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Overview

Uniprot IDQ8VEK3
Protein NameHeterogeneous nuclear ribonucleoprotein U
Gene NameHnrnpu
OrganismMus musculus

Kla Sites from experimental identification

Position Flanking peptide
181 AGKEAAGKSSGPTSL

Function

DNA- and RNA-binding protein involved in several cellular processes such as nuclear chromatin organization, telomere-length regulation, transcription, mRNA alternative splicing and stability, Xist-mediated transcriptional silencing and mitotic cell progression (PubMed:20833368, PubMed:21235343, PubMed:22162999, PubMed:26244333). Plays a role in the regulation of interphase large-scale gene-rich chromatin organization through chromatin-associated RNAs (caRNAs) in a transcription-dependent manner, and thereby maintains genomic stability (By similarity). Required for the localization of the long non-coding Xist RNA on the inactive chromosome X (Xi) and the subsequent initiation and maintenance of X-linked transcriptional gene silencing during X-inactivation (PubMed:20833368, PubMed:26244333). Plays a role as a RNA polymerase II (Pol II) holoenzyme transcription regulator (PubMed:21235343, PubMed:22162999). Promotes transcription initiation by direct association with the core-TFIIH basal transcription factor complex for the assembly of a functional pre-initiation complex with Pol II in a actin-dependent manner. Blocks Pol II transcription elongation activity by inhibiting the C-terminal domain (CTD) phosphorylation of Pol II and dissociates from Pol II pre-initiation complex prior to productive transcription elongation. Positively regulates CBX5-induced transcriptional gene silencing and retention of CBX5 in the nucleus. Negatively regulates glucocorticoid-mediated transcriptional activation (By similarity). Key regulator of transcription initiation and elongation in embryonic stem cells upon leukemia inhibitory factor (LIF) signaling (PubMed:21235343). Involved in the long non-coding RNA H19-mediated Pol II transcriptional repression (By similarity). Participates in the circadian regulation of the core clock component BMAL1 transcription (PubMed:18332112). Plays a role in the regulation of telomere length. Plays a role as a global pre-mRNA alternative splicing modulator by regulating U2 small nuclear ribonucleoprotein (snRNP) biogenesis. Plays a role in mRNA stability. Component of the CRD-mediated complex that promotes MYC mRNA stabilization. Enhances the expression of specific genes, such as tumor necrosis factor TNFA, by regulating mRNA stability, possibly through binding to the 3'-untranslated region (UTR). Plays a role in mitotic cell cycle regulation. Involved in the formation of stable mitotic spindle microtubules (MTs) attachment to kinetochore, spindle organization and chromosome congression. Phosphorylation at Ser-58 by PLK1 is required for chromosome alignement and segregation and progression through mitosis. Also contributes to the targeting of AURKA to mitotic spindle MTs. Binds to double- and single-stranded DNA and RNA, poly(A), poly(C) and poly(G) oligoribonucleotides. Binds to chromatin-associated RNAs (caRNAs). Associates with chromatin to scaffold/matrix attachment region (S/MAR) elements in a chromatin-associated RNAs (caRNAs)-dependent manner (By similarity). Binds (via RNA-binding RGG-box region) to the long non-coding Xist RNA; this binding is direct and bridges the Xist RNA and the inactive chromosome X (Xi) (PubMed:20833368, PubMed:26244333). Binds the long non-coding H19 RNA. Binds to SMN1/2 pre-mRNAs at G/U-rich regions. Binds to small nuclear RNAs (snRNAs). Binds to the 3'-UTR of TNFA mRNA (By similarity). Also negatively regulates embryonic stem cell differentiation upon LIF signaling (PubMed:21235343). Required for embryonic development (PubMed:16022389). Binds to brown fat long non-coding RNA 1 (Blnc1); facilitates the recruitment of Blnc1 by ZBTB7B required to drive brown and beige fat development and thermogenesis (PubMed:28784777)

