Overview
| Uniprot ID | Q8TAF3 |
|---|
| Protein Name | WD repeat-containing protein 48 |
|---|
| Gene Name | WDR48 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 578 |
PHASSGAKTLKKDRL |
Function
Regulator of deubiquitinating complexes, which acts as a strong activator of USP1, USP12 and USP46 (PubMed:18082604, PubMed:19075014, PubMed:26388029, PubMed:31253762). Enhances the USP1-mediated deubiquitination of FANCD2; USP1 being almost inactive by itself (PubMed:18082604, PubMed:31253762). Activates deubiquitination by increasing the catalytic turnover without increasing the affinity of deubiquitinating enzymes for the substrate (PubMed:19075014, PubMed:27373336). Also activates deubiquitinating activity of complexes containing USP12 (PubMed:19075014, PubMed:27373336, PubMed:27650958). In complex with USP12, acts as a potential tumor suppressor by positively regulating PHLPP1 stability (PubMed:24145035). Docks at the distal end of the USP12 fingers domain and induces a cascade of structural changes leading to the activation of the enzyme (PubMed:27373336, PubMed:27650958). Together with RAD51AP1, promotes DNA repair by stimulating RAD51-mediated homologous recombination (PubMed:27239033, PubMed:27463890, PubMed:32350107). Binds single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) (PubMed:27239033, PubMed:31253762, PubMed:32350107). DNA-binding is required both for USP1-mediated deubiquitination of FANCD2 and stimulation of RAD51-mediated homologous recombination: both WDR48/UAF1 and RAD51AP1 have coordinated role in DNA-binding during these processes (PubMed:31253762, PubMed:32350107). Together with ATAD5 and by regulating USP1 activity, has a role in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA (PubMed:20147293). Together with ATAD5, has a role in recruiting RAD51 to stalled forks during replication stress (PubMed:31844045)
Protein Sequence
10
MAAHHRQNTA
20
GRRKVQVSYV
30
IRDEVEKYNR
40
NGVNALQLDP
50
ALNRLFTAGR
60
DSIIRIWSVN
70
QHKQDPYIAS
80
MEHHTDWVND
90
IVLCCNGKTL
100
ISASSDTTVK
110
VWNAHKGFCM
120
STLRTHKDYV
130
KALAYAKDKE
140
LVASAGLDRQ
150
IFLWDVNTLT
160
ALTASNNTVT
170
TSSLSGNKDS
180
IYSLAMNQLG
190
TIIVSGSTEK
200
VLRVWDPRTC
210
AKLMKLKGHT
220
DNVKALLLNR
230
DGTQCLSGSS
240
DGTIRLWSLG
250
QQRCIATYRV
260
HDEGVWALQV
270
NDAFTHVYSG
280
GRDRKIYCTD
290
LRNPDIRVLI
300
CEEKAPVLKM
310
ELDRSADPPP
320
AIWVATTKST
330
VNKWTLKGIH
340
NFRASGDYDN
350
DCTNPITPLC
360
TQPDQVIKGG
370
ASIIQCHILN
380
DKRHILTKDT
390
NNNVAYWDVL
400
KACKVEDLGK
410
VDFEDEIKKR
420
FKMVYVPNWF
430
SVDLKTGMLT
440
ITLDESDCFA
450
AWVSAKDAGF
460
SSPDGSDPKL
470
NLGGLLLQAL
480
LEYWPRTHVN
490
PMDEEENEVN
500
HVNGEQENRV
510
QKGNGYFQVP
520
PHTPVIFGEA
530
GGRTLFRLLC
540
RDSGGETESM
550
LLNETVPQWV
560
IDITVDKNMP
570
KFNKIPFYLQ
580
PHASSGAKTL
590
KKDRLSASDM
600
LQVRKVMEHV
610
YEKIINLDNE
620
SQTTSSSNNE
630
KPGEQEKEED
640
IAVLAEEKIE
650
LLCQDQVLDP
660
NMDLRTVKHF
670
IWKSGGDLTL
HYRQKST
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0005770 |
late endosome |
| Cellular Component |
GO:0005764 |
lysosome |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0005886 |
plasma membrane |
| Molecular Function |
GO:0035800 |
deubiquitinase activator activity |
| Molecular Function |
GO:0003677 |
DNA binding |
| Molecular Function |
GO:0003690 |
double-stranded DNA binding |
| Molecular Function |
GO:0003697 |
single-stranded DNA binding |
| Molecular Function |
GO:0043130 |
ubiquitin binding |
| Biological Process |
GO:0006974 |
DNA damage response |
| Biological Process |
GO:0000724 |
double-strand break repair via homologous recombination |
| Biological Process |
GO:0090263 |
positive regulation of canonical Wnt signaling pathway |
| Biological Process |
GO:1905168 |
positive regulation of double-strand break repair via homologous recombination |
| Biological Process |
GO:0046427 |
positive regulation of receptor signaling pathway via JAK-STAT |
Reference
[1] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.