Search Results

Overview

Uniprot IDQ8WWH5
Protein NamePseudouridylate synthase TRUB1
Gene NameTRUB1
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
106 MPSPEWTKRKKQTLK
58 GSEARVSKAALATKL

Function

Pseudouridine synthase that catalyzes pseudouridylation of mRNAs and tRNAs (PubMed:28073919, PubMed:31477916, PubMed:32926445). Mediates pseudouridylation of mRNAs with the consensus sequence 5'-GUUCNANNC-3', harboring a stem-loop structure (PubMed:28073919, PubMed:31477916). Constitutes the major pseudouridine synthase acting on mRNAs (PubMed:28073919). Also catalyzes pseudouridylation of some tRNAs, including synthesis of pseudouridine(55) from uracil-55, in the psi GC loop of a subset of tRNAs (PubMed:32926445, PubMed:33023933). Promotes the processing of pri-let-7 microRNAs (pri-miRNAs) independently of its RNA pseudouridylate synthase activity (PubMed:32926445). Acts by binding to the stem-loop structure on pri-let-7, preventing LIN28-binding (LIN28A and/or LIN28B), thereby enhancing the interaction between pri-let-7 and the microprocessor DGCR8, which mediates miRNA maturation (PubMed:32926445)

Protein Sequence

10 MAASEAAVVS 20 SPSLKTDTSP 30 VLETAGTVAA 40 MAATPSARAA 50 AAVVAAAART 60 GSEARVSKAA 70 LATKLLSLSG 80 VFAVHKPKGP 90 TSAELLNRLK 100 EKLLAEAGMP 110 SPEWTKRKKQ 120 TLKIGHGGTL 130 DSAARGVLVV 140 GIGSGTKMLT 150 SMLSGSKRYT 160 AIGELGKATD 170 TLDSTGRVTE 180 EKPYDKITQE 190 DIEGILQKFT 200 GNIMQVPPLY 210 SALKKDGQRL 220 STLMKRGEVV 230 EAKPARPVTV 240 YSISLQKFQP 250 PFFTLDVECG 260 GGFYIRSLVS 270 DIGKELSSCA 280 NVLELTRTKQ 290 GPFTLEEHAL 300 PEDKWTIDDI 310 AQSLEHCSSL 320 FPAELALKKS 330 KPESNEQVLS 340 CEYITLNEPK REDDVIKTC

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005739 mitochondrion
Cellular Component GO:0005634 nucleus
Molecular Function GO:0070883 pre-miRNA binding
Molecular Function GO:0009982 pseudouridine synthase activity
Molecular Function GO:0160148 tRNA pseudouridine(55) synthase activity
Biological Process GO:0006397 mRNA processing
Biological Process GO:1990481 mRNA pseudouridine synthesis
Biological Process GO:2000633 positive regulation of pre-miRNA processing
Biological Process GO:0006400 tRNA modification
Biological Process GO:0031119 tRNA pseudouridine synthesis

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.