Protein Sequence

10 MSSSPVNVKK 20 LKVSELKEEL 30 KKRRLSDKGL 40 KADLMDRLQA 50 ALDNEAGGRP 60 AMEPGNGSLD 70 LGGDAAGRSG 80 AGLEQEAAAG 90 AEDDEEEEGI 100 AALDGDQMEL 110 GEENGAAGAA 120 DAGAMEEEEA 130 ASEDENGDDQ 140 GFQEGEDELG 150 DEEEGAGDEN 160 GHGEQQSQPP 170 AAAAQQQPSQ 180 QRGAGKEAAG 190 KSSGPTSLFA 200 VTVAPPGARQ 210 GQQQAGGDGK 220 TEQKGGDKKR 230 GVKRPREDHG 240 RGYFEYIEEN 250 KYSRAKSPQP 260 PVEEEDEHFD 270 DTVVCLDTYN 280 CDLHFKISRD 290 RLSASSLTME 300 SFAFLWAGGR 310 ASYGVSKGKV 320 CFEMKVTEKI 330 PVRHLYTKDI 340 DIHEVRIGWS 350 LTTSGMLLGE 360 EEFSYGYSLK 370 GIKTCNCETE 380 DYGEKFDEND 390 VITCFANFET 400 DEVELSYAKN 410 GQDLGVAFKI 420 SKEVLADRPL 430 FPHVLCHNCA 440 VEFNFGQKEK 450 PYFPIPEDCT 460 FIQNVPLEDR 470 VRGPKGPEEK 480 KDCEVVMMIG 490 LPGAGKTTWV 500 TKHAAENPGK 510 YNILGTNTIM 520 DKMMVAGFKK 530 QMADTGKLNT 540 LLQRAPQCLG 550 KFIEIAARKK 560 RNFILDQTNV 570 SAAAQRRKMC 580 LFAGFQRKAV 590 VVCPKDEDYK 600 QRTQKKAEVE 610 GKDLPEHAVL 620 KMKGNFTLPE 630 VAECFDEITY 640 VELQKEEAQK 650 LLEQYKEESK 660 KALPPEKKQN 670 TGSKKSNKNK 680 SGKNQFNRGG 690 GHRGRGGFNM 700 RGGNFRGGAP 710 GNRGGYNRRG 720 NMPQRGGGGG 730 SGGIGYPYPR 740 GPVFPGRGGY 750 SNRGNYNRGG 760 MPNRGNYNQN 770 FRGRGNNRGY 780 KNQSQGYNQW 790 QQGQFWGQKP 800 WSQHYHQGYY

