Search Results

Overview

Uniprot ID	Q93074
Protein Name	Mediator of RNA polymerase II transcription subunit 12
Gene Name	MED12
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
1643	RQLLPLPKQTRDVIT
1664	LIDTKGNKIAGFDSI

Function

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway

Protein Sequence

10 MAAFGILSYE 20 HRPLKRPRLG 30 PPDVYPQDPK 40 QKEDELTALN 50 VKQGFNNQPA 60 VSGDEHGSAK 70 NVSFNPAKIS 80 SNFSSIIAEK 90 LRCNTLPDTG 100 RRKPQVNQKD 110 NFWLVTARSQ 120 SAINTWFTDL 130 AGTKPLTQLA 140 KKVPIFSKKE 150 EVFGYLAKYT 160 VPVMRAAWLI 170 KMTCAYYAAI 180 SETKVKKRHV 190 DPFMEWTQII 200 TKYLWEQLQK 210 MAEYYRPGPA 220 GSGGCGSTIG 230 PLPHDVEVAI 240 RQWDYTEKLA 250 MFMFQDGMLD 260 RHEFLTWVLE 270 CFEKIRPGED 280 ELLKLLLPLL 290 LRYSGEFVQS 300 AYLSRRLAYF 310 CTRRLALQLD 320 GVSSHSSHVI 330 SAQSTSTLPT 340 TPAPQPPTSS 350 TPSTPFSDLL 360 MCPQHRPLVF 370 GLSCILQTIL 380 LCCPSALVWH 390 YSLTDSRIKT 400 GSPLDHLPIA 410 PSNLPMPEGN 420 SAFTQQVRAK 430 LREIEQQIKE 440 RGQAVEVRWS 450 FDKCQEATAG 460 FTIGRVLHTL 470 EVLDSHSFER 480 SDFSNSLDSL 490 CNRIFGLGPS 500 KDGHEISSDD 510 DAVVSLLCEW 520 AVSCKRSGRH 530 RAMVVAKLLE 540 KRQAEIEAER 550 CGESEAADEK 560 GSIASGSLSA 570 PSAPIFQDVL 580 LQFLDTQAPM 590 LTDPRSESER 600 VEFFNLVLLF 610 CELIRHDVFS 620 HNMYTCTLIS 630 RGDLAFGAPG 640 PRPPSPFDDP 650 ADDPEHKEAE 660 GSSSSKLEDP 670 GLSESMDIDP 680 SSSVLFEDME 690 KPDFSLFSPT 700 MPCEGKGSPS 710 PEKPDVEKEV 720 KPPPKEKIEG 730 TLGVLYDQPR 740 HVQYATHFPI 750 PQEESCSHEC 760 NQRLVVLFGV 770 GKQRDDARHA 780 IKKITKDILK 790 VLNRKGTAET 800 DQLAPIVPLN 810 PGDLTFLGGE 820 DGQKRRRNRP 830 EAFPTAEDIF 840 AKFQHLSHYD 850 QHQVTAQVSR 860 NVLEQITSFA 870 LGMSYHLPLV 880 QHVQFIFDLM 890 EYSLSISGLI 900 DFAIQLLNEL 910 SVVEAELLLK 920 SSDLVGSYTT 930 SLCLCIVAVL 940 RHYHACLILN 950 QDQMAQVFEG 960 LCGVVKHGMN 970 RSDGSSAERC 980 ILAYLYDLYT 990 SCSHLKNKFG 1000 ELFSDFCSKV 1010 KNTIYCNVEP 1020 SESNMRWAPE 1030 FMIDTLENPA 1040 AHTFTYTGLG 1050 KSLSENPANR 1060 YSFVCNALMH 1070 VCVGHHDPDR 1080 VNDIAILCAE 1090 LTGYCKSLSA 1100 EWLGVLKALC 1110 CSSNNGTCGF 1120 NDLLCNVDVS 1130 DLSFHDSLAT 1140 FVAILIARQC 1150 LLLEDLIRCA 1160 AIPSLLNAAC 1170 SEQDSEPGAR 1180 LTCRILLHLF 1190 KTPQLNPCQS 1200 DGNKPTVGIR 1210 SSCDRHLLAA 1220 SQNRIVDGAV 1230 FAVLKAVFVL 1240 GDAELKGSGF 1250 TVTGGTEELP 1260 EEEGGGGSGG 1270 RRQGGRNISV 1280 ETASLDVYAK 1290 YVLRSICQQE 1300 WVGERCLKSL 1310 CEDSNDLQDP 1320 VLSSAQAQRL 1330 MQLICYPHRL 1340 LDNEDGENPQ 1350 RQRIKRILQN 1360 LDQWTMRQSS 1370 LELQLMIKQT 1380 PNNEMNSLLE 1390 NIAKATIEVF 1400 QQSAETGSSS 1410 GSTASNMPSS 1420 SKTKPVLSSL 1430 ERSGVWLVAP 1440 LIAKLPTSVQ 1450 GHVLKAAGEE 1460 LEKGQHLGSS 1470 SRKERDRQKQ 1480 KSMSLLSQQP 1490 FLSLVLTCLK 1500 GQDEQREGLL 1510 TSLYSQVHQI 1520 VNNWRDDQYL 1530 DDCKPKQLMH 1540 EALKLRLNLV 1550 GGMFDTVQRS 1560 TQQTTEWAML 1570 LLEIIISGTV 1580 DMQSNNELFT 1590 TVLDMLSVLI 1600 NGTLAADMSS 1610 ISQGSMEENK 1620 RAYMNLAKKL 1630 QKELGERQSD 1640 SLEKVRQLLP 1650 LPKQTRDVIT 1660 CEPQGSLIDT 1670 KGNKIAGFDS 1680 IFKKEGLQVS 1690 TKQKISPWDL 1700 FEGLKPSAPL 1710 SWGWFGTVRV 1720 DRRVARGEEQ 1730 QRLLLYHTHL 1740 RPRPRAYYLE 1750 PLPLPPEDEE 1760 PPAPTLLEPE 1770 KKAPEPPKTD 1780 KPGAAPPSTE 1790 ERKKKSTKGK 1800 KRSQPATKTE 1810 DYGMGPGRSG 1820 PYGVTVPPDL 1830 LHHPNPGSIT 1840 HLNYRQGSIG 1850 LYTQNQPLPA 1860 GGPRVDPYRP 1870 VRLPMQKLPT 1880 RPTYPGVLPT 1890 TMTGVMGLEP 1900 SSYKTSVYRQ 1910 QQPAVPQGQR 1920 LRQQLQQSQG 1930 MLGQSSVHQM 1940 TPSSSYGLQT 1950 SQGYTPYVSH 1960 VGLQQHTGPA 1970 GTMVPPSYSS 1980 QPYQSTHPST 1990 NPTLVDPTRH 2000 LQQRPSGYVH 2010 QQAPTYGHGL 2020 TSTQRFSHQT 2030 LQQTPMISTM 2040 TPMSAQGVQA 2050 GVRSTAILPE 2060 QQQQQQQQQQ 2070 QQQQQQQQQQ 2080 QQQQQQYHIR 2090 QQQQQQILRQ 2100 QQQQQQQQQQ 2110 QQQQQQQQQQ 2120 QQQQQHQQQQ 2130 QQQAAPPQPQ 2140 PQSQPQFQRQ 2150 GLQQTQQQQQ 2160 TAALVRQLQQ 2170 QLSNTQPQPS TNIFGRY

