Overview
| Uniprot ID | Q969P6 |
|---|
| Protein Name | DNA topoisomerase I, mitochondrial |
|---|
| Gene Name | TOP1MT |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 394 |
LQLFMENKDPRDDLF |
Function
Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity)
Protein Sequence
10
MRVVRLLRLR
20
AALTLLGEVP
30
RRPASRGVPG
40
SRRTQKGSGA
50
RWEKEKHEDG
60
VKWRQLEHKG
70
PYFAPPYEPL
80
PDGVRFFYEG
90
RPVRLSVAAE
100
EVATFYGRML
110
DHEYTTKEVF
120
RKNFFNDWRK
130
EMAVEEREVI
140
KSLDKCDFTE
150
IHRYFVDKAA
160
ARKVLSREEK
170
QKLKEEAEKL
180
QQEFGYCILD
190
GHQEKIGNFK
200
IEPPGLFRGR
210
GDHPKMGMLK
220
RRITPEDVVI
230
NCSRDSKIPE
240
PPAGHQWKEV
250
RSDNTVTWLA
260
AWTESVQNSI
270
KYIMLNPCSK
280
LKGETAWQKF
290
ETARRLRGFV
300
DEIRSQYRAD
310
WKSREMKTRQ
320
RAVALYFIDK
330
LALRAGNEKE
340
DGEAADTVGC
350
CSLRVEHVQL
360
HPEADGCQHV
370
VEFDFLGKDC
380
IRYYNRVPVE
390
KPVYKNLQLF
400
MENKDPRDDL
410
FDRLTTTSLN
420
KHLQELMDGL
430
TAKVFRTYNA
440
SITLQEQLRA
450
LTRAEDSIAA
460
KILSYNRANR
470
VVAILCNHQR
480
ATPSTFEKSM
490
QNLQTKIQAK
500
KEQVAEARAE
510
LRRARAEHKA
520
QGDGKSRSVL
530
EKKRRLLEKL
540
QEQLAQLSVQ
550
ATDKEENKQV
560
ALGTSKLNYL
570
DPRISIAWCK
580
RFRVPVEKIY
590
SKTQRERFAW
600
ALAMAGEDFE
F
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005694 |
chromosome |
| Cellular Component |
GO:0042645 |
mitochondrial nucleoid |
| Cellular Component |
GO:0005739 |
mitochondrion |
| Cellular Component |
GO:0005634 |
nucleus |
| Molecular Function |
GO:0003677 |
DNA binding |
| Molecular Function |
GO:0003917 |
DNA topoisomerase type I (single strand cut, ATP-independent) activity |
| Biological Process |
GO:0006260 |
DNA replication |
| Biological Process |
GO:0006265 |
DNA topological change |
Reference
[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.