Search Results

Overview

Uniprot IDQ8WVC0
Protein NameRNA polymerase-associated protein LEO1
Gene NameLEO1
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
503 TDSATHRKMTLSLAD
591 SISLAAIKNRYKGGI

Function

Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both independently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1; it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Involved in polyadenylation of mRNA precursors. Connects PAF1C to Wnt signaling

Protein Sequence

10 MADMEDLFGS 20 DADSEAERKD 30 SDSGSDSDSD 40 QENAASGSNA 50 SGSESDQDER 60 GDSGQPSNKE 70 LFGDDSEDEG 80 ASHHSGSDNH 90 SERSDNRSEA 100 SERSDHEDND 110 PSDVDQHSGS 120 EAPNDDEDEG 130 HRSDGGSHHS 140 EAEGSEKAHS 150 DDEKWGREDK 160 SDQSDDEKIQ 170 NSDDEERAQG 180 SDEDKLQNSD 190 DDEKMQNTDD 200 EERPQLSDDE 210 RQQLSEEEKA 220 NSDDERPVAS 230 DNDDEKQNSD 240 DEEQPQLSDE 250 EKMQNSDDER 260 PQASDEEHRH 270 SDDEEEQDHK 280 SESARGSDSE 290 DEVLRMKRKN 300 AIASDSEADS 310 DTEVPKDNSG 320 TMDLFGGADD 330 ISSGSDGEDK 340 PPTPGQPVDE 350 NGLPQDQQEE 360 EPIPETRIEV 370 EIPKVNTDLG 380 NDLYFVKLPN 390 FLSVEPRPFD 400 PQYYEDEFED 410 EEMLDEEGRT 420 RLKLKVENTI 430 RWRIRRDEEG 440 NEIKESNARI 450 VKWSDGSMSL 460 HLGNEVFDVY 470 KAPLQGDHNH 480 LFIRQGTGLQ 490 GQAVFKTKLT 500 FRPHSTDSAT 510 HRKMTLSLAD 520 RCSKTQKIRI 530 LPMAGRDPEC 540 QRTEMIKKEE 550 ERLRASIRRE 560 SQQRRMREKQ 570 HQRGLSASYL 580 EPDRYDEEEE 590 GEESISLAAI 600 KNRYKGGIRE 610 ERARIYSSDS 620 DEGSEEDKAQ 630 RLLKAKKLTS 640 DEEGEPSGKR 650 KAEDDDKANK 660 KHKKYVISDE EEEDDD

Gene Ontology

Classification GO ID Description
Cellular Component GO:0016593 Cdc73/Paf1 complex
Cellular Component GO:0001650 fibrillar center
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Molecular Function GO:1990269 RNA polymerase II C-terminal domain phosphoserine binding
Biological Process GO:0001711 endodermal cell fate commitment
Biological Process GO:0031124 mRNA 3'-end processing
Biological Process GO:0045638 negative regulation of myeloid cell differentiation
Biological Process GO:0045944 positive regulation of transcription by RNA polymerase II
Biological Process GO:0032968 positive regulation of transcription elongation by RNA polymerase II
Biological Process GO:0019827 stem cell population maintenance
Biological Process GO:0006368 transcription elongation by RNA polymerase II
Biological Process GO:0016055 Wnt signaling pathway

Reference

[1] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[2] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.