Overview
| Uniprot ID | Q8TDZ2 |
|---|
| Protein Name | [F-actin]-monooxygenase MICAL1 |
|---|
| Gene Name | MICAL1 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 488 |
DLYDVLAKEPVQRNN |
Function
Monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin to form methionine-sulfoxide, resulting in actin filament disassembly and preventing repolymerization (PubMed:29343822). In the absence of actin, it also functions as a NADPH oxidase producing H(2)O(2) (PubMed:21864500, PubMed:26845023, PubMed:29343822). Acts as a cytoskeletal regulator that connects NEDD9 to intermediate filaments. Also acts as a negative regulator of apoptosis via its interaction with STK38 and STK38L; acts by antagonizing STK38 and STK38L activation by MST1/STK4. Involved in regulation of lamina-specific connectivity in the nervous system such as the development of lamina-restricted hippocampal connections. Through redox regulation of the actin cytoskeleton controls the intracellular distribution of secretory vesicles containing L1/neurofascin/NgCAM family proteins in neurons, thereby regulating their cell surface levels (By similarity). May act as Rab effector protein and play a role in vesicle trafficking. Promotes endosomal tubule extension by associating with RAB8 (RAB8A or RAB8B), RAB10 and GRAF (GRAF1/ARHGAP26 or GRAF2/ARHGAP10) on the endosomal membrane which may connect GRAFs to Rabs, thereby participating in neosynthesized Rab8-Rab10-Rab11-dependent protein export (PubMed:32344433)
Protein Sequence
10
MASPTSTNPA
20
HAHFESFLQA
30
QLCQDVLSSF
40
QELCGALGLE
50
PGGGLPQYHK
60
IKDQLNYWSA
70
KSLWTKLDKR
80
AGQPVYQQGR
90
ACTSTKCLVV
100
GAGPCGLRVA
110
VELALLGARV
120
VLVEKRTKFS
130
RHNVLHLWPF
140
TIHDLRALGA
150
KKFYGRFCTG
160
TLDHISIRQL
170
QLLLLKVALL
180
LGVEIHWGVT
190
FTGLQPPPRK
200
GSGWRAQLQP
210
NPPAQLANYE
220
FDVLISAAGG
230
KFVPEGFKVR
240
EMRGKLAIGI
250
TANFVNGRTV
260
EETQVPEISG
270
VARIYNQSFF
280
QSLLKATGID
290
LENIVYYKDD
300
THYFVMTAKK
310
QCLLRLGVLR
320
QDWPDTNRLL
330
GSANVVPEAL
340
QRFTRAAADF
350
ATHGKLGKLE
360
FAQDAHGQPD
370
VSAFDFTSMM
380
RAESSARVQE
390
KHGARLLLGL
400
VGDCLVEPFW
410
PLGTGVARGF
420
LAAFDAAWMV
430
KRWAEGAESL
440
EVLAERESLY
450
QLLSQTSPEN
460
MHRNVAQYGL
470
DPATRYPNLN
480
LRAVTPNQVR
490
DLYDVLAKEP
500
VQRNNDKTDT
510
GMPATGSAGT
520
QEELLRWCQE
530
QTAGYPGVHV
540
SDLSSSWADG
550
LALCALVYRL
560
QPGLLEPSEL
570
QGLGALEATA
580
WALKVAENEL
590
GITPVVSAQA
600
VVAGSDPLGL
610
IAYLSHFHSA
620
FKSMAHSPGP
630
VSQASPGTSS
640
AVLFLSKLQR
650
TLQRSRAKEN
660
AEDAGGKKLR
670
LEMEAETPST
680
EVPPDPEPGV
690
PLTPPSQHQE
700
AGAGDLCALC
710
GEHLYVLERL
720
CVNGHFFHRS
730
CFRCHTCEAT
740
LWPGGYEQHP
750
GDGHFYCLQH
760
LPQTDHKAEG
770
SDRGPESPEL
780
PTPSENSMPP
790
GLSTPTASQE
800
GAGPVPDPSQ
810
PTRRQIRLSS
820
PERQRLSSLN
830
LTPDPEMEPP
840
PKPPRSCSAL
850
ARHALESSFV
860
GWGLPVQSPQ
870
ALVAMEKEEK
880
ESPFSSEEEE
890
EDVPLDSDVE
900
QALQTFAKTS
910
GTMNNYPTWR
920
RTLLRRAKEE
930
EMKRFCKAQT
940
IQRRLNEIEA
950
ALRELEAEGV
960
KLELALRRQS
970
SSPEQQKKLW
980
VGQLLQLVDK
990
KNSLVAEEAE
1000
LMITVQELNL
1010
EEKQWQLDQE
1020
LRGYMNREEN
1030
LKTAADRQAE
1040
DQVLRKLVDL
1050
VNQRDALIRF
1060
QEERRLSELA
LGTGAQG
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:1990026 |
hippocampal mossy fiber expansion |
| Cellular Component |
GO:0015629 |
actin cytoskeleton |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0010008 |
endosome membrane |
| Cellular Component |
GO:0045171 |
intercellular bridge |
| Cellular Component |
GO:0005882 |
intermediate filament |
| Cellular Component |
GO:0030496 |
midbody |
| Cellular Component |
GO:0005886 |
plasma membrane |
| Molecular Function |
GO:0003779 |
actin binding |
| Molecular Function |
GO:0051015 |
actin filament binding |
| Molecular Function |
GO:0120501 |
F-actin monooxygenase activity |
| Molecular Function |
GO:0071949 |
FAD binding |
| Molecular Function |
GO:0046872 |
metal ion binding |
| Molecular Function |
GO:0016174 |
NAD(P)H oxidase H2O2-forming activity |
| Molecular Function |
GO:0016709 |
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen |
| Molecular Function |
GO:0019901 |
protein kinase binding |
| Molecular Function |
GO:0017124 |
SH3 domain binding |
| Molecular Function |
GO:0031267 |
small GTPase binding |
| Biological Process |
GO:0051017 |
actin filament bundle assembly |
| Biological Process |
GO:0030042 |
actin filament depolymerization |
| Biological Process |
GO:0007010 |
cytoskeleton organization |
| Biological Process |
GO:0043066 |
negative regulation of apoptotic process |
| Biological Process |
GO:1903305 |
regulation of regulated secretory pathway |
| Biological Process |
GO:0007165 |
signal transduction |
| Biological Process |
GO:0019417 |
sulfur oxidation |
Reference
[1] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.