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0016592	mediator complex
Cellular Component	GO:0016020	membrane
Cellular Component	GO:0005654	nucleoplasm
Cellular Component	GO:0005634	nucleus
Cellular Component	GO:0000151	ubiquitin ligase complex
Molecular Function	GO:0008013	beta-catenin binding
Molecular Function	GO:0003682	chromatin binding
Molecular Function	GO:0046966	nuclear thyroid hormone receptor binding
Molecular Function	GO:0042809	nuclear vitamin D receptor binding
Molecular Function	GO:0000978	RNA polymerase II cis-regulatory region sequence-specific DNA binding
Molecular Function	GO:0003713	transcription coactivator activity
Molecular Function	GO:0003712	transcription coregulator activity
Molecular Function	GO:0061630	ubiquitin protein ligase activity
Biological Process	GO:0006351	DNA-templated transcription
Biological Process	GO:0045893	positive regulation of DNA-templated transcription
Biological Process	GO:0045944	positive regulation of transcription by RNA polymerase II
Biological Process	GO:0060261	positive regulation of transcription initiation by RNA polymerase II
Biological Process	GO:0016567	protein ubiquitination

Reference

[1] Lin Y, Chen M, Wang D, Yu Y, Chen R et al.. Multi-Proteomic Analysis Reveals the Effect of Protein Lactylation on Matrix and Cholesterol Metabolism in Tendinopathy.. J Proteome Res 22(6):1712-1722. 2023 Jun 2. PMID: 37159428.

[2] Cheng Z, Huang H, Li M, Chen Y. Proteomic analysis identifies PFKP lactylation in SW480 colon cancer cells.. iScience 27(1):108645. 2024 Jan 19. PMID: 38155775.

[3] Bao Q, Wan N, He Z, Cao J, Yuan W et al.. Subcellular Proteomic Mapping of Lysine Lactylation.. J Am Soc Mass Spectrom 35(12):3221-3232. 2024 Dec 4. PMID: 39569522.

[4] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.