Search Results

Overview

Uniprot IDQ8TEQ6
Protein NameGem-associated protein 5
Gene NameGEMIN5
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
725 SRPPQGKKSIELEKK
732 KSIELEKKRLSQPKA
815 PVSSGFEKSKVTINN
817 SSGFEKSKVTINNKV
823 SKVTINNKVILLKKE
829 NKVILLKKEPPKEKP

Function

The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs (PubMed:16857593, PubMed:18984161, PubMed:20513430, PubMed:33963192). Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP (PubMed:18984161). To assemble core snRNPs, the SMN complex accepts the trapped 5Sm proteins from CLNS1A forming an intermediate (PubMed:18984161). Binding of snRNA inside 5Sm ultimately triggers eviction of the SMN complex, thereby allowing binding of SNRPD3 and SNRPB to complete assembly of the core snRNP. Within the SMN complex, GEMIN5 recognizes and delivers the small nuclear RNAs (snRNAs) to the SMN complex (PubMed:11714716, PubMed:16314521, PubMed:16857593, PubMed:19377484, PubMed:19750007, PubMed:20513430, PubMed:27834343, PubMed:27881600, PubMed:27881601). Binds to the 7-methylguanosine cap of RNA molecules (PubMed:19750007, PubMed:27834343, PubMed:27881600, PubMed:27881601, Ref.27). Binds to the 3'-UTR of SMN1 mRNA and regulates its translation; does not affect mRNA stability (PubMed:25911097). May play a role in the regulation of protein synthesis via its interaction with ribosomes (PubMed:27507887)

