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Overview

Uniprot IDQ8TAT6
Protein NameNuclear protein localization protein 4 homolog
Gene NameNPLOC4
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
16 VQSPDGVKRITATKR
179 TGGADKGKFVALENI
62 EITASSNKSLNLLKI

Function

The ternary complex containing UFD1, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope (By similarity). Acts as a negative regulator of type I interferon production via the complex formed with VCP and UFD1, which binds to RIGI and recruits RNF125 to promote ubiquitination and degradation of RIGI (PubMed:26471729)

Protein Sequence

10 MAESIIIRVQ 20 SPDGVKRITA 30 TKRETAATFL 40 KKVAKEFGFQ 50 NNGFSVYINR 60 NKTGEITASS 70 NKSLNLLKIK 80 HGDLLFLFPS 90 SLAGPSSEME 100 TSVPPGFKVF 110 GAPNVVEDEI 120 DQYLSKQDGK 130 IYRSRDPQLC 140 RHGPLGKCVH 150 CVPLEPFDED 160 YLNHLEPPVK 170 HMSFHAYIRK 180 LTGGADKGKF 190 VALENISCKI 200 KSGCEGHLPW 210 PNGICTKCQP 220 SAITLNRQKY 230 RHVDNIMFEN 240 HTVADRFLDF 250 WRKTGNQHFG 260 YLYGRYTEHK 270 DIPLGIRAEV 280 AAIYEPPQIG 290 TQNSLELLED 300 PKAEVVDEIA 310 AKLGLRKVGW 320 IFTDLVSEDT 330 RKGTVRYSRN 340 KDTYFLSSEE 350 CITAGDFQNK 360 HPNMCRLSPD 370 GHFGSKFVTA 380 VATGGPDNQV 390 HFEGYQVSNQ 400 CMALVRDECL 410 LPCKDAPELG 420 YAKESSSEQY 430 VPDVFYKDVD 440 KFGNEITQLA 450 RPLPVEYLII 460 DITTTFPKDP 470 VYTFSISQNP 480 FPIENRDVLG 490 ETQDFHSLAT 500 YLSQNTSSVF 510 LDTISDFHLL 520 LFLVTNEVMP 530 LQDSISLLLE 540 AVRTRNEELA 550 QTWKRSEQWA 560 TIEQLCSTVG 570 GQLPGLHEYG 580 AVGGSTHTAT 590 AAMWACQHCT 600 FMNQPGTGHC EMCSLPRT

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005783 endoplasmic reticulum
Cellular Component GO:0042175 nuclear outer membrane-endoplasmic reticulum membrane network
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0036501 UFD1-NPL4 complex
Cellular Component GO:0034098 VCP-NPL4-UFD1 AAA ATPase complex
Molecular Function GO:0051117 ATPase binding
Molecular Function GO:0036435 K48-linked polyubiquitin modification-dependent protein binding
Molecular Function GO:0070530 K63-linked polyubiquitin modification-dependent protein binding
Molecular Function GO:0044877 protein-containing complex binding
Molecular Function GO:0043130 ubiquitin binding
Molecular Function GO:0031625 ubiquitin protein ligase binding
Molecular Function GO:0008270 zinc ion binding
Biological Process GO:0036503 ERAD pathway
Biological Process GO:0007030 Golgi organization
Biological Process GO:0039536 negative regulation of RIG-I signaling pathway
Biological Process GO:0032480 negative regulation of type I interferon production
Biological Process GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process
Biological Process GO:0072344 rescue of stalled cytosolic ribosome
Biological Process GO:0030970 retrograde protein transport, ER to cytosol
Biological Process GO:1990116 ribosome-associated ubiquitin-dependent protein catabolic process
Biological Process GO:0006511 ubiquitin-dependent protein catabolic process

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.