Gene Ontology

Classification GO ID Description
Cellular Component GO:0071013 catalytic step 2 spliceosome
Cellular Component GO:0009986 cell surface
Cellular Component GO:0005813 centrosome
Cellular Component GO:0070937 CRD-mediated mRNA stability complex
Cellular Component GO:0036464 cytoplasmic ribonucleoprotein granule
Cellular Component GO:0005829 cytosol
Cellular Component GO:0030425 dendrite
Cellular Component GO:0098577 inactive sex chromosome
Cellular Component GO:0000776 kinetochore
Cellular Component GO:0030496 midbody
Cellular Component GO:0072686 mitotic spindle
Cellular Component GO:1990498 mitotic spindle microtubule
Cellular Component GO:1990023 mitotic spindle midzone
Cellular Component GO:0000228 nuclear chromosome
Cellular Component GO:0016363 nuclear matrix
Cellular Component GO:0016607 nuclear speck
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0032991 protein-containing complex
Cellular Component GO:1990904 ribonucleoprotein complex
Cellular Component GO:0090575 RNA polymerase II transcription regulator complex
Cellular Component GO:0000922 spindle pole
Cellular Component GO:0005697 telomerase holoenzyme complex
Molecular Function GO:0003779 actin binding
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0003682 chromatin binding
Molecular Function GO:0031490 chromatin DNA binding
Molecular Function GO:0003677 DNA binding
Molecular Function GO:0003690 double-stranded DNA binding
Molecular Function GO:0003725 double-stranded RNA binding
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0106222 lncRNA binding
Molecular Function GO:0003730 mRNA 3'-UTR binding
Molecular Function GO:0034584 piRNA binding
Molecular Function GO:0008143 poly(A) binding
Molecular Function GO:0017130 poly(C) RNA binding
Molecular Function GO:0034046 poly(G) binding
Molecular Function GO:0036002 pre-mRNA binding
Molecular Function GO:1990841 promoter-specific chromatin binding
Molecular Function GO:0044877 protein-containing complex binding
Molecular Function GO:0043021 ribonucleoprotein complex binding
Molecular Function GO:0003723 RNA binding
Molecular Function GO:0099122 RNA polymerase II C-terminal domain binding
Molecular Function GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding
Molecular Function GO:0000993 RNA polymerase II complex binding
Molecular Function GO:0043565 sequence-specific DNA binding
Molecular Function GO:1990837 sequence-specific double-stranded DNA binding
Molecular Function GO:0003697 single-stranded DNA binding
Molecular Function GO:0003727 single-stranded RNA binding
Molecular Function GO:0017069 snRNA binding
Molecular Function GO:0070034 telomerase RNA binding
Molecular Function GO:0001097 TFIIH-class transcription factor complex binding
Molecular Function GO:0003714 transcription corepressor activity
Biological Process GO:1990845 adaptive thermogenesis
Biological Process GO:0000380 alternative mRNA splicing, via spliceosome
Biological Process GO:0055013 cardiac muscle cell development
Biological Process GO:0051301 cell division
Biological Process GO:0071549 cellular response to dexamethasone stimulus
Biological Process GO:0071385 cellular response to glucocorticoid stimulus
Biological Process GO:1990830 cellular response to leukemia inhibitory factor
Biological Process GO:0032922 circadian regulation of gene expression
Biological Process GO:0070934 CRD-mediated mRNA stabilization
Biological Process GO:0098963 dendritic transport of messenger ribonucleoprotein complex
Biological Process GO:0006351 DNA-templated transcription
Biological Process GO:0009048 dosage compensation by inactivation of X chromosome
Biological Process GO:0030218 erythrocyte differentiation
Biological Process GO:0051457 maintenance of protein location in nucleus
Biological Process GO:0016071 mRNA metabolic process
Biological Process GO:0048255 mRNA stabilization
Biological Process GO:0033673 negative regulation of kinase activity
Biological Process GO:1900152 negative regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
Biological Process GO:2000737 negative regulation of stem cell differentiation
Biological Process GO:0032211 negative regulation of telomere maintenance via telomerase
Biological Process GO:0000122 negative regulation of transcription by RNA polymerase II
Biological Process GO:0034244 negative regulation of transcription elongation by RNA polymerase II
Biological Process GO:1902425 positive regulation of attachment of mitotic spindle microtubules to kinetochore
Biological Process GO:0090336 positive regulation of brown fat cell differentiation
Biological Process GO:2000767 positive regulation of cytoplasmic translation
Biological Process GO:2000373 positive regulation of DNA topoisomerase (ATP-hydrolyzing) activity
Biological Process GO:0010628 positive regulation of gene expression
Biological Process GO:2000648 positive regulation of stem cell proliferation
Biological Process GO:0045944 positive regulation of transcription by RNA polymerase II
Biological Process GO:1902889 protein localization to spindle microtubule
Biological Process GO:0060816 random inactivation of X chromosome
Biological Process GO:0000381 regulation of alternative mRNA splicing, via spliceosome
Biological Process GO:1902275 regulation of chromatin organization
Biological Process GO:0007346 regulation of mitotic cell cycle
Biological Process GO:1901673 regulation of mitotic spindle assembly
Biological Process GO:0031048 regulatory ncRNA-mediated heterochromatin formation
Biological Process GO:1990280 RNA localization to chromatin

Reference

[1] Sung E, Sim H, Cho YC, Lee W, Bae JS et al.. Global Profiling of Lysine Acetylation and Lactylation in Kupffer Cells.. J Proteome Res 22(12):3683-3691. 2023 Dec 1. PMID: 37897433.