Overview
| Uniprot ID | Q8WV22 |
|---|
| Protein Name | Non-structural maintenance of chromosomes element 1 homolog |
|---|
| Gene Name | NSMCE1 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 255 |
ERESGVLKSNKKSLR |
Function
RING-type zinc finger-containing E3 ubiquitin ligase that assembles with melanoma antigen protein (MAGE) to catalyze the direct transfer of ubiquitin from E2 ubiquitin-conjugating enzyme to a specific substrate. Within MAGE-RING ubiquitin ligase complex, MAGE stimulates and specifies ubiquitin ligase activity likely through recruitment and/or stabilization of the E2 ubiquitin-conjugating enzyme at the E3:substrate complex. Involved in maintenance of genome integrity, DNA damage response and DNA repair (PubMed:20864041, PubMed:29225034). NSMCE3/MAGEG1 and NSMCE1 ubiquitin ligase are components of SMC5-SMC6 complex and may positively regulate homologous recombination-mediated DNA repair (PubMed:18086888). MAGEF1-NSMCE1 ubiquitin ligase promotes proteasomal degradation of MMS19, a key component of the cytosolic iron-sulfur protein assembly (CIA) machinery. Down-regulation of MMS19 impairs the activity of several DNA repair and metabolism enzymes such as ERCC2/XPD, FANCJ, RTEL1 and POLD1 that require iron-sulfur clusters as cofactors (PubMed:29225034)
Protein Sequence
10
MQGSTRRMGV
20
MTDVHRRFLQ
30
LLMTHGVLEE
40
WDVKRLQTHC
50
YKVHDRNATV
60
DKLEDFINNI
70
NSVLESLYIE
80
IKRGVTEDDG
90
RPIYALVNLA
100
TTSISKMATD
110
FAENELDLFR
120
KALELIIDSE
130
TGFASSTNIL
140
NLVDQLKGKK
150
MRKKEAEQVL
160
QKFVQNKWLI
170
EKEGEFTLHG
180
RAILEMEQYI
190
RETYPDAVKI
200
CNICHSLLIQ
210
GQSCETCGIR
220
MHLPCVAKYF
230
QSNAEPRCPH
240
CNDYWPHEIP
250
KVFDPEKERE
260
SGVLKSNKKS
LRSRQH
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0000775 |
chromosome, centromeric region |
| Cellular Component |
GO:0000781 |
chromosome, telomeric region |
| Cellular Component |
GO:0097431 |
mitotic spindle pole |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0030915 |
Smc5-Smc6 complex |
| Molecular Function |
GO:0046983 |
protein dimerization activity |
| Molecular Function |
GO:0061630 |
ubiquitin protein ligase activity |
| Molecular Function |
GO:0004842 |
ubiquitin-protein transferase activity |
| Molecular Function |
GO:0008270 |
zinc ion binding |
| Biological Process |
GO:0140588 |
chromatin looping |
| Biological Process |
GO:0006974 |
DNA damage response |
| Biological Process |
GO:0000724 |
double-strand break repair via homologous recombination |
| Biological Process |
GO:0016925 |
protein sumoylation |
| Biological Process |
GO:0032204 |
regulation of telomere maintenance |
Reference
PMID: N/A