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Overview

Uniprot IDQ96AX1
Protein NameVacuolar protein sorting-associated protein 33A
Gene NameVPS33A
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
210 ANMMIRMKREFTGSQ
429 QKVLDYYKREILQTY

Function

Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations (PubMed:23351085, PubMed:24554770, PubMed:25266290, PubMed:25783203). Required for fusion of endosomes and autophagosomes with lysosomes; the function is dependent on its association with VPS16 but not VIPAS39 (PubMed:25783203). The function in autophagosome-lysosome fusion implicates STX17 but not UVRAG (PubMed:24554770)

Protein Sequence

10 MAAHLSYGRV 20 NLNVLREAVR 30 RELREFLDKC 40 AGSKAIVWDE 50 YLTGPFGLIA 60 QYSLLKEHEV 70 EKMFTLKGNR 80 LPAADVKNII 90 FFVRPRLELM 100 DIIAENVLSE 110 DRRGPTRDFH 120 ILFVPRRSLL 130 CEQRLKDLGV 140 LGSFIHREEY 150 SLDLIPFDGD 160 LLSMESEGAF 170 KECYLEGDQT 180 SLYHAAKGLM 190 TLQALYGTIP 200 QIFGKGECAR 210 QVANMMIRMK 220 REFTGSQNSI 230 FPVFDNLLLL 240 DRNVDLLTPL 250 ATQLTYEGLI 260 DEIYGIQNSY 270 VKLPPEKFAP 280 KKQGDGGKDL 290 PTEAKKLQLN 300 SAEELYAEIR 310 DKNFNAVGSV 320 LSKKAKIISA 330 AFEERHNAKT 340 VGEIKQFVSQ 350 LPHMQAARGS 360 LANHTSIAEL 370 IKDVTTSEDF 380 FDKLTVEQEF 390 MSGIDTDKVN 400 NYIEDCIAQK 410 HSLIKVLRLV 420 CLQSVCNSGL 430 KQKVLDYYKR 440 EILQTYGYEH 450 ILTLHNLEKA 460 GLLKPQTGGR 470 NNYPTIRKTL 480 RLWMDDVNEQ 490 NPTDISYVYS 500 GYAPLSVRLA 510 QLLSRPGWRS 520 IEEVLRILPG 530 PHFEERQPLP 540 TGLQKKRQPG 550 ENRVTLIFFL 560 GGVTFAEIAA 570 LRFLSQLEDG 580 GTEYVIATTK 590 LMNGTSWIEA LMEKPF

Gene Ontology

Classification GO ID Description
Cellular Component GO:0030897 HOPS complex
Cellular Component GO:0005776 autophagosome
Cellular Component GO:0030136 clathrin-coated vesicle
Cellular Component GO:0033263 CORVET complex
Cellular Component GO:0005769 early endosome
Cellular Component GO:0010008 endosome membrane
Cellular Component GO:0005770 late endosome
Cellular Component GO:0031902 late endosome membrane
Cellular Component GO:0005765 lysosomal membrane
Cellular Component GO:0005764 lysosome
Cellular Component GO:0048471 perinuclear region of cytoplasm
Biological Process GO:0097352 autophagosome maturation
Biological Process GO:0061909 autophagosome-lysosome fusion
Biological Process GO:0034058 endosomal vesicle fusion
Biological Process GO:0008333 endosome to lysosome transport
Biological Process GO:0006886 intracellular protein transport
Biological Process GO:0032418 lysosome localization
Biological Process GO:0032400 melanosome localization
Biological Process GO:0030220 platelet formation
Biological Process GO:0048070 regulation of developmental pigmentation
Biological Process GO:0035751 regulation of lysosomal lumen pH
Biological Process GO:0035542 regulation of SNARE complex assembly
Biological Process GO:0016192 vesicle-mediated transport

Reference

[1] Cheng Z, Huang H, Li M, Chen Y. Proteomic analysis identifies PFKP lactylation in SW480 colon cancer cells.. iScience 27(1):108645. 2024 Jan 19. PMID: 38155775.

[2] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.