Search Results

Overview

Uniprot IDQ93008
Protein NameUbiquitin carboxyl-terminal hydrolase 9X
Gene NameUSP9X
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
703 ACFKWYSKLMGDEPD

Function

Deubiquitinase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins (PubMed:18254724, PubMed:19135894, PubMed:22371489, PubMed:25944111, PubMed:29626158, PubMed:30914461, PubMed:37454738). May therefore play an important regulatory role at the level of protein turnover by preventing degradation of proteins through the removal of conjugated ubiquitin (PubMed:18254724, PubMed:19135894, PubMed:22371489, PubMed:25944111, PubMed:29626158, PubMed:30914461, PubMed:37454738). Specifically hydrolyzes 'Lys-11'-, followed by 'Lys-63'-, 'Lys-48'- and 'Lys-6'-linked polyubiquitins chains (PubMed:30914461). Essential component of TGF-beta/BMP signaling cascade (PubMed:19135894). Specifically deubiquitinates monoubiquitinated SMAD4, opposing the activity of E3 ubiquitin-protein ligase TRIM33 (PubMed:19135894). Deubiquitinates alkylation repair enzyme ALKBH3 (PubMed:25944111). OTUD4 recruits USP7 and USP9X to stabilize ALKBH3, thereby promoting the repair of alkylated DNA lesions (PubMed:25944111). Deubiquitinates RNA demethylase enzyme ALKBH5, promoting its stability (PubMed:37454738). Deubiquitinates mTORC2 complex component RICTOR at 'Lys-294' by removing 'Lys-63'-linked polyubiquitin chains, stabilizing RICTOR and enhancing its binding to MTOR, thus promoting mTORC2 complex assembly (PubMed:33378666). Regulates chromosome alignment and segregation in mitosis by regulating the localization of BIRC5/survivin to mitotic centromeres (PubMed:16322459). Involved in axonal growth and neuronal cell migration (PubMed:24607389). Regulates cellular clock function by enhancing the protein stability and transcriptional activity of the core circadian protein BMAL1 via its deubiquitinating activity (PubMed:29626158). Acts as a regulator of peroxisome import by mediating deubiquitination of PEX5: specifically deubiquitinates PEX5 monoubiquitinated at 'Cys-11' following its retrotranslocation into the cytosol, resetting PEX5 for a subsequent import cycle (PubMed:22371489). Deubiquitinates PEG10 (By similarity). Inhibits the activation of the Hippo signaling pathway via deubiquitination of AMOTL2 at 'Lys-347' and 'Lys-408' which prohibits its interaction with and activation of LATS2. Loss of LATS2 activation and subsequent loss of YAP1 phosphorylation results in an increase in YAP1-driven transcription of target genes (PubMed:26598551, PubMed:34404733)

