Search Results

Overview

Uniprot IDQ8WWY3
Protein NameU4/U6 small nuclear ribonucleoprotein Prp31
Gene NamePRPF31
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
298 AARLVAAKCTLAARV
417 TQVNEATKARISKTL
422 ATKARISKTLQRTLQ
430 TLQRTLQKQSVVYGG
438 QSVVYGGKSTIRDRS

Function

Involved in pre-mRNA splicing as component of the spliceosome (PubMed:11867543, PubMed:20118938, PubMed:28781166). Required for the assembly of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome (PubMed:11867543)

Protein Sequence

10 MSLADELLAD 20 LEEAAEEEEG 30 GSYGEEEEEP 40 AIEDVQEETQ 50 LDLSGDSVKT 60 IAKLWDSKMF 70 AEIMMKIEEY 80 ISKQAKASEV 90 MGPVEAAPEY 100 RVIVDANNLT 110 VEIENELNII 120 HKFIRDKYSK 130 RFPELESLVP 140 NALDYIRTVK 150 ELGNSLDKCK 160 NNENLQQILT 170 NATIMVVSVT 180 ASTTQGQQLS 190 EEELERLEEA 200 CDMALELNAS 210 KHRIYEYVES 220 RMSFIAPNLS 230 IIIGASTAAK 240 IMGVAGGLTN 250 LSKMPACNIM 260 LLGAQRKTLS 270 GFSSTSVLPH 280 TGYIYHSDIV 290 QSLPPDLRRK 300 AARLVAAKCT 310 LAARVDSFHE 320 STEGKVGYEL 330 KDEIERKFDK 340 WQEPPPVKQV 350 KPLPAPLDGQ 360 RKKRGGRRYR 370 KMKERLGLTE 380 IRKQANRMSF 390 GEIEEDAYQE 400 DLGFSLGHLG 410 KSGSGRVRQT 420 QVNEATKARI 430 SKTLQRTLQK 440 QSVVYGGKST 450 IRDRSSGTAS 460 SVAFTPLQGL 470 EIVNPQAAEK 480 KVAEANQKYF 490 SSMAEFLKVK GEKSGLMST

Gene Ontology

Classification GO ID Description
Cellular Component GO:0015030 Cajal body
Cellular Component GO:0071339 MLL1 complex
Cellular Component GO:0016607 nuclear speck
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0071011 precatalytic spliceosome
Cellular Component GO:0097526 spliceosomal tri-snRNP complex
Cellular Component GO:0071005 U2-type precatalytic spliceosome
Cellular Component GO:0005684 U2-type spliceosomal complex
Cellular Component GO:0005687 U4 snRNP
Cellular Component GO:0046540 U4/U6 x U5 tri-snRNP complex
Cellular Component GO:0005690 U4atac snRNP
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0030674 protein-macromolecule adaptor activity
Molecular Function GO:0043021 ribonucleoprotein complex binding
Molecular Function GO:0003723 RNA binding
Molecular Function GO:0070990 snRNP binding
Molecular Function GO:0030621 U4 snRNA binding
Molecular Function GO:0030622 U4atac snRNA binding
Biological Process GO:0000398 mRNA splicing, via spliceosome
Biological Process GO:0071166 ribonucleoprotein complex localization
Biological Process GO:0000387 spliceosomal snRNP assembly
Biological Process GO:0000244 spliceosomal tri-snRNP complex assembly
Biological Process GO:0000388 spliceosome conformational change to release U4 (or U4atac) and U1 (or U11)

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[4] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.

[5] Chao L, Xu Y, Yang Y, Ao X, Liang J. Identification of lactylation-related biomarkers for diagnosis, prognosis, and treatment responsiveness in triple-negative breast cancer.. World J Surg Oncol 24(1):77. 2026 Jan 22. PMID: 41566505.

[6] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.