Search Results
Overview
| Uniprot ID | Q96B01 |
|---|---|
| Protein Name | RAD51-associated protein 1 |
| Gene Name | RAD51AP1 |
| Organism | Homo sapiens |
Kla Sites from experimental identification
| Position | Flanking peptide |
|---|---|
| 169 | DVGGVQGKRKAASKA |
| 175 | GKRKAASKAAAQQRK |
| 253 | KEKKSKSKCNALVTS |
| 277 | SESQSLPKKVSLSSD |
| 278 | ESQSLPKKVSLSSDT |
| 300 | RSPSAESKKPKWVPP |
Function
Structure-specific DNA-binding protein involved in DNA repair by promoting RAD51-mediated homologous recombination (PubMed:17996710, PubMed:17996711, PubMed:20871616, PubMed:25288561, PubMed:26323318). Acts by stimulating D-Loop formation by RAD51: specifically enhances joint molecule formation through its structure-specific DNA interaction and its interaction with RAD51 (PubMed:17996710, PubMed:17996711). Binds single-stranded DNA (ssDNA), double-stranded DNA (dsDNA) and secondary DNA structures, such as D-loop structures: has a strong preference for branched-DNA structures that are obligatory intermediates during joint molecule formation (PubMed:17996710, PubMed:17996711, PubMed:22375013, PubMed:9396801). Cooperates with WDR48/UAF1 to stimulate RAD51-mediated homologous recombination: both WDR48/UAF1 and RAD51AP1 have coordinated role in DNA-binding during homologous recombination and DNA repair (PubMed:27239033, PubMed:27463890, PubMed:32350107). WDR48/UAF1 and RAD51AP1 also have a coordinated role in DNA-binding to promote USP1-mediated deubiquitination of FANCD2 (PubMed:31253762). Also involved in meiosis by promoting DMC1-mediated homologous meiotic recombination (PubMed:21307306). Key mediator of alternative lengthening of telomeres (ALT) pathway, a homology-directed repair mechanism of telomere elongation that controls proliferation in aggressive cancers, by stimulating homologous recombination (PubMed:31400850). May also bind RNA; additional evidences are however required to confirm RNA-binding in vivo (PubMed:9396801)
Protein Sequence
Gene Ontology
| Classification | GO ID | Description |
|---|---|---|
| Cellular Component | GO:0000785 | chromatin |
| Cellular Component | GO:0005694 | chromosome |
| Cellular Component | GO:0000781 | chromosome, telomeric region |
| Cellular Component | GO:0005654 | nucleoplasm |
| Cellular Component | GO:0005634 | nucleus |
| Cellular Component | GO:0032991 | protein-containing complex |
| Molecular Function | GO:0062037 | D-loop DNA binding |
| Molecular Function | GO:0003677 | DNA binding |
| Molecular Function | GO:0000217 | DNA secondary structure binding |
| Molecular Function | GO:0003690 | double-stranded DNA binding |
| Molecular Function | GO:0003723 | RNA binding |
| Molecular Function | GO:0003697 | single-stranded DNA binding |
| Biological Process | GO:0071479 | cellular response to ionizing radiation |
| Biological Process | GO:0006974 | DNA damage response |
| Biological Process | GO:0006281 | DNA repair |
| Biological Process | GO:0000724 | double-strand break repair via homologous recombination |
| Biological Process | GO:0036297 | interstrand cross-link repair |
| Biological Process | GO:0051321 | meiotic cell cycle |
| Biological Process | GO:1905168 | positive regulation of double-strand break repair via homologous recombination |
| Biological Process | GO:0010845 | positive regulation of reciprocal meiotic recombination |
| Biological Process | GO:0010569 | regulation of double-strand break repair via homologous recombination |
Reference
[1] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.
[2] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.