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Overview

Uniprot IDQ8TDD1
Protein NameATP-dependent RNA helicase DDX54
Gene NameDDX54
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
58 EDDARARKLGPGRPL
731 TRGRQQLKWDRKKKR
764 RYISSSYKRDLYQKW
770 YKRDLYQKWKQKQKI
838 KQRRRAQKLHFLQRG

Function

Nucleolar RNA helicase that is involved in diverse cellular processes including ribosome biogenesis, DNA damage response, RNA splicing, transcriptional regulation, innate immunity, and nervous system development. Plays an essential role in the formation of the ribosome active site by remodeling rRNA structure and initiating peptidyl transferase center formation (PubMed:38632236). Represses the transcriptional activity of several nuclear receptors. Regulates transcriptome dynamics during DNA damage response. Mechanistically, acts via increased interaction with a well-defined class of pre-mRNAs that contain introns with weak acceptor splice sites, as well as by protein-protein contacts within components of U2 snRNP and spliceosomal B complex. These activities lead to reduced intron retention and enhanced processing rates of its target transcripts (PubMed:28596291). Acts as an inhibitor of type I interferon antiviral response by facilitating ALKBH5-mediated demethylation of specific transcripts (PubMed:40793791). Contributes to central nervous system myelination, likely through regulation of oligodendrocyte differentiation and myelin sheath formation (By similarity)

Protein Sequence

10 MAADKGPAAG 20 PRSRAAMAQW 30 RKKKGLRKRR 40 GAASQARGSD 50 SEDGEFEIQA 60 EDDARARKLG 70 PGRPLPTFPT 80 SECTSDVEPD 90 TREMVRAQNK 100 KKKKSGGFQS 110 MGLSYPVFKG 120 IMKKGYKVPT 130 PIQRKTIPVI 140 LDGKDVVAMA 150 RTGSGKTACF 160 LLPMFERLKT 170 HSAQTGARAL 180 ILSPTRELAL 190 QTLKFTKELG 200 KFTGLKTALI 210 LGGDRMEDQF 220 AALHENPDII 230 IATPGRLVHV 240 AVEMSLKLQS 250 VEYVVFDEAD 260 RLFEMGFAEQ 270 LQEIIARLPG 280 GHQTVLFSAT 290 LPKLLVEFAR 300 AGLTEPVLIR 310 LDVDTKLNEQ 320 LKTSFFLVRE 330 DTKAAVLLHL 340 LHNVVRPQDQ 350 TVVFVATKHH 360 AEYLTELLTT 370 QRVSCAHIYS 380 ALDPTARKIN 390 LAKFTLGKCS 400 TLIVTDLAAR 410 GLDIPLLDNV 420 INYSFPAKGK 430 LFLHRVGRVA 440 RAGRSGTAYS 450 LVAPDEIPYL 460 LDLHLFLGRS 470 LTLARPLKEP 480 SGVAGVDGML 490 GRVPQSVVDE 500 EDSGLQSTLE 510 ASLELRGLAR 520 VADNAQQQYV 530 RSRPAPSPES 540 IKRAKEMDLV 550 GLGLHPLFSS 560 RFEEEELQRL 570 RLVDSIKNYR 580 SRATIFEINA 590 SSRDLCSQVM 600 RAKRQKDRKA 610 IARFQQGQQG 620 RQEQQEGPVG 630 PAPSRPALQE 640 KQPEKEEEEE 650 AGESVEDIFS 660 EVVGRKRQRS 670 GPNRGAKRRR 680 EEARQRDQEF 690 YIPYRPKDFD 700 SERGLSISGE 710 GGAFEQQAAG 720 AVLDLMGDEA 730 QNLTRGRQQL 740 KWDRKKKRFV 750 GQSGQEDKKK 760 IKTESGRYIS 770 SSYKRDLYQK 780 WKQKQKIDDR 790 DSDEEGASDR 800 RGPERRGGKR 810 DRGQGASRPH 820 APGTPAGRVR 830 PELKTKQQIL 840 KQRRRAQKLH 850 FLQRGGLKQL 860 SARNRRRVQE 870 LQQGAFGRGA 880 RSKKGKMRKR M

Gene Ontology

Classification GO ID Description
Cellular Component GO:0016020 membrane
Cellular Component GO:0005730 nucleolus
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0016887 ATP hydrolysis activity
Molecular Function GO:0030331 nuclear estrogen receptor binding
Molecular Function GO:0003723 RNA binding
Molecular Function GO:0003724 RNA helicase activity
Molecular Function GO:0005102 signaling receptor binding
Molecular Function GO:0003714 transcription corepressor activity
Biological Process GO:0006351 DNA-templated transcription
Biological Process GO:0030520 estrogen receptor signaling pathway
Biological Process GO:0016070 RNA metabolic process
Biological Process GO:0006396 RNA processing
Biological Process GO:0006364 rRNA processing

Reference

[1] Bao Q, Wan N, He Z, Cao J, Yuan W et al.. Subcellular Proteomic Mapping of Lysine Lactylation.. J Am Soc Mass Spectrom 35(12):3221-3232. 2024 Dec 4. PMID: 39569522.

[2] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.