Search Results

Overview

Uniprot ID	Q92979
Protein Name	Ribosomal RNA small subunit methyltransferase NEP1
Gene Name	EMG1
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
28	DWDALPPKRPRLGAG
37	PRLGAGNKIGGRRLI

Function

S-adenosyl-L-methionine-dependent pseudouridine N(1)-methyltransferase that methylates pseudouridine at position 1248 (Psi1248) in 18S rRNA. Involved the biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi) conserved in eukaryotic 18S rRNA. Is not able to methylate uridine at this position (PubMed:20047967). Also has an essential role in 40S ribosomal subunit biogenesis independent on its methyltransferase activity, facilitating the incorporation of ribosomal protein S19 during the formation of pre-ribosomes (By similarity). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed:34516797)

Protein Sequence

10 MAAPSDGFKP 20 RERSGGEQAQ 30 DWDALPPKRP 40 RLGAGNKIGG 50 RRLIVVLEGA 60 SLETVKVGKT 70 YELLNCDKHK 80 SILLKNGRDP 90 GEARPDITHQ 100 SLLMLMDSPL 110 NRAGLLQVYI 120 HTQKNVLIEV 130 NPQTRIPRTF 140 DRFCGLMVQL 150 LHKLSVRAAD 160 GPQKLLKVIK 170 NPVSDHFPVG 180 CMKVGTSFSI 190 PVVSDVRELV 200 PSSDPIVFVV 210 GAFAHGKVSV 220 EYTEKMVSIS 230 NYPLSAALTC 240 AKLTTAFEEV WGVI

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005737	cytoplasm
Cellular Component	GO:0005730	nucleolus
Cellular Component	GO:0005654	nucleoplasm
Cellular Component	GO:0005634	nucleus
Cellular Component	GO:0032040	small-subunit processome
Molecular Function	GO:0042802	identical protein binding
Molecular Function	GO:0003723	RNA binding
Molecular Function	GO:0070037	rRNA (pseudouridine) methyltransferase activity
Molecular Function	GO:0019843	rRNA binding
Biological Process	GO:0030490	maturation of SSU-rRNA
Biological Process	GO:0042274	ribosomal small subunit biogenesis
Biological Process	GO:0070475	rRNA base methylation
Biological Process	GO:0006364	rRNA processing

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[4] Bao Q, Wan N, He Z, Cao J, Yuan W et al.. Subcellular Proteomic Mapping of Lysine Lactylation.. J Am Soc Mass Spectrom 35(12):3221-3232. 2024 Dec 4. PMID: 39569522.

[5] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.