Overview
| Uniprot ID | Q8WX92 |
|---|
| Protein Name | Negative elongation factor B |
|---|
| Gene Name | NELFB |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 398 |
RLLALGQGAWDMIDS |
Function
Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II (PubMed:12612062). The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex (PubMed:10199401). May be able to induce chromatin unfolding (PubMed:11739404). Essential for early embryogenesis; plays an important role in maintaining the undifferentiated state of embryonic stem cells (ESCs) by preventing unscheduled expression of developmental genes (By similarity). Plays a key role in establishing the responsiveness of stem cells to developmental cues; facilitates plasticity and cell fate commitment in ESCs by establishing the appropriate expression level of signaling molecules (By similarity). Supports the transcription of genes involved in energy metabolism in cardiomyocytes; facilitates the association of transcription initiation factors with the promoters of the metabolism-related genes (By similarity)
Protein Sequence
10
MAELEGAGER
20
GSGGPRGPAE
30
RASGVSAAAP
40
GERAGDGAPS
50
RAVAGASAMF
60
AGLQDLGVAN
70
GEDLKETLTN
80
CTEPLKAIEQ
90
FQTENGVLLP
100
SLQSALPFLD
110
LHGTPRLEFH
120
QSVFDELRDK
130
LLERVSAIAS
140
EGKAEERYKK
150
LEDLLEKSFS
160
LVKMPSLQPV
170
VMCVMKHLPK
180
VPEKKLKLVM
190
ADKELYRACA
200
VEVKRQIWQD
210
NQALFGDEVS
220
PLLKQYILEK
230
ESALFSTELS
240
VLHNFFSPSP
250
KTRRQGEVVQ
260
RLTRMVGKNV
270
KLYDMVLQFL
280
RTLFLRTRNV
290
HYCTLRAELL
300
MSLHDLDVGE
310
ICTVDPCHKF
320
TWCLDACIRE
330
RFVDSKRARE
340
LQGFLDGVKK
350
GQEQVLGDLS
360
MILCDPFAIN
370
TLALSTVRHL
380
QELVGQETLP
390
RDSPDLLLLL
400
RLLALGQGAW
410
DMIDSQVFKE
420
PKMEVELITR
430
FLPMLMSFLV
440
DDYTFNVDQK
450
LPAEEKAPVS
460
YPNTLPESFT
470
KFLQEQRMAC
480
EVGLYYVLHI
490
TKQRNKNALL
500
RLLPGLVETF
510
GDLAFGDIFL
520
HLLTGNLALL
530
ADEFALEDFC
540
SSLFDGFFLT
550
ASPRKENVHR
560
HALRLLIHLH
570
PRVAPSKLEA
580
LQKALEPTGQ
590
SGEAVKELYS
600
QLGEKLEQLD
610
HRKPSPAQAA
620
ETPALELPLP
SVPAPAPL
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0032021 |
NELF complex |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Molecular Function |
GO:0003723 |
RNA binding |
| Biological Process |
GO:0008283 |
cell population proliferation |
| Biological Process |
GO:2000737 |
negative regulation of stem cell differentiation |
| Biological Process |
GO:0034244 |
negative regulation of transcription elongation by RNA polymerase II |
| Biological Process |
GO:0048863 |
stem cell differentiation |
| Biological Process |
GO:0160239 |
transcription pausing by RNA polymerase II |
Reference
[1] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.