Protein Sequence

10 MTATTRGSPV 20 GGNDNQGQAP 30 DGQSQPPLQQ 40 NQTSSPDSSN 50 ENSPATPPDE 60 QGQGDAPPQL 70 EDEEPAFPHT 80 DLAKLDDMIN 90 RPRWVVPVLP 100 KGELEVLLEA 110 AIDLSKKGLD 120 VKSEACQRFF 130 RDGLTISFTK 140 ILTDEAVSGW 150 KFEIHRCIIN 160 NTHRLVELCV 170 AKLSQDWFPL 180 LELLAMALNP 190 HCKFHIYNGT 200 RPCESVSSSV 210 QLPEDELFAR 220 SPDPRSPKGW 230 LVDLLNKFGT 240 LNGFQILHDR 250 FINGSALNVQ 260 IIAALIKPFG 270 QCYEFLTLHT 280 VKKYFLPIIE 290 MVPQFLENLT 300 DEELKKEAKN 310 EAKNDALSMI 320 IKSLKNLASR 330 VPGQEETVKN 340 LEIFRLKMIL 350 RLLQISSFNG 360 KMNALNEVNK 370 VISSVSYYTH 380 RHGNPEEEEW 390 LTAERMAEWI 400 QQNNILSIVL 410 RDSLHQPQYV 420 EKLEKILRFV 430 IKEKALTLQD 440 LDNIWAAQAG 450 KHEAIVKNVH 460 DLLAKLAWDF 470 SPEQLDHLFD 480 CFKASWTNAS 490 KKQREKLLEL 500 IRRLAEDDKD 510 GVMAHKVLNL 520 LWNLAHSDDV 530 PVDIMDLALS 540 AHIKILDYSC 550 SQDRDTQKIQ 560 WIDRFIEELR 570 TNDKWVIPAL 580 KQIREICSLF 590 GEAPQNLSQT 600 QRSPHVFYRH 610 DLINQLQHNH 620 ALVTLVAENL 630 ATYMESMRLY 640 ARDHEDYDPQ 650 TVRLGSRYSH 660 VQEVQERLNF 670 LRFLLKDGQL 680 WLCAPQAKQI 690 WKCLAENAVY 700 LCDREACFKW 710 YSKLMGDEPD 720 LDPDINKDFF 730 ESNVLQLDPS 740 LLTENGMKCF 750 ERFFKAVNCR 760 EGKLVAKRRA 770 YMMDDLELIG 780 LDYLWRVVIQ 790 SNDDIASRAI 800 DLLKEIYTNL 810 GPRLQVNQVV 820 IHEDFIQSCF 830 DRLKASYDTL 840 CVLDGDKDSV 850 NCARQEAVRM 860 VRVLTVLREY 870 INECDSDYHE 880 ERTILPMSRA 890 FRGKHLSFVV 900 RFPNQGRQVD 910 DLEVWSHTND 920 TIGSVRRCIL 930 NRIKANVAHT 940 KIELFVGGEL 950 IDPADDRKLI 960 GQLNLKDKSL 970 ITAKLTQISS 980 NMPSSPDSSS 990 DSSTGSPGNH 1000 GNHYSDGPNP 1010 EVESCLPGVI 1020 MSLHPRYISF 1030 LWQVADLGSS 1040 LNMPPLRDGA 1050 RVLMKLMPPD 1060 STTIEKLRAI 1070 CLDHAKLGES 1080 SLSPSLDSLF 1090 FGPSASQVLY 1100 LTEVVYALLM 1110 PAGAPLADDS 1120 SDFQFHFLKS 1130 GGLPLVLSML 1140 TRNNFLPNAD 1150 METRRGAYLN 1160 ALKIAKLLLT 1170 AIGYGHVRAV 1180 AEACQPGVEG 1190 VNPMTQINQV 1200 THDQAVVLQS 1210 ALQSIPNPSS 1220 ECMLRNVSVR 1230 LAQQISDEAS 1240 RYMPDICVIR 1250 AIQKIIWASG 1260 CGSLQLVFSP 1270 NEEITKIYEK 1280 TNAGNEPDLE 1290 DEQVCCEALE 1300 VMTLCFALIP 1310 TALDALSKEK 1320 AWQTFIIDLL 1330 LHCHSKTVRQ 1340 VAQEQFFLMC 1350 TRCCMGHRPL 1360 LFFITLLFTV 1370 LGSTARERAK 1380 HSGDYFTLLR 1390 HLLNYAYNSN 1400 INVPNAEVLL 1410 NNEIDWLKRI 1420 RDDVKRTGET 1430 GIEETILEGH 1440 LGVTKELLAF 1450 QTSEKKFHIG 1460 CEKGGANLIK 1470 ELIDDFIFPA 1480 SNVYLQYMRN 1490 GELPAEQAIP 1500 VCGSPPTINA 1510 GFELLVALAV 1520 GCVRNLKQIV 1530 DSLTEMYYIG 1540 TAITTCEALT 1550 EWEYLPPVGP 1560 RPPKGFVGLK 1570 NAGATCYMNS 1580 VIQQLYMIPS 1590 IRNGILAIEG 1600 TGSDVDDDMS 1610 GDEKQDNESN 1620 VDPRDDVFGY 1630 PQQFEDKPAL 1640 SKTEDRKEYN 1650 IGVLRHLQVI 1660 FGHLAASRLQ 1670 YYVPRGFWKQ 1680 FRLWGEPVNL 1690 REQHDALEFF 1700 NSLVDSLDEA 1710 LKALGHPAML 1720 SKVLGGSFAD 1730 QKICQGCPHR 1740 YECEESFTTL 1750 NVDIRNHQNL 1760 LDSLEQYVKG 1770 DLLEGANAYH 1780 CEKCNKKVDT 1790 VKRLLIKKLP 1800 PVLAIQLKRF 1810 DYDWERECAI 1820 KFNDYFEFPR 1830 ELDMEPYTVA 1840 GVAKLEGDNV 1850 NPESQLIQQS 1860 EQSESETAGS 1870 TKYRLVGVLV 1880 HSGQASGGHY 1890 YSYIIQRNGG 1900 DGERNRWYKF 1910 DDGDVTECKM 1920 DDDEEMKNQC 1930 FGGEYMGEVF 1940 DHMMKRMSYR 1950 RQKRWWNAYI 1960 LFYERMDTID 1970 QDDELIRYIS 1980 ELAITTRPHQ 1990 IIMPSAIERS 2000 VRKQNVQFMH 2010 NRMQYSMEYF 2020 QFMKKLLTCN 2030 GVYLNPPPGQ 2040 DHLLPEAEEI 2050 TMISIQLAAR 2060 FLFTTGFHTK 2070 KVVRGSASDW 2080 YDALCILLRH 2090 SKNVRFWFAH 2100 NVLFNVSNRF 2110 SEYLLECPSA 2120 EVRGAFAKLI 2130 VFIAHFSLQD 2140 GPCPSPFASP 2150 GPSSQAYDNL 2160 SLSDHLLRAV 2170 LNLLRREVSE 2180 HGRHLQQYFN 2190 LFVMYANLGV 2200 AEKTQLLKLS 2210 VPATFMLVSL 2220 DEGPGPPIKY 2230 QYAELGKLYS 2240 VVSQLIRCCN 2250 VSSRMQSSIN 2260 GNPPLPNPFG 2270 DPNLSQPIMP 2280 IQQNVADILF 2290 VRTSYVKKII 2300 EDCSNSEETV 2310 KLLRFCCWEN 2320 PQFSSTVLSE 2330 LLWQVAYSYT 2340 YELRPYLDLL 2350 LQILLIEDSW 2360 QTHRIHNALK 2370 GIPDDRDGLF 2380 DTIQRSKNHY 2390 QKRAYQCIKC 2400 MVALFSNCPV 2410 AYQILQGNGD 2420 LKRKWTWAVE 2430 WLGDELERRP 2440 YTGNPQYTYN 2450 NWSPPVQSNE 2460 TSNGYFLERS 2470 HSARMTLAKA 2480 CELCPEEEPD 2490 DQDAPDEHES 2500 PPPEDAPLYP 2510 HSPGSQYQQN 2520 NHVHGQPYTG 2530 PAAHHMNNPQ 2540 RTGQRAQENY 2550 EGSEEVSPPQ TKDQ

