Search Results

Overview

Uniprot IDQ8TCS8
Protein NamePolyribonucleotide nucleotidyltransferase 1, mitochondrial
Gene NamePNPT1
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
315 SRDEAVNKIRLDTEE
407 DSLESGIKSDQVITA
552 FKIAGTNKGITALQA
750 GRMRLSRKVLQSPAT

Function

RNA-binding protein implicated in numerous RNA metabolic processes (PubMed:29967381, PubMed:39019044). Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'-to-5' direction (PubMed:29967381, PubMed:39019044). Mitochondrial intermembrane factor with RNA-processing exoribonulease activity (PubMed:29967381, PubMed:39019044). Component of the mitochondrial degradosome (mtEXO) complex, that degrades 3' overhang double-stranded RNA with a 3'-to-5' directionality in an ATP-dependent manner (PubMed:29967381, PubMed:39019044). Involved in the degradation of non-coding mitochondrial transcripts (MT-ncRNA) and tRNA-like molecules (PubMed:29967381, PubMed:39019044). Required for correct processing and polyadenylation of mitochondrial mRNAs. Plays a role as a cytoplasmic RNA import factor that mediates the translocation of small RNA components, like the 5S RNA, the RNA subunit of ribonuclease P and the mitochondrial RNA-processing (MRP) RNA, into the mitochondrial matrix. Plays a role in mitochondrial morphogenesis and respiration; regulates the expression of the electron transport chain (ETC) components at the mRNA and protein levels. In the cytoplasm, shows a 3'-to-5' exoribonuclease mediating mRNA degradation activity; degrades c-myc mRNA upon treatment with IFNB1/IFN-beta, resulting in a growth arrest in melanoma cells. Regulates the stability of specific mature miRNAs in melanoma cells; specifically and selectively degrades miR-221, preferentially. Also plays a role in RNA cell surveillance by cleaning up oxidized RNAs. Binds to the RNA subunit of ribonuclease P, MRP RNA and miR-221 microRNA

Protein Sequence

10 MAACRYCCSC 20 LRLRPLSDGP 30 FLLPRRDRAL 40 TQLQVRALWS 50 SAGSRAVAVD 60 LGNRKLEISS 70 GKLARFADGS 80 AVVQSGDTAV 90 MVTAVSKTKP 100 SPSQFMPLVV 110 DYRQKAAAAG 120 RIPTNYLRRE 130 IGTSDKEILT 140 SRIIDRSIRP 150 LFPAGYFYDT 160 QVLCNLLAVD 170 GVNEPDVLAI 180 NGASVALSLS 190 DIPWNGPVGA 200 VRIGIIDGEY 210 VVNPTRKEMS 220 SSTLNLVVAG 230 APKSQIVMLE 240 ASAENILQQD 250 FCHAIKVGVK 260 YTQQIIQGIQ 270 QLVKETGVTK 280 RTPQKLFTPS 290 PEIVKYTHKL 300 AMERLYAVFT 310 DYEHDKVSRD 320 EAVNKIRLDT 330 EEQLKEKFPE 340 ADPYEIIESF 350 NVVAKEVFRS 360 IVLNEYKRCD 370 GRDLTSLRNV 380 SCEVDMFKTL 390 HGSALFQRGQ 400 TQVLCTVTFD 410 SLESGIKSDQ 420 VITAINGIKD 430 KNFMLHYEFP 440 PYATNEIGKV 450 TGLNRRELGH 460 GALAEKALYP 470 VIPRDFPFTI 480 RVTSEVLESN 490 GSSSMASACG 500 GSLALMDSGV 510 PISSAVAGVA 520 IGLVTKTDPE 530 KGEIEDYRLL 540 TDILGIEDYN 550 GDMDFKIAGT 560 NKGITALQAD 570 IKLPGIPIKI 580 VMEAIQQASV 590 AKKEILQIMN 600 KTISKPRASR 610 KENGPVVETV 620 QVPLSKRAKF 630 VGPGGYNLKK 640 LQAETGVTIS 650 QVDEETFSVF 660 APTPSAMHEA 670 RDFITEICKD 680 DQEQQLEFGA 690 VYTATITEIR 700 DTGVMVKLYP 710 NMTAVLLHNT 720 QLDQRKIKHP 730 TALGLEVGQE 740 IQVKYFGRDP 750 ADGRMRLSRK 760 VLQSPATTVV 770 RTLNDRSSIV 780 MGEPISQSSS NSQ

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005789 endoplasmic reticulum membrane
Cellular Component GO:0045025 mitochondrial degradosome
Cellular Component GO:0005758 mitochondrial intermembrane space
Cellular Component GO:0005759 mitochondrial matrix
Cellular Component GO:0005739 mitochondrion
Cellular Component GO:0005840 ribosome
Molecular Function GO:0000175 3'-5'-RNA exonuclease activity
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0035198 miRNA binding
Molecular Function GO:0034046 poly(G) binding
Molecular Function GO:0008266 poly(U) RNA binding
Molecular Function GO:0004654 polyribonucleotide nucleotidyltransferase activity
Molecular Function GO:0003723 RNA binding
Biological Process GO:0035458 cellular response to interferon-beta
Biological Process GO:0034599 cellular response to oxidative stress
Biological Process GO:0097421 liver regeneration
Biological Process GO:0000958 mitochondrial mRNA catabolic process
Biological Process GO:0097222 mitochondrial mRNA polyadenylation
Biological Process GO:0000965 mitochondrial RNA 3'-end processing
Biological Process GO:0000964 mitochondrial RNA 5'-end processing
Biological Process GO:0000957 mitochondrial RNA catabolic process
Biological Process GO:0007005 mitochondrion organization
Biological Process GO:0006402 mRNA catabolic process
Biological Process GO:0006397 mRNA processing
Biological Process GO:0071042 nuclear polyadenylation-dependent mRNA catabolic process
Biological Process GO:2000627 positive regulation of miRNA catabolic process
Biological Process GO:0000962 positive regulation of mitochondrial RNA catabolic process
Biological Process GO:0061014 positive regulation of mRNA catabolic process
Biological Process GO:0051260 protein homooligomerization
Biological Process GO:0070207 protein homotrimerization
Biological Process GO:0043457 regulation of cellular respiration
Biological Process GO:2000772 regulation of cellular senescence
Biological Process GO:0051591 response to cAMP
Biological Process GO:0060416 response to growth hormone
Biological Process GO:0006401 RNA catabolic process
Biological Process GO:0035927 RNA import into mitochondrion
Biological Process GO:0035928 rRNA import into mitochondrion

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[3] Yan M, Tu H, Tang S, Gai Z, Shi Q et al.. Lactylated Proteomic Analysis Reveals Functional Implications of Lysine Lactylation In Asthenozoospermia.. Mol Cell Proteomics 24(12):101439. 2025 Dec. PMID: 41192556.