Protein Sequence

10 MGQEPRTLPP 20 SPNWYCARCS 30 DAVPGGLFGF 40 AARTSVFLVR 50 VGPGAGESPG 60 TPPFRVIGEL 70 VGHTERVSGF 80 TFSHHPGQYN 90 LCATSSDDGT 100 VKIWDVETKT 110 VVTEHALHQH 120 TISTLHWSPR 130 VKDLIVSGDE 140 KGVVFCYWFN 150 RNDSQHLFIE 160 PRTIFCLTCS 170 PHHEDLVAIG 180 YKDGIVVIID 190 ISKKGEVIHR 200 LRGHDDEIHS 210 IAWCPLPGED 220 CLSINQEETS 230 EEAEITNGNA 240 VAQAPVTKGC 250 YLATGSKDQT 260 IRIWSCSRGR 270 GVMILKLPFL 280 KRRGGGIDPT 290 VKERLWLTLH 300 WPSNQPTQLV 310 SSCFGGELLQ 320 WDLTQSWRRK 330 YTLFSASSEG 340 QNHSRIVFNL 350 CPLQTEDDKQ 360 LLLSTSMDRD 370 VKCWDIATLE 380 CSWTLPSLGG 390 FAYSLAFSSV 400 DIGSLAIGVG 410 DGMIRVWNTL 420 SIKNNYDVKN 430 FWQGVKSKVT 440 ALCWHPTKEG 450 CLAFGTDDGK 460 VGLYDTYSNK 470 PPQISSTYHK 480 KTVYTLAWGP 490 PVPPMSLGGE 500 GDRPSLALYS 510 CGGEGIVLQH 520 NPWKLSGEAF 530 DINKLIRDTN 540 SIKYKLPVHT 550 EISWKADGKI 560 MALGNEDGSI 570 EIFQIPNLKL 580 ICTIQQHHKL 590 VNTISWHHEH 600 GSQPELSYLM 610 ASGSNNAVIY 620 VHNLKTVIES 630 SPESPVTITE 640 PYRTLSGHTA 650 KITSVAWSPH 660 HDGRLVSASY 670 DGTAQVWDAL 680 REEPLCNFRG 690 HRGRLLCVAW 700 SPLDPDCIYS 710 GADDFCVHKW 720 LTSMQDHSRP 730 PQGKKSIELE 740 KKRLSQPKAK 750 PKKKKKPTLR 760 TPVKLESIDG 770 NEEESMKENS 780 GPVENGVSDQ 790 EGEEQAREPE 800 LPCGLAPAVS 810 REPVICTPVS 820 SGFEKSKVTI 830 NNKVILLKKE 840 PPKEKPETLI 850 KKRKARSLLP 860 LSTSLDHRSK 870 EELHQDCLVL 880 ATAKHSRELN 890 EDVSADVEER 900 FHLGLFTDRA 910 TLYRMIDIEG 920 KGHLENGHPE 930 LFHQLMLWKG 940 DLKGVLQTAA 950 ERGELTDNLV 960 AMAPAAGYHV 970 WLWAVEAFAK 980 QLCFQDQYVK 990 AASHLLSIHK 1000 VYEAVELLKS 1010 NHFYREAIAI 1020 AKARLRPEDP 1030 VLKDLYLSWG 1040 TVLERDGHYA 1050 VAAKCYLGAT 1060 CAYDAAKVLA 1070 KKGDAASLRT 1080 AAELAAIVGE 1090 DELSASLALR 1100 CAQELLLANN 1110 WVGAQEALQL 1120 HESLQGQRLV 1130 FCLLELLSRH 1140 LEEKQLSEGK 1150 SSSSYHTWNT 1160 GTEGPFVERV 1170 TAVWKSIFSL 1180 DTPEQYQEAF 1190 QKLQNIKYPS 1200 ATNNTPAKQL 1210 LLHICHDLTL 1220 AVLSQQMASW 1230 DEAVQALLRA 1240 VVRSYDSGSF 1250 TIMQEVYSAF 1260 LPDGCDHLRD 1270 KLGDHQSPAT 1280 PAFKSLEAFF 1290 LYGRLYEFWW 1300 SLSRPCPNSS 1310 VWVRAGHRTL 1320 SVEPSQQLDT 1330 ASTEETDPET 1340 SQPEPNRPSE 1350 LDLRLTEEGE 1360 RMLSTFKELF 1370 SEKHASLQNS 1380 QRTVAEVQET 1390 LAEMIRQHQK 1400 SQLCKSTANG 1410 PDKNEPEVEA 1420 EQPLCSSQSQ 1430 CKEEKNEPLS 1440 LPELTKRLTE 1450 ANQRMAKFPE 1460 SIKAWPFPDV 1470 LECCLVLLLI 1480 RSHFPGCLAQ 1490 EMQQQAQELL 1500 QKYGNTKTYR RHCQTFCM

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0097504 Gemini of Cajal bodies
Cellular Component GO:0016020 membrane
Cellular Component GO:0016604 nuclear body
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0032797 SMN complex
Cellular Component GO:0034718 SMN-Gemin2 complex
Cellular Component GO:0034719 SMN-Sm protein complex
Molecular Function GO:0003730 mRNA 3'-UTR binding
Molecular Function GO:0043022 ribosome binding
Molecular Function GO:0000340 RNA 7-methylguanosine cap binding
Molecular Function GO:0003723 RNA binding
Molecular Function GO:0017069 snRNA binding
Molecular Function GO:0030619 U1 snRNA binding
Molecular Function GO:0030621 U4 snRNA binding
Molecular Function GO:0030622 U4atac snRNA binding
Biological Process GO:0000398 mRNA splicing, via spliceosome
Biological Process GO:0065003 protein-containing complex assembly
Biological Process GO:0006417 regulation of translation
Biological Process GO:0000387 spliceosomal snRNP assembly
Biological Process GO:0006412 translation

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[4] Bao Q, Wan N, He Z, Cao J, Yuan W et al.. Subcellular Proteomic Mapping of Lysine Lactylation.. J Am Soc Mass Spectrom 35(12):3221-3232. 2024 Dec 4. PMID: 39569522.

[5] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.

[6] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[7] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.