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005930 axoneme
Cellular Component GO:0005813 centrosome
Cellular Component GO:0005929 cilium
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0030426 growth cone
Cellular Component GO:0016020 membrane
Cellular Component GO:0005634 nucleus
Molecular Function GO:0070410 co-SMAD binding
Molecular Function GO:0004843 cysteine-type deubiquitinase activity
Molecular Function GO:0004197 cysteine-type endopeptidase activity
Molecular Function GO:0008234 cysteine-type peptidase activity
Molecular Function GO:0101005 deubiquitinase activity
Molecular Function GO:0180017 K11-linked deubiquitinase activity
Molecular Function GO:1990380 K48-linked deubiquitinase activity
Molecular Function GO:0061578 K63-linked deubiquitinase activity
Molecular Function GO:0140313 molecular sequestering activity
Biological Process GO:1990000 amyloid fibril formation
Biological Process GO:0048675 axon extension
Biological Process GO:0030509 BMP signaling pathway
Biological Process GO:0051301 cell division
Biological Process GO:0016477 cell migration
Biological Process GO:0007059 chromosome segregation
Biological Process GO:0060271 cilium assembly
Biological Process GO:0061824 cytosolic ciliogenesis
Biological Process GO:0006307 DNA alkylation repair
Biological Process GO:0007292 female gamete generation
Biological Process GO:0008104 intracellular protein localization
Biological Process GO:0035520 monoubiquitinated protein deubiquitination
Biological Process GO:0032435 negative regulation of proteasomal ubiquitin-dependent protein catabolic process
Biological Process GO:0000122 negative regulation of transcription by RNA polymerase II
Biological Process GO:0001764 neuron migration
Biological Process GO:0032092 positive regulation of protein binding
Biological Process GO:1904515 positive regulation of TORC2 signaling
Biological Process GO:0016579 protein deubiquitination
Biological Process GO:0071947 protein deubiquitination involved in ubiquitin-dependent protein catabolic process
Biological Process GO:0016562 protein import into peroxisome matrix, receptor recycling
Biological Process GO:0070536 protein K63-linked deubiquitination
Biological Process GO:0050821 protein stabilization
Biological Process GO:0016567 protein ubiquitination
Biological Process GO:0042752 regulation of circadian rhythm
Biological Process GO:0031647 regulation of protein stability
Biological Process GO:0048511 rhythmic process
Biological Process GO:0007179 transforming growth factor beta receptor signaling pathway

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.