Overview
| Uniprot ID | Q8TB72 |
|---|
| Protein Name | Pumilio homolog 2 |
|---|
| Gene Name | PUM2 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 672 |
AAPGAEAKYRSASST |
Function
Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE) (, PubMed:21397187). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation (PubMed:22955276). Also mediates deadenylation-independent repression by promoting accessibility of miRNAs (PubMed:18776931, PubMed:22345517). Acts as a post-transcriptional repressor of E2F3 mRNAs by binding to its 3'-UTR and facilitating miRNA regulation (PubMed:22345517). Plays a role in cytoplasmic sensing of viral infection (PubMed:25340845). Represses a program of genes necessary to maintain genomic stability such as key mitotic, DNA repair and DNA replication factors. Its ability to repress those target mRNAs is regulated by the lncRNA NORAD (non-coding RNA activated by DNA damage) which, due to its high abundance and multitude of PUMILIO binding sites, is able to sequester a significant fraction of PUM1 and PUM2 in the cytoplasm (PubMed:26724866). May regulate DCUN1D3 mRNA levels (PubMed:25349211). May support proliferation and self-renewal of stem cells. Binds specifically to miRNA MIR199A precursor, with PUM1, regulates miRNA MIR199A expression at a postranscriptional level (PubMed:28431233)
Protein Sequence
10
MNHDFQALAL
20
ESRGMGELLP
30
TKKFWEPDDS
40
TKDGQKGIFL
50
GDDEWRETAW
60
GASHHSMSQP
70
IMVQRRSGQG
80
FHGNSEVNAI
90
LSPRSESGGL
100
GVSMVEYVLS
110
SSPADKLDSR
120
FRKGNFGTRD
130
AETDGPEKGD
140
QKGKASPFEE
150
DQNRDLKQGD
160
DDDSKINGRG
170
LPNGMDADCK
180
DFNRTPGSRQ
190
ASPTEVVERL
200
GPNTNPSEGL
210
GPLPNPTANK
220
PLVEEFSNPE
230
TQNLDAMEQV
240
GLESLQFDYP
250
GNQVPMDSSG
260
ATVGLFDYNS
270
QQQLFQRTNA
280
LTVQQLTAAQ
290
QQQYALAAAQ
300
QPHIAGVFSA
310
GLAPAAFVPN
320
PYIISAAPPG
330
TDPYTAAGLA
340
AAATLAGPAV
350
VPPQYYGVPW
360
GVYPANLFQQ
370
QAAAAANNTA
380
SQQAASQAQP
390
GQQQVLRAGA
400
GQRPLTPNQG
410
QQGQQAESLA
420
AAAAANPTLA
430
FGQGLATGMP
440
GYQVLAPTAY
450
YDQTGALVVG
460
PGARTGLGAP
470
VRLMAPTPVL
480
ISSAAAQAAA
490
AAAAGGTASS
500
LTGSTNGLFR
510
PIGTQPPQQQ
520
QQQPSTNLQS
530
NSFYGSSSLT
540
NSSQSSSLFS
550
HGPGQPGSTS
560
LGFGSGNSLG
570
AAIGSALSGF
580
GSSVGSSASS
590
SATRRESLST
600
SSDLYKRSSS
610
SLAPIGQPFY
620
NSLGFSSSPS
630
PIGMPLPSQT
640
PGHSLTPPPS
650
LSSHGSSSSL
660
HLGGLTNGSG
670
RYISAAPGAE
680
AKYRSASSTS
690
SLFSSSSQLF
700
PPSRLRYNRS
710
DIMPSGRSRL
720
LEDFRNNRFP
730
NLQLRDLIGH
740
IVEFSQDQHG
750
SRFIQQKLER
760
ATPAERQMVF
770
NEILQAAYQL
780
MTDVFGNYVI
790
QKFFEFGSLD
800
QKLALATRIR
810
GHVLPLALQM
820
YGCRVIQKAL
830
ESISSDQQVI
840
SEMVKELDGH
850
VLKCVKDQNG
860
NHVVQKCIEC
870
VQPQSLQFII
880
DAFKGQVFVL
890
STHPYGCRVI
900
QRILEHCTAE
910
QTLPILEELH
920
QHTEQLVQDQ
930
YGNYVIQHVL
940
EHGRPEDKSK
950
IVSEIRGKVL
960
ALSQHKFASN
970
VVEKCVTHAS
980
RAERALLIDE
990
VCCQNDGPHS
1000
ALYTMMKDQY
1010
ANYVVQKMID
1020
MAEPAQRKII
1030
MHKIRPHITT
1040
LRKYTYGKHI
1050
LAKLEKYYLK
1060
NSPDLGPIGG
PPNGML
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0010494 |
cytoplasmic stress granule |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0098978 |
glutamatergic synapse |
| Cellular Component |
GO:0043025 |
neuronal cell body |
| Cellular Component |
GO:0048471 |
perinuclear region of cytoplasm |
| Cellular Component |
GO:0098794 |
postsynapse |
| Molecular Function |
GO:0106222 |
lncRNA binding |
| Molecular Function |
GO:0035198 |
miRNA binding |
| Molecular Function |
GO:0003730 |
mRNA 3'-UTR binding |
| Molecular Function |
GO:0062104 |
pumilio-response element binding |
| Molecular Function |
GO:0003723 |
RNA binding |
| Biological Process |
GO:0061158 |
3'-UTR-mediated mRNA destabilization |
| Biological Process |
GO:0060612 |
adipose tissue development |
| Biological Process |
GO:0051276 |
chromosome organization |
| Biological Process |
GO:0001942 |
hair follicle development |
| Biological Process |
GO:0035196 |
miRNA processing |
| Biological Process |
GO:0007005 |
mitochondrion organization |
| Biological Process |
GO:2000637 |
positive regulation of miRNA-mediated gene silencing |
| Biological Process |
GO:1900246 |
positive regulation of RIG-I signaling pathway |
| Biological Process |
GO:0048687 |
positive regulation of sprouting of injured axon |
| Biological Process |
GO:0010608 |
post-transcriptional regulation of gene expression |
| Biological Process |
GO:0051983 |
regulation of chromosome segregation |
| Biological Process |
GO:1904580 |
regulation of intracellular mRNA localization |
| Biological Process |
GO:0060964 |
regulation of miRNA-mediated gene silencing |
| Biological Process |
GO:0043488 |
regulation of mRNA stability |
| Biological Process |
GO:0150052 |
regulation of postsynapse assembly |
| Biological Process |
GO:0006417 |
regulation of translation |
| Biological Process |
GO:0022904 |
respiratory electron transport chain |
| Biological Process |
GO:0001501 |
skeletal system development |
Reference
PMID: N/A