Overview
| Uniprot ID | Q8R123 |
|---|
| Protein Name | Bifunctional FAD diphosphatase/FAD synthase |
|---|
| Gene Name | Flad1 |
|---|
| Organism | Mus musculus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 283 |
ESGSCLGKKVAGALQ |
Function
This enzyme has two activities: FAD diphosphatase activity and FAD synthase activity. FAD diphosphatase acts on FAD and NADH to produce FMN and NMNH(2-), respectively. FAD synthase catalyzes the adenylation of flavin mononucleotide (FMN) to form flavin adenine dinucleotide (FAD) coenzyme. In addition to its catalytic activities, the protein also facilitates the delivery of FAD to client apo-flavoproteins. The balance between FAD synthesis and hydrolysis may be regulated by redox-sensing cysteine residues. At a much lower rate, FAD synthase catalyzes the reverse pyrophosphorolytic reaction
Protein Sequence
10
MASRASELPP
20
GSGRSVTAGI
30
IIVGDEILKG
40
HTQDTNTYFL
50
CRTLRSLGVQ
60
VCRVSVVPDE
70
VATIASEVNS
80
FSRRFTHVLT
90
AGGIGPTHDD
100
VTFEAVAQAF
110
GEELKPHPEL
120
QAAIKTLGGE
130
GWEKLSMVPS
140
SARLHYGTDP
150
RTGHPFRFPL
160
VSVRNVYLFP
170
GIPELLRRVL
180
EGLKGLFQNT
190
AVQFHLKELY
200
VAASEGSIAP
210
ILSEAQAHFG
220
RRLSLGSYPD
230
WSSNYFQVKL
240
ILDSEEKEPL
250
EECLAYLTAR
260
LPQGSLVPYQ
270
PDAVEKAGEA
280
VYKLAESGSC
290
LGKKVAGALQ
300
TIETALAQYH
310
LSQLCVGFNG
320
GKDCTALLHL
330
FHAAVQRKFP
340
DVPKPLQILY
350
IRSISPFPEL
360
EQFLQDTIKR
370
YNLQVLEAEG
380
NMKQALGELQ
390
EKHPQLEAVL
400
MGTRRTDPYS
410
CSLSHFSPTD
420
PGWPSFMRIN
430
PLLDWTYRNI
440
WEFLRQLFVP
450
YCILYDRGYT
460
SLGSRENTTQ
470
NPALKCLSPG
480
GHPVYRPAYL
490
LENEDEERNS
RM
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0005759 |
mitochondrial matrix |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0005886 |
plasma membrane |
| Cellular Component |
GO:0097229 |
sperm end piece |
| Cellular Component |
GO:0097225 |
sperm midpiece |
| Cellular Component |
GO:0097228 |
sperm principal piece |
| Molecular Function |
GO:0005524 |
ATP binding |
| Molecular Function |
GO:0047884 |
FAD diphosphatase activity |
| Molecular Function |
GO:0003919 |
FMN adenylyltransferase activity |
| Molecular Function |
GO:0042802 |
identical protein binding |
| Molecular Function |
GO:0046872 |
metal ion binding |
| Molecular Function |
GO:0035529 |
NADH pyrophosphatase activity |
| Biological Process |
GO:0006747 |
FAD biosynthetic process |
| Biological Process |
GO:0006771 |
riboflavin metabolic process |
Reference
[1] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.