0042594 response to starvation

Reference

[1] Chang J, Wu W, Qian P, Lu Z, He X et al.. Multi-omics study on the effect of moderate-intensity exercise on protein lactylation in mouse muscle tissue.. Front Cell Dev Biol 12:1472338. 2024. PMID: 39935788.

[2] Zhuo W, Zhang M, Tan J, Gao Y, Wang Y et al.. Lysine lactylation analysis of proteins in the heart of the Kawasaki disease mouse model.. Front Cell Dev Biol 13:1550220. 2025. PMID: 40114965.

[3] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.